Haplophyllum suaveolens Varyetelerinin Antioksidan Aktivitesi ve Sekonder Metabolitleri Üzerine Farklı Çözücülerin Etkisi

Bu çalışmada, Haplophyllum suaveolens varyetelerine ait methanol ve etanol ekstraktlarının toplam biyoaktif içerikleri ile antioksidan aktivite seviyelerinin belirlenmesi amaçlanmıştır. Ekstraksiyon işleminde Haplophyllum suaveolens varyeteleri olan Haplophyllum suaveolens (DC.) G.Don var. suaveolens (HS) ve endemik Haplophyllum suaveolens var. cilicicum (Boiss.) C.C.Townsend (HC) taksonlarının herba kısımları kullanılmıştır. Toplam fenolik içerik Folin-Ciocalteu metotuna göre belirlenmiş ve her bir ekstrakttaki miktarları mg gallik asit eş değer (GAE) g-1 ekstrakt olarak ifade edilmiştir. Ekstraktların toplam fenolik içerikleri 39.54-44.16 mg GAE g-1 ekstrakt arasında değişim göstermiştir. Toplam flavonoid içerikleri ise mg kuersetin eş değer (QE) g-1 ekstrakt olarak ölçülmüş ve 17.30-41.40 mg QE g-1 ekstrakt arasında değişim göstermiştir. Çalışmada, HS ve HC taksonlarının methanol ekstraktları en yüksek 2,2-difenil-1-pikrilhidrazil (DPPH, sırayla % 79.76 ve % 75.69) ve 2,2’-azinobis (3-etil-bezotiazolin 6 sulfonat) (ABTS, sırayla % 54.74 ve % 55.13) radikal kovucu aktiviteleri göstermiştir. Böylece, Haplophyllum suaveolens varyetelerinin güçlü radikal kovucu aktivite sergilediği ve her iki varyete içinde metanol ekstraktların etanol ekstraktlarına göre daha etkili olduğu tespit edilmiştir. Bu çalşma ile Haplophyllum suaveolens varyetelerinin güçlü radikal kovucu aktiviteye sahip olduğu, tıbbi ve ticari kullanım için iyi birer doğal antioksidan kaynağı olabileceği tespit edilmiştir.

Anahtar Kelimeler:

Haplophyllum L., Antioksidan aktivitesi, flavonoid, Haplophyllum L., fenolik

Effect of Different Solvents on Antioxidant Activities and Secondery Metabolites of Haplophyllum suaveolens Varieties

In this study, total bioactive contents and antioxidant activity levels of methanol and ethanol extracts of Haplophyllum suaveolens varieties were determined. Aerial parts of Haplophyllum suaveolens varieties which are Haplophyllum suaveolens (DC.) G. Don var. suaveolens (HS) and endemic Haplophyllum suaveolens var. cilicicum (Boiss.) C.C. Townsend (HC), were used in the extraction process. Total phenolic content was determined according to the Folin-Ciocalteu method and the amounts in each extract were expressed as mg gallic acid equivalent (GAE) g-1 extract. The total phenolic contents of the extracts varied between 39.54-44.16 mg GAE g-1 extract. Total flavonoid contents were measured as mg quercetin equivalent (QE) g-1 extract and varied between 17.30-41.40 mg QE g-1 extract. The methanol extracts of HS and HC exhibited maximum radical scavenging activities for DPPH radical (79.76% and 75.69%, respectively) and ABTS (54.74% and 55.13%, respectively). Thus, Haplophyllum suaveolens varieties exhibited strong radical scavenging activity and methanol extracts were found to be more effective than ethanol extracts in both varieties. In this study, it has been determined that Haplophyllum suaveolens varieties have strong radical scavenging activity and maybe good natural antioxidant sources for medical and commercial use.

Keywords:

Haplophyllum L., Antioxidant activity, flavonoid, Haplophyllum L., phenolic,

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