Ayse Gülden ADAY KAYA, Tuğba DOĞMUŞ LEHTİJARVİ, Asko Tapio LEHTİJARVİ

Türkiye’nin kuzeybatısındaki bir plantasyonda yerli ve egzotik çam türlerinin ibrelerinde görülen fungal endofitler

İzmit Kerpe Araştırma Ormanı'nda 2016 yılında Pinus sylvestris, Pinus pinaster, Pinus nigra, Pinus taeda ve Pinus radiata ib-relerinde bulunan endofitik funguslar araştırılmıştır. Her çam türünden on ağacın tepe tacının alt kısmından sistematik olarak önceki yılın ibreleri örneklenmiştir. Her bir ibre yüzeysel olarak steril edilmiş ve 0,5 cm büyüklüğünde parçalara ayrılmış daha sonra Malt Ekstrakt Agar besi ortamına yerleştirilmiştir. Gelişen izolatlardan seçilen temsili 7 izolattan DNA ekstraksiyonu ya-pılarak, 5.8S rDNA, 18S ve 28S rDNA genleri arasında yer alan iki ITS bölgesini hedef alan ITS1 ve ITS4 primerleri kullanıla-rak amplifiye edilmiştir. Amplikonlar, evrensel fungus primerleri kullanılarak her iki yönde dizilenmiştir. Toplam 1000 ibreden (her çam türünden 200 ibre) yapılan izolasyonda 750 fungus izolatı elde edilmiştir. En fazla oranda Pestalotiopsis funerea izole edilmiş, bunu Acremonium sp., Cladiosporum sp. ve Cyclaneusma minus takip etmiştir.

Anahtar Kelimeler:

Fungal topluluk, Marmara Bölgesi, ITS bölgesi

Fungal endophytes in the needles of native and exotic pine species in a planta-tion in Northwestern Türkiye

The endophytic fungi present in needles of Pinus sylvestris, Pinus pinaster, Pinus nigra, Pinus taeda and Pinus radiata were investigated in Kerpe Research Forest, İzmit in 2016. Ten trees of each pine species were sampled systematically. Previous years green needles were sampled from the lower part of the canopy, from two equally spaced positions around the tree. Each needle was surface sterilized and cut into 0.5 cm sections then individually placed onto malt extract agar plates. DNA was ex-tracted from reprensentative seven isolates and amplified using primers ITS1 and ITS4 targeting the nuclear 5.8S rDNA gene and the two ITS regions flanked between 18S and 28S rDNA genes. Amplicons sequenced in both directions using the univer-sal fungal primers ITS1 and ITS4. Isolations from a total of 1000 needles (200 from each pine species) yielded 750 fungal isolates. Pestalotiopsis funerea (Desm.) Steyaert was isolated at the highest frequency followed by Acremonium sp., Cladio-sporum sp. and Cyclaneusma minus.

Keywords:

Fungal community, Marmara Region, ITS region,

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