İbrahim Halil ELEKCİOĞLU, Halil TOKTAY, Mustafa İMREN, Adem ÖZARSLANDAN, Ece B. KASAPOĞLU

Türkiye buğday faunası için yeni bir tür, Meloidogyne artiellia (Franklin)’nın morfolojik ve moleküler yöntemlerle tanımlanması

Kök-ur nematodları (Meloidogyne spp.) dünyada ekonomik önem sahip bitki paraziti nematodları arasındadır. Meloidogyne cinsine ait nematod türleri sebzelerde ve tahıllarda önemli oranda ürün kayıplarına neden oldukları bilinmektedir. Kök ur nematodu, M. artiellia özellikle tahıllar, baklagiller ve cruciferae familyasına ait ürünlerde zarara neden olabilmektedir. Bu çalışmadaki M. artiellia izolatları Türkiye’de ilk defa Hatay ili buğday ekim alanlarından 2012 yılı üretim sezonunda toplanan toprak ve kök örneklerinden elde edilmiş olup morfolojik ve moleküler olarak tanımlamaları yapılmıştır. M. artiellia, ikinci dönem larva ve dişi bireylere ait morfolojik özellikler ve allometrik kriterler esas alınarak tanımlanmıştır. Ayrıca, mitokondriyal DNA’nın sitokrom C oksidaz alt ünitesine (COI) ait nükleotid dizilimleri kullanılarak moleküler olarak da tanımlanmış ve diğer kök ur nematodu türlerinden bariz olarak ayrıldığı saptanmıştır.

Morphological and molecular identification of a new species Meloidogyne artiellia (Franklin) on wheat fauna in Turkey

Root-knot nematodes (Meloidogyne spp.) are among some of the most economically important plant parasitic nematodes in the world. It is known that the nematode belong to Meloidogyne genus causes significantly crop losses in vegetables and cereals. The root-knot nematodes, M. artiellia causes damages especially cereals, legumes and cruciferous crops. Isolates of M. artiellia recovered from root and soil samples collected from wheat fields in 2012 growing season were firstly identified by using morphological and molecular aspects in Hatay province of Turkey. M. artiellia was morphologically identified by using morphological features and allometric criteria of second stage juvenile and female. Also, it was molecularly identified by using the sequences of partial mitochondrial DNA cytochrome C oxidase subunit (COI) gene region and clearly separated from other species.

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