Hibrit entomopatojen nematod Heterorhabditis bacteriophora ırklarının ebeveynlerine göre etkinliklerinin karşılaştırılması

Yüksek biyolojik mücadele potansiyeline sahip olan Heterorhabditis bacteriophora Poinar, 1976 (Rhabditida: Heterorhabditidae) türünün hedef alınan zararlı böcek üzerindeki etkinliği farklı iklim özelliklerine sahip olan bölgelerde değişiklik göstermektedir. Bu çalışmada; Antalya (H.b. 6), Çanakkale (H.b. 876), Kırklareli (H.b. 17), İzmir (H.b. HIZ), Şanlıurfa (H.b. HSU) ve Adana (H.b. 10) illerinden izole edilmiş olan 6 farklı H. bacteriophora izolatı kullanılmıştır. Bu izolatların etkinlikleri Galleria mellonella (Lepidoptera: Pyralidae)’ nın son dönem larvaları üzerinde 6 farklı uygulama dozunda belirlenmiştir. En düşük etkinlik değerine sahip olan izolatın erkek ve dişi bireyleri ile denemelerde kullanılan diğer tüm ırkların erkek ve dişi bireyleri in vitro ortam koşullarında hibritlenerek 10 farklı ırk elde edilmiştir. Hibridizasyon sonucunda elde edilen ırkların G. mellonella larvaları üzerindeki etkinlikleri ebeveyn ırklar ile aynı şekilde belirlenmiştir. Çalışma sonunda ebeveyn ve hibrit ırklara ait olan etkinlik verileri karşılaştırılmış ve elde edilen 10 yeni hibrit ırkın %70’ inin ebeveynlerine göre yüksek, %30’ unun ise ebeveynlerine göre daha düşük değerde etkinlik gösterdiği tespit edilmiştir. Ayrıca hibrit ırkların böcek üzerindeki etkinlik değeri üzerine erkek ve dişi bireylerin farklı etkiler gösterdiği de tespit edilmiştir. Ebeveynlerine göre yüksek etkinlik değerine sahip olan hibrit ırkların etkinlik değerlerinin %57.1’ inin erkek, %42.9’unun ise dişi bireylerin etkisi altında kaldığı bulunmuştur

Anahtar Kelimeler:

Heterorhabditis bacteriophora, entomopatojen nematod, etkinlik, hibridizasyon, in vitro

Comparison of virulence of hybridized entomopathogenic nematode Heterorhabditis bacteriophora (Rhabditida: Heterorhabditidae) strains and their parents

Efficacy of Heterorhabditis bacteriophora Poinar, 1976 (Rhabditida: Heterorhabditidae) species can be changed on targeted insect pests in different climatic zone. In this study, six different H. bacteriophora strains, which isolated in different cities [Antalya (H.b. 6), Çanakkale (H.b. 876), Kırklareli (H.b. 17), İzmir (H.b. HIZ) and Adana (H.b.10)] from Turkey, were hybridized and their virulence were determined on the last instar of Galleria mellonella (Lepidoptera: Pyralidae) at six different dosages. Male and female individuals of having lowest virulence were crossbred with other strain females and males in vitro conditions. Ten different strains were obtained by hybridization and their virulence was compared with their parent and each other on the last instar larvae of G. melonella. The results showed that 70% of hybridized strains were more effective than their parents. The results indicated that male and female individuals have different effect on the virulence of the hybridized strains. The hybridized strains, which were more effective than their parents, were influenced from female with 42.9% and from male with 57.1%

Keywords:

Heterorhabditis bacteriophora, entomopathogenic nematodes, virulence, hybridization, in vitro,

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