Çay ekstraktlarının entomopatojen fungus sporları üzerindeki UV koruyucu özellikleri ve Galleria mellonella (L., 1758) (Lepidoptera: Pyralidae) üzerindeki öldürücü etkileri

Bu çalışma, entomopatojenik funguslar için çeşitli çay ekstraktlarının ultraviyole (UV) koruması etkinliğini araştırmayı amaçlamaktadır. UV radyasyonu, böcek zararlılarına karşı önemli biyokontrol etmenleri olan bu fungusların canlılığı üzerinde olumsuz etkilere sahip olabilir. Bu çalışma 2023 yılında Karadeniz Teknik Üniversitesi Biyoloji Bölümü Mikrobiyoloji Laboratuvarında yürütülmüştür. Çay ekstraktlarının entomopatojen funguslar [Beauveria bassiana (Bals.) Vuil. (Hypocreales: Cordycipitaceae) ve Metharizium flavoviride (Gams and Rozsypal 1956) (Hypocreales: Clavicipitaceae)] üzerindeki UV koruyucu özelliklerini ve Galleria mellonella (L.,1758) (Lepidoptera: Pyralidae) larvalarına karşı çay ekstraktlarının etkinliklerini değerlendirilmiştir. Bulgularımız, belirli çay özlerinin entomopatojen funguslar için önemli bir UV koruması sergilediğini göstermekte ve bu ekstraktların açık hava ortamlarında biyokontrol ajanlarının performansını artırmada potansiyel uygulamalarına işaret etmektedir. En yüksek UV-B koruması, fungus sporlarına siyah ve yeşil çay ekstraktları eklenerek elde edilmiş ve 10 günlük 120 dakikalık maruziyet sonunda sırasıyla 14.6 mm ve 14.3 mm’lik bir radyal büyüme ölçümü elde edilmiştir. Bu sonuçlar, tarımda çevre dostu zararlı yönetimi stratejilerinin geliştirilmesine katkıda bulunacaktır.

Anahtar Kelimeler:

Beauveria bassiana, biyolojik aktivite, Metharizium flavoviride, çay ekstraktları, UV koruma

The UV protectant properties of tea extracts on entomopathogenic fungus spores and their lethal effect on Galleria mellonella (L., 1758) (Lepidoptera: Pyralidae)

The present study aims to investigate the effectiveness of various tea extracts in providing ultraviolet (UV) protection for entomopathogenic fungi. UV radiation could have detrimental effects on viability of these fungi, which are important biocontrol agents against insect pests. This study was carried out in the Microbiology Laboratory of the Department of Biology Karadeniz Technical University in 2023. We evaluated the UV protective properties of various tea extracts in entomopathogenic fungi [Beauveria bassiana (Bals.) Vuil. (Hypocreales: Cordycipitaceae) and Metharizium flavoviride (Gams and Rozsypal 1956) (Hypocreales: Clavicipitaceae)] and tea extracts effectiveness against Galleria mellonella (L.,1758) (Lepidoptera: Pyralidae) larvae. Our findings demonstrate that certain tea extracts exhibit significant UV protection for entomopathogenic fungi, suggesting their potential application in improving the performance of biocontrol agents in outdoor environments. The highest UV-B protection was observed by adding black and green tea extracts to fungal spores, resulting in a radial growth measurement of 14.6 mm and 14.3 mm, respectively, at the end of 10 days of exposure for 120 minutes. These results contribute to the development of eco-friendly strategies for pest management in agriculture.

Keywords:

Beauveria bassiana, Metharizium flavoviride, UV protection, tea extracts, biological activity,

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Abudulai, M., B. M. Shepard & A. Salifu, 2001. Field evaluation on a neem (Azadirachta indica A. Juss)-based formulation Neemix® against Nezara viridula (L.) (Hemiptera: Pentatomidae) in cowpea. International Journal of Pest Management, 49 (2): 109-113.
Acheampong, M. A., M. P. Hill, S. D. Moore & C. A. Coombes, 2020. UV sensitivity of Beauveria bassiana and Metarhizium anisopliae isolates under investigation as potential biological control agents in South African citrus orchards. Fungal Biology, 124 (5): 304-310.
Ansari, M. A., S. Carpenter, E. J. Schulte, E. C. Pope & T. M. Butt, 2011. Entomopathogenic fungus as a biological control for an importent vector of livestock disease: The Culicoides biting midge. PloS ONE, 6 (1): e16108.
Biryol, S., D. Efe, A. Eski, Z. Demirbag & I. Demir, 2020. Fungal pathogens of Amphimallon solstitiale Linnaeus, 1758 (Coleoptera: Scarabaeidae). Turkish Journal of Entomology, 44 (3): 375-384.
Biryol, S., N. Araz, A. Eski, R. Akturk, Y. Aksu, B. Celik Gokturk, L. Bilgin & I. Demir, 2021. Biodiversity and pathogenicity of entomopathogenic fungi associated with the lesser spruce sawfly, Pristiphora abietina. Entomologia Experimentalis et Applicata, 169 (5): 414-423.
Braga, G. U. L., S. D. Flint, C. D. Miller, A. J. Anderson & D. W. Roberts, 2001a. Variability in response to UV-B among species and strains of Metarhizium anisopliae isolates from sites at latitudes from 61°N to 54°S. Journal of Invertebrate Pathology, 78 (2): 98-108.
Braga, G. U. L., S. D. Flint, C. D. Miller, A. J. Anderson & D. W. Roberts, 2001b. Both solar UVA and UVB radiation impair conidial culturability and delay germination in the entomopathogenic fungus Metarhizium anisopliae. Photochemistry and Photobiology, 74 (5): 734-739.
Braga, G. U. L., S. D. Flint, C. L. Messias, A. J. Anderson & D. W. Roberts, 2001c. Effects of UVB irradiance on conidia and germinants of the entomopathogenic hyphomycete Metarhizium anisopliae: A study of reciprocity and recovery. Photochemistry and Photobiology, 73 (2): 140-146.
Bridge, P. D., M. S. Clark & D. A. Pearce, 2005. A new species of Paecilomyces isolated from the Antarctic springtail Cryptopygus antarcticus. Mycotaxon, 92: 213-222.
Caffin, N., B. D’Arcy, L. Yao & G. Rintoul, 2004. Developing an Index of Quality for Australian Tea. RIRDC Publication, Canberra, Australia, 208 pp.
Couceiro, J. C., M. B. Fatoretto, C. G. B. Demetrio, N. V. Meyling & I. Delalibera, 2021. UV-B radiation tolerance and temperature-dependent activity within the entomopathogenic fungal genus Metarhizium in Brazil. Frontiers in Fungal Biology, 8 (2): 645737.
El Salamouny, S., M. Shapiro, K. S. Ling & B. M. Shepard, 2009a. Black tea and lignin as ultraviolet protectants for the beet armyworm nucleopolyhedrovirus. Journal of Entomological Science, 44 (1): 50-58.
El Salamouny, S., D. Ranwala, M. Shapiro, B. M. Shepard & R. R. Farrar, 2009b. Tea, Coffee, and Cocoa as Ultraviolet Radiation Protectants for the Beet Armyworm Nucleopolyhedrovirus. Journal of Economic Entomology, 102 (5): 1767-1773.
El-Husseini, M. M., A. D. El-Khaleil, M. A. El-aw & A. S. Kasem, 2012. Testing some natural additives as protectants for Spodoptera littoralis nucleopolyhedrovirus from sunlight inactivation. Egyptian Journal of Biological Pest Control, 22 (2): 191-196.
Fargues, J. F., M. Rougier, R. Goujet, N. Smits, C. Coustere & B. Itier, 1997. Inactivation of conidia of Paecilomyces fumosoroseus by nearultraviolet (UVB and UVA) and visible radiation. Journal of Invertebrate Pathology, 69 (1): 70-78.
Fargues, J., M. S. Goettel, N. Smits, A. Ouedraogo, C. Vidal, L. A. Lacey, C. J. Lomer & M. Rougier, 1996. Variability in susceptibility to simulated sunlight of conidia among isolates of entomopathogenic Hyphomycetes. Mycopathologia, 135 (3): 171-181.
Faria, M. R. & S. P. Wraight, 2007. Mycoinsecticides and Mycoacaricides: A comprehensive list with worldwide coverage and international classification of formulation types. Biological Control, 43 (3): 237-256.
Feng, M. G., T. J. Poprawski & G. G. Khachatourians, 1994. Production, formulation and application of the entomopathogenic fungus Beauveria bassiana for insect control: Current status. Biocontrol Science & Technology, 4 (1): 3-34.
Fernandes É. K. K., D. E. N. Rangel, A. M. L. Moraes, V. R. E. P. Bittencourt & D. W. Roberts, 2007. Variability in tolerance to UV-B radiation among Beauveria spp. isolates. Journal of Invertebrate Pathology, 96 (3): 237-243.
Fernandes, É. K. K., D. E. N. Rangel, G. U. L. Braga & D. W. Roberts, 2015. Tolerance of entomopathogenic fungi to ultraviolet radiation: a review on screening of strains and their formulation. Current Genetics, 61 (3): 427-440.
Fernández-Bravo, M., A. Flores-León, S. Calero-López, F. Gutiérrez-Sánchez, P. Valverde-García & E. Quesada-Moraga, 2017. UV-B radiation related effects on conidial inactivation and virulence against Ceratitis capitata (Wiedemann) (Diptera; Tephritidae) of phylloplane and soil Metarhizium sp. strains. Journal of Invertebrate Pathology, 148 (1): 142-151.
Gifani, A., R. Marzban, S. Khorramvatan, M. Ardjmand & A. Dezianian, 2021. Protective effect of different additives and natural polymers on the stability of Helicoverpa armigera NPV (HaNPV) against ultraviolet radiation. BioControl in Plant Protection, 8 (2): 151-158.
Henning, S. M., C. Fajardo-Lira, H. W. Lee, A. A. Youssefian, V. L. W. Go & D. Heber, 2003. Catechin content of 18 teas and a green tea extract supplement correlates with the antioxidant capacity. Nutrition and Cancer, 45 (2): 226-235.
Huang, B. F. & M. G. Feng, 2009. Comparative tolerances of various Beauveria bassiana isolates to UV-B irradiation with a description of a modeling method to assess lethal dose. Mycopathologia, 168 (3): 145-152.
Ibrahim, S. A., H. M. S. El-Banna & S. M. I. Abd el-kareem, 2019. Determination of the effect of some plant extracts as the UV-Protectant to the American bollworm, Helicoverpa armigera (Hübner) nucleopolyhedrosis virus (HaNPV) virulence under laboratory conditions. IOSR Journal of Agriculture and Veterinary Science, 12 (12): 10-14.
Ignoffo, C. M. & C. Garcia, 1978. UV-Photoinactivation of cells and spores of Bacillus thuringiensis and effects of peroxidase on inactivation. Environmental Entomology, 7 (2): 270-272.
Ignoffo, C. M. & C. Garcia, 1992. Influence of conidial color on inactivation of several entomogenous fungi (Hyphomycetes) by simulated sunlight. Environmental Entomology, 21 (4): 913-917.
Inglis, G. D., M. S. Goettel & D. L. Johnson, 1995. Influence of ultravioletlight protectants on persistence of the entomopathogenic fungus, Beauveria bassiana. Biological Control, 5 (4): 581-590.
Inglis, G. D., M. S. Goettel, T. M. Butt & H. Strasser, 2001. “Use of Hyphomycetous Fungi for Managing Insect Pests, 23-29”. In: Fungi as Biocontrol Agents: Progress, Problems and Potential (Eds. T. M. Butt, C. Jackson & N. Magan). CABI, New York, 390 pp.
Jackson, M. A., C. A. Dunlap & S. T. Jaronski, 2010. Ecological considerations in producing and formulating fungal entomopathogens for use in insect biocontrol. BioControl, 55 (1): 129-145.
Jaronski, S. T., 2009. Ecological factors in the inundative use of fungal entomopathogens. BioControl, 55: 159-185.
Kaiser, D., S. Bacher & G. Mène-Saffraneé Grabenweger, 2018. Efficiency of natural substances to protect Beauveria bassiana conidia from UV radiation. Pest Management Science, 75 (2): 556-563.
Katiyar, S. K., F. Afaq, A. Perez & H. Mukhtar, 2001. Green Tea Polyphenol (-)-Epigallocatechin-3-Gallate Treatment of Human Skin Inhibits Ultraviolet Radiation-Induced Oxidative Stress. Carcinogenesis, 22 (2): 287-294.
Maqbool, Z., S. Hussain, M. Imran, F. Mahmood, T. Shahzad, Z. Ahmed, F. Azeem & S. Muzammil, 2016. Perspectives of using fungi as bioresource for bioremediation of pesticides in the environment: A critical review. Environmental Science and Pollution Research, 23 (1): 16904-16925.
Moore, D., P. D. Bridge, P. M. Higgins, R. P. Bateman & C. Prior, 1993. Ultraviolet radiation damage to Metarhizium flavoviride conidia and the protection given by vegetable and mineral oils and chemical sunscreens. Annals of Applied Biology, 122 (3): 605-616.
Morley Davies, J., D. Moore & C. Prior, 1996. Screening of Metarhizium and Beauveria spp. conidia with exposure to simulated sunlight and a range of temperatures. Mycological Research, 100 (1): 31-38.
Nascimento, É., S. H. da Silva, E. R. Marques, D. W. Roberts & G. U. L. Braga, 2010. Quantification of cyclobutane pyrimidine dimers induced by UVB radiation in conidia of the fungi Aspergillus fumigatus, Aspergillus nidulans, Metarhizium acridum and Metarhizium robertsii. Photochemistry and Photobiology, 86 (6): 1259-1266.
Nian, X. G., Y. R. He, L. H. Lu & R. Zhao, 2015. Evaluation of alternative Plutella xylostella control by two Isaria fumosorosea conidial formulations-oil-based formulation and wettable powder-combined with Bacillus thuringiensis. Pest Management Science, 71 (12): 1675-1684.
Ortucu, S. & O. F. Algur, 2017. A laboratory assessment of two local strains of the Beauveria bassiana (Bals.) Vuill. against the Tetranychus urticae (Acari: Tetranychidae) and their potential as a mycopesticide. Journal of Pathogens, 2017 (1): 7628175.
Posadas, J. B., L. M. Angulo, J. I. Mini & R. E. Lecuona, 2012. Natural tolerance to UV-B and assessment of photoprotectants in conidia of six native isolates of Beauveria bassiana (Bals-Criv) Vuillemin. World Applied Sciences Journal, 20 (7): 1024-1030.
Püntener, W., 1981. Manual for field trials in plant protection. Agricultural Division, Ciba-Geigy Limited, 205 pp.
Shapiro, M., B. M. Shepard & R. Lopez, 2007a. Effect of spices upon the activity of the gypsy moth (Lepidoptera: Lymantriidae) nucleopolyhedrovirus. Journal of Entomological Science, 42 (1): 84-91.
Shapiro, M., B. M. Shepard & R. Lopez, 2007b. Effects of medicinal herbs on the biological activity of the gypsy moth nucleopolyhedrovirus. Journal of Entomological Science, 42 (3): 426-429.
Shapiro, M., S. El Salamouny & B. M. Shepard, 2008. Green tea extracts as ultraviolet protectants for the beet armyworm, Spodoptera exigua, nuclepolyhedrovirus. Biocontrol Science & Technology, 18 (6): 605-617.
Yusuf, N., C. Irby, S. K. Katiyar & C. A. Elmets, 2007. Photoprotective effects of green tea polyphenols. Photodermatology, Photoimmunology & Photomedicine, 23 (1): 48-56.
Zimmerman, G., 2007. Review on safety of the entomopathogenic fungi Beauveria bassiana and Beauveria brongniartii. Biocontrol Science & Technology, 17 (6): 553-596.