Mahmut Alper ALTINOK, Memiş KESDEK, Şaban KORDALI, Ayşe USANMAZ BOZHÜYÜK, Hacer Handan ALTINOK

Buğday biti Sitophilus granarius (L., 1758) (Coleoptera: Curculionidae) üzerinde bazı entomopatojen fungusların patojenisitesi

Bu çalışmada, yedi entomopatojen fungus izolatı Beauveria bassiana, Isaria farinosa (ARSEF-3580), Isaria fumosorosea (ARSEF-4501), Lecanicillium muscarium (ARSEF-5128), L. muscarium (ARSEF-972), Lecanicillium lecanii (TR38/11) ve ticari bir L. muscarium (Ve6), buğday biti, Sitophilus granarius (L., 1758) (Coleoptera: Curculionidae) erginlerine karşı laboratuvar şartlarında (25±1°C, 75±5% nisbi nem ve 14sa aydınlık:10sa karanlık) denenmiştir. Çalışma, Atatürk Üniversitesi’nde (Erzurum, Türkiye) 2018 yılında gerçekleştirilmiştir. Fungal izolatlar ergin bireylere iki farklı spor konsantrasyonunda (1x105 ve 1×107 ml-1) spreylenmiştir. Yüzde ölümler uygulamadan sonraki 2., 4., 6., 8. ve 10. günlerde kaydedilmiştir. Pozitif kontrol olarak ticari bir L. muscarium izolatı ve negatif kontrol olarak da steril su+Tween20 (0,25%) kullanılmıştır. Sonuçlar, entomopatojen fungus ile uygulanan S. granarius erginlerinde, uygulamadan sonraki 10 gün süresince ölüm oranlarının %1,01 ile %98,9 arasında değiştiğini göstermiştir. Yüksek konsantrasyon ve daha uzun maruz kalma süreleri ergin bireylerde artan patojenisite ile sonuçlanmıştır. Uygulanan suşlar arasında, 1×107 ml-1 konsantrasyonda I. fumosorosea, L. muscarium (ARFES-5128) ve L. lecanii izolatları, denemenin 10. gününde S. granarius erginlerinde sırasıyla %97,85%, %94,62 ve %93,58 kümülatif ölüm oranlarına ulaşmıştır. Bu üç izolatın biyolojik mücadele kapsamında yüksek derecede ümitvar oldukları değerlendirilmiştir.

Anahtar Kelimeler:

Entomopatojen, fungus, depolanmış ürün

Pathogenicity of some entomopathogenic fungi on wheat weevil Sitophilus granarius (L., 1758) (Coleoptera: Curculionidae)

In the present study, the toxicities of seven entomopathogenic fungal isolates, Beauveria bassiana (ARSEF-4984), Isaria farinosa (ARSEF-3580), Isaria fumosorosea (ARSEF-4501), Lecanicillium muscarium (ARSEF-5128), L. muscarium (ARSEF-972), Lecanicillium lecanii (TR38/11) and L. muscarium (Ve6), were tested against the adults of granary weevil, Sitophilus granarius (L., 1758) (Coleoptera: Curculionidae), under laboratory conditions (25±1°C, 75±5% RH and 14h light:10h dark). Studies were conducted in Atatürk University (Erzurum, Türkiye), in 2018. Fungal isolates were sprayed to adults at two different conidial concentrations (1×105 and 1×107 ml-1). Mortality percentages were observed on the 2nd, 4th, 6th, 8th, and 10th days of treatment. A commercial isolate of L. muscarium were used as positive control and sterile water+0.25% Tween 20 used as negative control. The results demonstrated that the mortality rates of S. granarius adults treated with entomopathogenic fungi ranged from 1.01% to 98.9% across 10-day period. Higher concentration and longer exposure periods resulted in increasing virulence on the adult individuals. Among the strains tested, at 1×107 ml-1 concentration, I. fumosorosea, L. muscarium (ARFES-5128) and L. lecanii isolates displayed 97.85%, 94.62% and 93.58% cumulative mortalities respectively, on S. granarius adults by the 10th day of the experiment. These three isolates are regarded as highly promising biological control agents.

Keywords:

Entomopathogen, fungi, stored grain,

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