Beauveria bassiana (Balsamo) Vuillemin (Deuteromycota: Hyphomycetes) izolatlarının spor süspansiyonu ve kültür filtratının farklı koşullarda Tetranychus urticae Koch, 1836 (Acari: Tetranychidae) üzerindeki etkinliği

Bu çalışmada, 2022 yılında Isparta Uygulamalı Bilimler Üniversitesi’nde Beauveria bassiana (Balsamo) Vuillemin (Deuteromycota: Hyphomycetes) izolatlarının Tetranychus urticae Koch, 1836 (Acari: Tetranychidae) üzerindeki etkinliği spor süspansiyonu ve kültür filtratı uygulanarak petri ve saksı denemeleri yoluyla laboratuvar ve iklim odası koşullarında belirlenmiştir. Petri denemesinde, B. bassiana'nın BIM-001 ve BY2 izolatlarının spor süspansiyonları (1x106, 1x107 ve 1x108 spor/ml) ve kültür filtratları (1X, 5X, 10X) petri içinde yaprak diskleri üzerinde bulunan ergin bireylere 3 m/s hızla 10 saniye mistleme şeklinde püskürtülmüştür. Saksı denemeleri petri denemelerindeki aynı uygulama yöntemiyle saksıdaki tek yapraklı bitkiler üzerinde gerçekleştirilmiştir. Petri ve saksı denemelerinde BIM-001 ve BY2'nin 108 spor/ml dozundaki ölüm oranları arasındaki fark (sırasıyla %64.00±4.52 ve %42.00±2.49, %58.00±2.91 ve %41.00±2.77) 7. gözlem gününde anlamlıydı. Saf kültür filtratı uygulamalarında BIM-001 ve BY2'deki ölüm oranları petri ve saksı denemelerinde sırasıyla %73.00±2.13 - 68.00±3.59 ve %60.00±3.65 - 57.00±5.17 olarak bulunmuştur. Hem petri hem de saksı denemelerinde BIM-001 ve BY2 kültür filtratı (1X) uygulamaları istatistiksel olarak anlamlı bulunmamıştır. Beauveria bassiana BIM-001 izolatı T. urticae'nin ergin dişilerine karşı hem in vivo hem de in vitro koşullarda BY2 izolatından daha etkili olmuştur. Sonuç olarak B. bassiana BIM-001 izolatının kültür filtratının iki noktalı kırmızı örümcek kontrolü için potansiyele sahip olduğu düşünülmektedir.

Anahtar Kelimeler:

Biyokontrol, kültür filtratı, entomopatojen fungus, Tetranychus urticae

Efficacy of spore suspension and culture filtrate of Beauveria bassiana (Balsamo) Vuillemin (Deuteromycota: Hyphomycetes) isolates on Tetranychus urticae Koch, 1836 (Acari: Tetranychidae) in different conditions

Abstract In this study, the effectiveness of Beauveria bassiana (Balsamo) Vuillemin (Deuteromycota: Hyphomycetes) isolates on Tetranychus urticae Koch, 1836 (Acari: Tetranychidae) was determined with Petri and pot experiments in laboratory and climate room conditions by applying spore suspension and culture filtrate in Isparta University of Applied Sciences of in 2022. In the Petri experiment, the spore suspensions (1x106, 1x107, and 1x108 spore/ml) and culture filtrates (1X, 5X, 10X) of BIM-001 and BY2 isolates of B. bassiana were sprayed in the form of mist for 10 seconds at a speed of 3 m/s onto leaf discs in Petri which included ten adult individuals. In the pot experiment, the same treatments were conducted with the same application method in the Petri experiment on single-leaf plants in pots. In the Petri and pot experiments, the difference between the mortality rates in the 108 spore/ml dose of BIM-001 and BY2 (64.00±4.52 and 42.00±2.49%, 58.00±2.91 and 41.00±2.77%, respectively) was significant on the 7th observation day. In the pure culture filtrate treatments, the mortality rates in the BIM-001 and BY2 were 73.00±2.13 - 68.00±3.59% and 60.00±3.65 -57.00±5.17% respectively in the Petri and pot experiments. BIM-001 and BY2 culture filtrate in both Petri and pot experiments (1X) applications were not statistically significant. The BIM-001 isolate of B. bassiana was more effective than the BY2 isolate treatment against adult females of T. urticae both in vivo and in vitro. In conclusion, it is thought that the culture filtrate of B. bassiana BIM-001 isolate is considered to have potential for the control of two-spotted spider mites.

Keywords:

Biocontrol, culture filtrate, entomopathogenic fungus, Tetranychus urticae,

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