Sema YILDIRIM, Yavuz Selim ŞAHIN, Alper SUSURLUK

Bazı Heterorhabditis bacteriophora (Poinar, 1976) (Rhabditida: Heterorhabditidae) ırklarının Lolium perenne L. (Poales: Poaceae) ve Galleria mellonella (L., 1758) (Lepidoptera: Pyralidae)'ya yönelimi

Entomopatojen nematodlar (EPN'ler) türlere bağlı olarak, birbirinden farklı konukçu bulma davranışlarına sahiptirler. Konukçu arama davranışlarının yanı sıra, bazı EPN'lerin topraktaki yönelimleri bitki köklerine bağlı olarak değişebilir. Bursa’da laboratuvar koşullarında 2019 yılında yapılan bu çalışmada bazı Heterorhabditis bacteriophora (Poinar, 1976) (Rhabditida: Heterorhabditidae) ırklarının İngiliz çimi Lolium perenne L. (Poales: Poaceae) köklerine ve Petek Güvesi Galleria mellonella (L., 1758) (Lepidoptera: Pyralidae) larvalarına yönelimleri araştırılmıştır. Bu amaçla, üç farklı H. bacteriophoraırkı kullanılmıştır. Bunlardan birisi hibrit tür (HBH) diğer ikisi ise hibrid türün ebeveynidir (HB1138, HB4). Çalışma, üç farklı deneysel kombinasyon ile laboratuvar koşullarında yapılmıştır. Y-Olfaktometre düzeneğinde A kombinasyonu bitki kökleri, larvalar ve kontrol; B kombinasyonu bitki kökleri, kontrol-I ve kontrol-II; C kombinasyonu ise mekanik olarak yaralanmış bitki kökleri, kontrol-I ve kontrol-II bölümlerini içermektedir. Elde edilen sonuçlar; Kombinasyon A'da bitki köklerine ve böcek larvalarına karşı en yüksek yönelimi HB1138 göstermiştir. Kombinasyon B'de bitki köklerine en yüksek yönelimi HBH ve kombinasyon C'de mekanik olarak yaralı bitki köklerine en yüksek yönelimi HB4 sergilemiştir. Bu çalışmada, aynı türe ait farklı EPN ırklarının bitki köklerine ve konukçu böceklere yönelimi değişebileceği tespit edilmiştir.

Anahtar Kelimeler:

Entomopatojen nematodlar, İngiliz çimi, yönelim

Orientation of some Heterorhabditis bacteriophora (Poinar, 1976) (Rhabditida: Heterorhabditidae) strains to Lolium perenne L. (Poales: Poaceae) and Galleria mellonella (L., 1758) (Lepidoptera: Pyralidae)

Entomopathogenic nematodes (EPNs) have different host-finding (foraging) behavior that varies from species to species. Besides their foraging behavior, the orientation of some EPNs can vary depending on plant roots. In the present study, the orientation of some Heterorhabditis bacteriophora (Poinar, 1976) (Rhabditida: Heterorhabditidae) strains to perennial ryegrass Lolium perenne L. (Poales: Poaceae) roots and greater wax moth Galleria mellonella (L., 1758) (Lepidoptera: Pyralidae) larvae were investigated. For this purpose, three different strains of H. bacteriophora were used. One of them was a hybrid strain (HBH) and the two were parents (HB1138 and HB4) of the hybrid strain. Three experimental combinations were conducted in the study conducted in 2019 under laboratory conditions in Bursa, Turkey: (A) plant roots, larvae and control; (B) plant roots, control I and control II; and (C) mechanically damaged plant roots, control-I and control-II in Y-tube olfactometers filled with moist sand. The results indicated that for combination A, most orientation to plants and larvae was seen in HB1138; for combination B, most orientation to plant roots was was seen in HBH; and for combination C, strain HB4 showed the most orientation to mechanically damaged plant roots. According to the results, each strain of the same EPN species may have a different response to plant roots and host insects.

Keywords:

Entomopathogenic nematodes, perennial ryegrass, orientation,

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Ali, J. G., H. T. Alborn & L. L. Stelinski, 2010. Subterranean herbivore-induced volatiles released by citrus roots upon feeding by Diaprepes abbreviatus recruit entomopathogenic nematodes. Journal of Chemical Ecology, 36 (4): 361-368.
Anbesse, S. & R. U. Ehlers, 2013. Heterorhabditis sp. not attracted to synthetic (E)-β-caryophyllene, a volatile emitted by roots upon feeding by corn rootworm. Journal of Applied Entomology, 137 (1-2): 88-96.
Bal, H. K. & P. S. Grewal, 2015. Lateral dispersal and foraging behavior of entomopathogenic nematodes in the absence and presence of mobile and non-mobile hosts. PloS ONE, 10 (6): e0129887.
Boemare, N. E., R. J. Akhurst & R. G. Mourant, 1993. DNA relatedness between Xenorhabdus spp. (Enterobacteriaceae), symbiotic bacteria of entomopathogenic nematodes, and a proposal to transfer Xenorhabdus luminescens to a new genus, Photorhabdus gen. nov. International Journal of Systematic and Evolutionary Microbiology, 43 (2): 249-255.
Boff, M. I. C., R. H. W. M. Van Tol & P. H. Smits, 2002. Behavioural response of Heterorhabditis megidis towards plant roots and insect larvae. Biocontrol, 47 (1): 67-83.
Boff, M. I., F. C. Zoon & P. H. Smits, 2001. Orientation of Heterorhabditis megidis to insect hosts and plant roots in a Y-tube sand olfactometer. Entomologia Experimentalis et Applicata, 98 (3): 329-337.
Campbell, J. F. & R. Gaugler, 1993. Nictation behaviour and its ecological implications in the host search strategies of entomopathogenic nematodes (Heterorhabditidae and Steinernematidae). Behaviour, 155-169.
Cobb, N. A., 1918. Estimating the Nematode Population of the Soil. Agricultural Technology Circular 1, Bureau of Plant Industry, United States Department of Agriculture, 48 pp.
Ehlers, R. U., 2001. Mass production of entomopathogenic nematodes for plant protection. Applied Microbiology and Biotechnology, 56 (5-6): 623-633.
Grewal, P. S., E. E. Lewis, R. Gaugler & J. F. Campbell, 1994. Host finding behaviour as a predictor of foraging strategy in entomopathogenic nematodes. Parasitology, 108 (2): 207-215.
Kaya, H. K. & R. Gaugler, 1993. Entomopathogenic nematodes. Annual Review of Entomology, 38 (1): 181-206.
Kaya, H. K. & S. P. Stock, 1997. “Techniques in Insect Nematology, 281-324”. In: Manual of Techniques in Insect Pathology (Ed. L. A. Lacey). Academic Press, San Diego, CA, USA, 409 pp.
Klein, B. M. Z., G. L. Wiegers & P. H. Smits, 1994. Biological control of cockchafer larvae (Melolontha melolontha) with the entomopathogenic nematode Steinernema glaseri. Mededelingen van de Faculteit der Landbouwwetenschappen, Rijksuniversiteit Gent, 59: 411-419.
Lacey, L. A. & R. Georgis, 2012. Entomopathogenic nematodes for control of insect pests above and below ground with comments on commercial production. Journal of Nematology, 44 (2): 218-225.
Laznik, Ž. & S. Trdan, 2013. An investigation on the chemotactic responses of different entomopathogenic nematode strains to mechanically damaged maize root volatile compounds. Experimental Parasitology, 134 (3): 349-355.
Lewis, E. E., R. Gaugler & R. Harrison, 1992. Entomopathogenic nematode host finding: response to host contact cues by cruise and ambush foragers. Parasitology, 105 (2): 309-315.
Lewis, E. E., R. Gaugler & R. Harrison, 1993. Response of cruiser and ambusher entomopathogenic nematodes (Steinernematidae) to host volatile cues. Canadian Journal of Zoology, 71 (4): 765-769.
Lortkipanidze, M. A., O. A. Gorgadze, G. S. Kajaia, N. G. Gratiashvili & M. A. Kuchava, 2016. Foraging behavior and virulence of some entomopathogenic nematodes. Annals of Agrarian Science, 14 (2): 99-103.
Rasmann, S., T. G. Köllner, J. Degenhardt, I. Hiltpold, S. Toepfer, U. Kuhlmann & T. C. Turlings, 2005. Recruitment of entomopathogenic nematodes by insect-damaged maize roots. Nature, 434 (7034): 732-737.
Rasmann, S. & T. C. Turlings, 2008. First insights into specificity of belowground tritrophic interactions. Oikos, 117 (3): 362-369.
Shapiro-Ilan, D. I., D. H. Gouge, S. J. Piggott & J. P. Fife, 2006. Application technology and environmental considerations for use of entomopathogenic nematodes in biological control. Biological Control, 38 (1): 124-133.
Susurluk, A., 2009. Seasonal and vertical distribution of the entomopathogenic nematodes Heterorhabditis bacteriophora (TUR-H2) and Steinernema feltiae (TUR-S3) in turf and fallow areas. Nematology, 11 (3): 321-327.
Susurluk, I. A., 2011. Potential of Steinernema feltiae (Rhabditida: Steinernematidae) as a biological control agent against the cabbage maggot Delia radicum (Diptera: Anthomyiidae) in oilseed rape. Turkish Journal of Agriculture and Forestry, 35 (4): 413-419.
Susurluk, I. A., I. O. Ünlü & I. Kepenekci, 2004. Host finding behavior of two different Turkish isolates of entomopathogenic nematode species, Heterorhabditis bacteriophora, Poinar 1976 (Rhabditida: Heterorhabditidae). Turkish Journal of Biology, 27 (4): 203-207.
Tonelli, M., M. F. G. V. Peñaflor, L. G. Leite, W. D. Silva, F. Martins & J. M. S. Bento, 2016. Attraction of entomopathogenic nematodes to sugarcane root volatiles under herbivory by a sap-sucking insect. Chemoecology, 26 (2): 59-66.
Turlings, T. C., I. Hiltpold & S. Rasmann, 2012. The importance of root-produced volatiles as foraging cues for entomopathogenic nematodes. Plant and Soil, 358 (1-2): 51-60.
Turlings, T. C. & F. Wäckers, 2004. Recruitment of predators and parasitoids by herbivore-injured plants. Advances in Insect Chemical Ecology, 2: 21-75.
Webster, J. & G. C. Cutler, 2003. Host-finding ability of three entomopathogenic nematode isolates in the presence of plant roots. Nematology, 5 (4): 601-608.
White, G. F., 1927. A method for obtaining infective nematode larvae from cultures. Science, 66: 302- 303.
Wiesner, A., 1993. Die Induktion Der Immunabwehr Eines Insekts (Galleria mellonella, Lepidoptera) Durch Synthetische Materialien Und Arteigene Haemolymphfaktoren. Verlag nicht ermittelbar, (Unpublished) Doctoral dissertation, Berlin,107 pp.