Seda BİRYOL, Davut EFE, Ardahan ESKİ, Zihni DEMİRBAĞ, İsmail DEMİR

Amphimallon solstitiale Linnaeus, 1758 (Coleoptera: Scarabaeidae)’nin fungal patojenleri

Avrupa Haziran böceği, Amphimallon solstitiale Linnaeus, 1758 (Coleoptera: Scarabaeidae) Türkiye’de ve dünyanın birçok bölgesinde önemli toprakaltı zararlılarından biridir. Entomopatojen funguslar bu zararlıların mücadelesinde kullanılabilecek önemli mikrobiyal mücadele ajanlarıdır ve bunların lokal böcek popülasyonlarından elde edilmesi tercih edilir. Çalışma 2017 yılında Karadeniz Teknik Üniversitesi, Fen Fakültesi, Mikrobiyoloji Laboratuvarında gerçekleştirilmiştir. Bu çalışmada, zararlıya karşı etkili bir mikrobiyal mücadele ajanı bulmak için zararlının fungal patojenleri araştırılmıştır. Bulaşık larvalardan fungus izolasyonu yapılmış ve morfolojik-moleküler karakterizasyonu sonucu izolatlar Metarhizium flavoviride Gams & Roszypal, (Deuteromycotina: Hyphomycetes) olarak tanımlanmıştır. Filogenetik analizler ve patojenite çalışmaları sonucunda izolatların M. flavoviride’nin farklı genotipleri olduğu belirlendi. En yüksek ölüm ise %96 ile As2 izolatında görülmüş olup, tüm izolatlar 106 konidya/ml konsantrasyonda %80’in üzerinde ölüme neden olmuştur. As2 izolatı ile doz denemeleri sonucunda, izolatın ortalama öldürücü konsantrasyonu 3.87 x 103 konidya/ml olarak belirlenmiştir. Bu çalışmalar, M. flavoviride As2 izolatının A. solstitiale’nin biyolojik mücadelesinde kullanılabilecek etkili bir mikrobiyal mücadele etmeni olduğunu göstermektedir.

Anahtar Kelimeler:

Amphimallon solstitiale, biyolojik mücadele, entomopatojen fungus, Metarhizium flavoviride

Fungal pathogens of Amphimallon solstitiale Linnaeus, 1758 (Coleoptera: Scarabaeidae)

European June beetle, Amphimallon solstitiale Linnaeus, 1758 (Coleoptera: Scarabaeidae) is one of the most important soilborne pests in many parts of Turkey and the world. Entomopathogenic fungi are important microbial control agents that can be used to control soilborne pests, and it is desirable to obtain them from local insect populations. The study was conducted at Karadeniz Technical University, Faculty of Science, Microbiology Laboratory in 2017. In this study, fungal pathogens of A. solstitiale were investigated to find an effective microbial control agent. Fungi were isolated from infected larvae and morphological-molecular characterization of the isolates showed that all isolates were Metarhizium flavoviride Gams & Roszypal (Deuteromycotina: Hyphomycetes). Using phylogenetic analysis and pathogenicity tests, the isolates were found to be different genotypes of M. flavoviride. All isolates gave more than 80% mortality at a concentration of 106 conidia/ml, with one isolate (As2) causing 96% mortality. Therefore, dose-mortality experiments were conducted with As2, and the median lethal concentration was determined to be 3.87 x 103 conidia/ml. This study demonstrated that M. flavoviride As2 is an effective microbial control agent that can be used for biological control of A. solstitiale.

Keywords:

Amphimallon solstitiale, biological control, entomopathogenic fungi, Metarhizium flavoviride,

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