Sıçanlarda REM Uyku Yoksunluğunun İskelet Kası Myostatin Düzeylerine Etkisi
Amaç: Uyku, kas metabolizması için önemli faktörlerden biridir. Uyku yoksunluğunun (UY) kas rejenerasyonunu bozduğu gösterilmiştir.
Myostatin iskelet kası hücreleri tarafından eksprese edilir ve kas büyümesini sınırlandırır. Çalışmamızın amacı, sıçanlarda akut uyku
yoksunluğunun iskelet kası myostatin düzeyleri üzerindeki etkilerini değerlendirmektir.
Gereç ve Yöntemler: Yirmi bir erkek Wistar albino sıçan (200-250g) rastgele üç gruba (n=7) ayrılmıştır: Kontrol grubu, Geniş platform
(GP) grubu ve UY grubu. REM UY, 72 saat boyunca modifiye çoklu platform yöntemi kullanılarak indüklenmiştir. Lökomotor aktivite
gruplar arasında açık alan testi (OFT) kullanılarak değerlendirilmiştir. Gastroknemius ve soleus kas dokuları alınmış ve kas dokularında
myostatin, malondialdehit (MDA), glutatyon (GSH) ve glikojen seviyeleri ölçülmüştür.
Bulgular: Bu çalışma, akut UY’nin kontrol grubuna kıyasla soleus kasında myostatin (1161±39,55) ve MDA (115,37±8,47) seviyelerinde
artışa neden olduğunu göstermiştir (sırasıyla p=0,036 ve p=0,01). OFT’de geçilen kare sayısı diğer gruplarla karşılaştırıldığında artmıştır
(56,33±22,02) (p=0,001 ve p=0,044). Gastroknemius kasında GSH (4,86±0,26) ve glikojen seviyeleri (5,13±0,21) UY grubunda azalmıştır
(sırasıyla p=0,007 ve p=0,028).
Sonuç: Bu veriler, REM uyku yoksunluğunun iskelet kasındaki myostatin seviyelerini ve oksidatif stres parametrelerini farklı kas tiplerinde
farklı miktarlarda değiştirerek kas metabolizmasını etkilediğini gösterebilir.
The Effects of REM Sleep Deprivation on Skeletal Muscle Myostatin Levels in Rats
Aim: Sleep is one of the crucial factors for muscle metabolism. It has been shown that sleep deprivation (SD) impairs muscle regeneration.
Myostatin is expressed by skeletal muscle cells and limits muscle growth. The purpose of our study was to evaluate the effects of acute
sleep deprivation on skeletal muscle myostatin levels in rats.
Material and Methods: Twenty one male Wistar albino rats (200-250g) were randomly allocated in to three groups (n=7): Control
group, Wide platform (WP) group and SD group. REM SD was induced by using the modified multiple platform method for 72
hours. The locomotor activity were evaluated among groups using open field test (OFT). Gastrocnemius and soleus muscle tissue were
harvested and the levels of myostatin, malondialdehyde (MDA), glutathione (GSH) and glycogen was measured in the muscle tissues.
Results: This study showed that acute SD caused an increase in myostatin (1161±39.55) and MDA levels (115.37±8.47) in the soleus
muscle compared to the control group (respectively p=0.036 ve p=0.01). Compared with the other groups, the number of crossing square
was increased in OFT (56.33±22.02) (p=0.001 ve p=0.044). GSH (4.86±0.26) and glycogen levels (5.13±0.21) in gastrocnemius muscle
was decresead in SD group (respectively p=0.007 ve p=0.028).
Conclusion: These data may represent that REM sleep deprivation affects muscle metabolism by changing myostatin levels and oxidative
stress parameters in skeletal muscle to different extents in different muscle types.
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