Sıçanlarda İntestinal İskemi Reperfüzyon ile Oluşan Akut Akciğer Hasarında Metforminin Koruyucu Etkileri
İntestinal iskemi/reperfüyon (İ/R) akciğeri içeren çoklu organ hasarına neden olmaktadır. Pulmoner doku hasarı ve solunum yetmezliği intestinal iskemi sonrası yaygın bir olaydır. Genel olarak serbest oksijen radikalleri (SOR) gibi oksidatif stres mediyatörlerinin İ/R ile oluşan pulmoner hasar gelişiminde rol oynadığına inanılmaktadır. Bu veriler ışığında, bu çalışmanın amacı, metformin tedavisinin sıçanlarda intestinal İ/ R ile indüklenen akut akciğer hasarını azaltıp azaltmayacağını araştırmaktır. İntestinal İ/R’nun sıçan modeli süperiör mezenterik arterin 45 dakika bağlanması sonrasında 3 saatlik reperfüzyonu ile oluşturuldu. Metformin intestinal iskemiden 5 gün önce oral olarak uygulandı (50,100 veya 200mg/kg dozunda). 40 adet Wistar Albino cinsi sıçan 5 gruba ayrıldı: sham konrol, I/R kontrol ve metfornin uygulanan gruplar. Malondialdehid(MDA) ve indirgenmiş glutatyon(GSH) seviyeleri oksidatif stresi değerlendirmek amacıyla ölçüldü. Hasarı gözleyebilmek için doku kesitleri hematoksilen eozin ile boyandı.
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