Bitki büyümesini teşvik eden kök bakterilerini kullanılarak asma uru hastalığı etmeni A grobacterium vitis' in biyolojik mücadelesi

Özet: Agrobacterium vitisin neden olduğu ur hastalığı, Türkiyeyi de içine alan dünyanınasma yetiştirilen pek çok yerinde yaygın ve yıkıcı bir hastalıktır. Bu çalışmada asma uruhastalığının biyolojik mücadele olanakları araştırılmıştır. Mersin, Adana, Osmaniye, Hatay,Gaziantep ve Kahramanmaraş illerinde bulunan bağ alanlarından bitki büyümesini teşvikeden aday kökbakterisi (PGPR) izolatları elde etmek için 39 toprak örneği alınmış olupt oprak örneklerinden 464 adet bakteri izole edilmiştir. Katı NBRIP besi yeri kullanılarak bu bakteri izolatlarından 63ünün fosforu çözdüğü belirlenmiştir. E n yüksek fosfor çözen 10izolat seçilerek saksı çalışmalarında kullanılmıştır. Saksı çalışmasında PGPR izolatları olanGa7/3- 6 (% 45), Ga10/2-5 (% 97), Os1/3-1 (% 80), OsD1/3-1 (% 98) ve HaD6/3- 1 (% 75)nın asmada ur ağırlığını önemli düzeyde azalttığı belirlenmiştir. Bu çalışma, TürkiyedeAgrobacterium vitisin PGPR ile biyolojik mücadelesi üzerine ilk çalışma niteliğindedir.

Biological control of grapewine crown gall disease agent Agrobacterium vitis by using plant growth promoting rhizobacteria

Abstract: Crown gall, caused by the bacterium Agrobacterium vitis, is a common anddevastating grape disease in many vineyards of the world, including in Turkey. In thisstudy, possibility of biological control of grapewine crown gall disease was investigated.Totally 464 putative Plant Growth Promoting Rhizobacteria (PGPR) were isolated from 39soil samples which were collected from vineyards of Mersin, Adana, Osmaniye, Hatay,Gaziantep and Kahramanmaraş provinces. Sixty- three bacterial strains solubilizedphosphorus on solid NBRIP medium. Ten PGPR strains which have solubilized phosphoruswith the highest ratio were used in pot experiments. PGPR strains, Ga7/3- 6 (45%), Ga10/2- 5 (97%), Os1/3- 1 (80%), OsD1/3- 1 (98%) ve HaD6/3- 1 (75%) , significantly reduced gallsweight on grape plants in pot experiments. This is the first study on biological control ofAgrobacterium vitis in Turkey.

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