The effect of streptomycin on survival, development, and some biochemical aspects of Drosophila melanogaster

The effect of streptomycin on survival, development, and some biochemical aspects of Drosophila melanogaster

Abstract: The effects of an aminoglycoside antibiotic, streptomycin, on survival rate, development time, and adult longevity of Drosophila melanogaster (Diptera: Drosophilidae) were examined by adding on the artificial diet at different concentrations in laboratory condition. The effect of streptomycin on malondialdehyde (MDA) and protein carbonyl (PCO) contents, superoxide dismutase (SOD), and catalase (CAT) activities in all developmental stages of D. melanogaster was also investigated. First-instar larvae were reared into diets with 600 mg/L, 1200 mg/L, and 1800 mg/L of streptomycin concentrations and were fed until the adult emergence. The highest concentration of streptomycin significantly reduced the survival rates of all development stages and the adult longevity of insect when compared to the control diet without streptomycin. Time to the adult stage was 7.73 ± 0.29 days in control, this developmental time in the highest concentration of streptomycin extended to 9.65 ± 0.65 days.Also, MDA content in the highest concentration of streptomycin significantly increased when compared to the control diet in the adult stage. Relative to the control, all concentrations of streptomycin significantly increased the PCO content. SOD activity was reduced in all stages of the insect. Similar results were obtained in CAT activity in the larval and pupal stages. Our results showed that experimented dietary streptomycin concentrations dramatically increased PCO content and caused significant alternations on SOD-CAT activity with the negative effects on the survival and development of D. melanogaster.Key words: Drosophila melanogaster, streptomycin, survival rate, development time, antioxidant enzymes

___

  • Aebi, H (1984). Catalase in vitro. Methods in Enzymology 105 121- 126.
  • Arun Kumar KM, Tambe VJ, Rehaman SK, Choudhuri BN, Thakur KD (2018). Effect of different diets on the biology of rice moth, Corcyra cephalonica (Stainton). Journal of Entomology and Zoology Studies 6 (3): 251-254.
  • Aslan N, Büyükgüzel E, Büyükgüzel K (2019). Oxidative effects of gemifloxacin on some biological traits of Drosophila melanogaster (Diptera: Drosophilidae). Environmental Entomology 48 (3): 667-673.
  • Aslanturk A, Kalender S, Uzunhisarcikli M, Kalender Y (2011). Effects of methidathion on antioxidant enzyme activities and malondialdehyde level in midgut tissues of Lymantria dispar (Lepidoptera) larvae. Journal of the Entomological Research Society 13 (3): 27-38.
  • Bailey AP, Koster G, Guillermier C, Hirst EM, MacRae JI et al. (2015). Antioxidant role for lipid droplets in a stem cell niche of Drosophila. Cell 163 (2): 340-353.
  • Bernardi EB, Haddad ML, Parra JRP (2000). Comparison of artificial diets for rearing Corcyra cephalonica (Stainton, 1865) (Lep., Pyralidae) for Trichogramma mass production. Revista Brasileira de Biologia 60 (1): 45-52.
  • Bonfini A, Liu X, Buchon N (2016). From pathogens to microbiota: how Drosophila intestinal stem cells react to gut microbes. Developmental & Comparative Immunology 64 (1): 22-38.
  • Bronskill J (1961). A cage to simplify the rearing of the greater wax moth, Galleria mellonella (Pyralidae). Journal of Lepidopterists’ Society 15 (2): 102-104.
  • Büyükgüzel E (2009). Evidence of oxidative and antioxidative responses by Galleria mellonella larvae to malathion. Journal of Economic Entomology 102 (1): 152-159.
  • Buyukguzel, E (2013). Protein oksidasyonun biyokimyasal ve moleküler mekanizması. Karaelmas Fen ve Mühendislik Dergisi 3(1): 40-51.
  • Büyükgüzel E, Kalender Y (2007). Penicillin-induced oxidative stress: effects on antioxidative response of midgut tissues in instars of Galleria mellonella. Journal of Economic Entomology 100 (5): 1533-1541.
  • Büyükgüzel E, Kalender Y (2008). Galleria mellonella (L.) survivorship, development and protein content in response to dietary antibiotics. Journal of Entomological Science: 43 (1): 27-40.
  • Büyükgüzel E, Kalender Y (2009). Exposure to streptomycin alters oxidative and antioxidative response in larval midgut tissues of Galleria mellonella. Pesticide Biochemistry and Physiology 94 (2-3): 112-118.
  • Büyükgüzel E, Kayaoğlu S (2014). Niklozamid’in Galleria mellonella L. (Lepidoptera: Pyralidae)’nın bazı biyolojik ve fizyolojik özelliklerine etkisi. Türkiye Entomoloji Dergisi 38 (1): 83-99.
  • Büyükgüzel K, İçen E (2004). Effects of gyrase inhibitors on the total protein content of Pimpla turionellae L. reared on an artificial diet. Journal of Entomological Science 39 (1): 108-116.
  • Cohen AC (2015). Insect Diets: Science and Technology. Boca Raton, FL, USA: CRC Press.
  • Çelik C, Büyükgüzel K, Büyükgüzel E (2019). The effects of oxyclozanide on survival, development and total protein of Galleria mellonella L. (Lepidoptera: Pyralidae). Journal of the Entomological Research Society 21 (1): 95-108.
  • Değer Y, Ertekin A, Değer S, Mert H (2008). Lipid peroxidation and antioxidant potential of sheep liver infected naturally with distomatosis. Türkiye Parazitoloji Dergisi 32 (1): 23-26.
  • Dettbarn WD, Mılatovıc D, Gupta RC (2006). Oxidative stress in anticholinesterase-induced excitotoxicity. In: Toxicology of Organophosphate and Carbamate Compounds. Cambridge, MA, USA: Academic Press, pp. 511-532.
  • Dimou I, Rempoulakis P, Economopoulos AP (2010). Olive fruit fly [Bactrocera (Dacus) oleae (Rossi) (Diptera: Tephritidae)] adult rearing diet without antibiotic. Journal of Applied Entomology 134 (1): 72-79.
  • Douglas AE (2018). The Drosophila model for microbiome research. Lab Animal: 47 (6): 157-164.
  • Esther E, Smit S, Beukes M, Apostolides Z, Pirk CW et al. (2015). Detoxification mechanisms of honey bees (Apis mellifera) resulting in tolerance of dietary nicotine. Scientific Reports 5 (1): 1-11.
  • Graf E, Benz G (1970). Toxicity of streptomycin and terramycin, and influence on growth and developmental time of Drosophila melanogaster. Experientia 26 (12): 1339-1341.
  • Güneş E, Büyükgüzel E (2017). Oxidative effects of boric acid on different developmental stages of Drosophila melanogaster Meigen, 1830 (Diptera: Drosophilidae). Türkiye Entomoloji Dergisi 41 (1): 3-15.
  • Heys C, Lizé A, Colinet H, Price TA, Prescott M et al. (2018). Evidence that the microbiota counteracts male outbreeding strategy by inhibiting sexual signaling in females. Frontiers in Ecology and Evolution 6 (1): 29.
  • Holzgrabe U, Nap CJ, Kunz N, Almeling S (2011). Identification and control of impurities in streptomycin sulfate by highperformance liquid chromatography coupled with mass detection and corona charged-aerosol detection. Journal of Pharmaceutical and Biomedical Analysis 56 (2): 271-279.
  • Huynh MP, Bernklau EJ, Coudron AT, Shelby KS, Bjostad LB et al. (2019). Characterization of corn root factors to improve artificial diet for Western Corn Rootworm (Coleoptera: Chrysomelidae) larvae. Journal of Insect Science 9 (2): 1-8.
  • Huynh MP, Meihls LN, Hibbard BE, Lapointe SL, Niedz RP et al. (2017). Diet improvement for western corn rootworm (Coleoptera: Chrysomelidae) larvae. PLoS One 12 (11): e0187997.
  • Hyršl P, Büyükgüzel E, Büyükgüzel K (2007). The effects of boric acid-induced oxidative stress on antioxidant enzymes and survivorship in Galleria mellonella. Archives of Insect Biochemistry and Physiology 66 (1): 23-31.
  • Jain SK, Levine SN (1995). Elevated lipid Peroxidation and vitamin E-quinone levels in heart ventricles of streptozotocin-treated diabetic rats. Free Radical Biology and Medicine 18 (2): 337- 341.
  • Kobeasy I, Abdel-Fatah M, Abd El-Salam SM, Mohamed ZEOM (2011). Biochemical studies on Plantago major L. and Cyamopsis tetragonoloba L. International Journal of Biodiversity and Conservation 3 (3): 83-91.
  • Kohanski MA, Dwyer DJ, Collins JJ (2010). How antibiotics kill bacteria: from targets to networks. Nature Reviews Microbiology 8 (6): 423-435.
  • Kohanski MA, Dwyer DJ, Hayete B, Lawrence CA, Collins JJ (2007). A common mechanism of cellular death induced by bactericidal antibiotics. Cell 130 (5): 797-810.
  • Krams IA, Kecko S, Jõers P, Trakimas G, Elferts D et al. (2017). Microbiome symbionts and diet diversity incur costs on the immune system of insect larvae. Journal of Experimental Biology 220 (22): 4204-4212.
  • Lambeth JD, Neish AS (2014). Nox enzymes and new thinking on reactive oxygen: a double-edged sword revisited. Annual Review of Pathology: Mechanisms of Disease 9 (1): 119-145.
  • Lee KA, Kim B, Bhin J, Kim DH, You H et al. (2015). Bacterial uracil modulates Drosophila DUOX-dependent gut immunity via Hedgehog-induced signaling endosomes. Cell Host & Microbe 17 (2): 191-204.
  • Lee WJ, Brey PT (2013). How microbiomes influence metazoan development: insights from history and Drosophila modeling of gut-microbe interactions. Annual Review of Cell and Developmental Biology 29 (1): 571-592.
  • Lesch C, Goto A, Lindgren M, Bidla G, Dushay MS et al. (2007). A role for Hemolectin in coagulation and immunity in Drosophila melanogaster. Developmental & Comparative Immunology 31 (12): 1255-1263.
  • Levine RL, Williams JA, Stadtman ER, Shacter E (1994). Carbonyl assays for determination of oxidatively modified proteins. Methods in Enzymology 233: 346-357.
  • Li G, Xia X, Zhao S, Shi M, Liu F et al. (2020). The physiological and toxicological effects of antibiotics on an interspecies insect model. Chemosphere 248: 126019.
  • Li X, Rommelaere S, Kondo S, Lemaitre B (2020). Renal purge of hemolymphatic lipids prevents the accumulation of ROSinduced inflammatory oxidized lipids and protects Drosophila from tissue damage. Immunity 52 (2): 374-387.
  • Lin XL, Kang ZW, Pan QJ, Liu TX (2015). Evaluation of five antibiotics on larval gut bacterial diversity of Plutella xylostella (Lepidoptera: Plutellidae). Insect Science 22 (5): 619-628.
  • Marklund S, Marklund G (1974). Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. European Journal of Biochemistry 47: 469-474.
  • Marra A, Hanson MA, Kondo S, Erkosar B, Lemaitre B (2021). Drosophila antimicrobial peptides and lysozymes regulate gut microbiota composition and abundance. bioRxiv. doi: 10.1101/2021.03.19.436153
  • Men TT, Thanh DNV, Yamaguchi M, Suzuki T, Hattori G et al. (2016). A Drosophila model for screening antiobesity agents. BioMed Research International 2016.
  • Mistry R, Kounatidis I, Ligoxygakis P (2016). Exploring interactions between pathogens and the Drosophila gut. Developmental & Comparative Immunology 64: 3-10.
  • Nair RV, Kulye MS, Kamath SP (2018). A single semi-synthetic diet with improved antimicrobial activity for mass rearing of lepidopteran insect pests of cotton and maize. Entomologia Experimentalis et Applicata 167 (4): 377-387.
  • Raina HS, Rawal V, Singh S, Daimei G, Shakarad M et al. (2015). Elimination of Arsenophonus and decrease in the bacterial symbionts diversity by antibiotic treatment leads to increase in fitness of whitefly, Bemisia tabaci. Infection, Genetics and Evolution 32: 224-230.
  • Raymann K, Shaffer Z, Moran NA (2017). Antibiotic exposure perturbs the gut microbiota and elevates mortality in honeybees. PLoS Biology 15 (3): e2001861.
  • Rogina B, Reenan RA, Nilsen SP, Helfand SL (2000). Extended life-span conferred by cotransporter gene mutations in Drosophila. Science 290 (5499): 2137-2140.
  • Rosengaus RB, Zecher CN, Schultheis KF, Brucker RM, Bordenstein SR (2011). Disruption of the termite gut microbiota and its prolonged consequences for fitness. Applied and Environmental Microbiology 77 (13): 4303-4312.
  • Sahayaraj K, Balasubramanian R (2016). Artificial Rearing of Reduviid Predators for Pest Management. Singapore: Springer, pp. 29-56.
  • Sahoo A, Dandapat J, Samanta L (2015). Oxidative damaged products, level of hydrogen peroxide, and antioxidant protection in diapausing pupa of Tasar silk worm, Antheraea mylitta: A comparative study in two voltine groups. International Journal of Insect Science 7: 11-17.
  • Singh P, House HL (1970a). Effects of streptomycin and potassium sorbate levels in relation to nutrient levels on the larvae of Agria affinis. Journal of Economic Entomology 63 (2): 449-454.
  • Singh P, House HL (1970b). Effects of streptomycin and potassium sorbate levels in relation to nutrient levels on the larvae of Agria affinis. Journal of Economic Entomology 63 (2): 449-454.
  • Snedecor GS, Cochran WG (1989). Statistical Methods. 8th ed. Ames, IA, USA: Iowa State University Press. SPSS Inc. (1997). User’s Manual, Version 10. Chicago, IL, USA: SPSS Inc.
  • Stanley D, Kim Y (2020). Why most insects have very low proportions of C20 polyunsaturated fatty acids: the oxidative stress hypothesis. Archives of Insect Biochemistry and Physiology 103 (1): e21622.
  • Suganya T, Varman M, Masjuki HH, Renganathan S (2016). Macroalgae and microalgae as a potential source for commercial applications along with biofuels production: a biorefinery approach. Renewable and Sustainable Energy Reviews 55: 909-941.
  • Thak A, Dhammi P, Saini HS, Kaur S (2016). Effect of antibiotic on survival and development of Spodoptera litura (Lepidoptera: Noctuidae) and its gut microbial diversity. Bulletin of Entomological Research 106 (3): 387.
  • Van AH, Coenye T (2017). The role of reactive oxygen species in antibiotic-mediated killing of bacteria. Trends in Microbiology 25 (6): 456-466.
  • Wu S, Wang G, Angert ER, Wang W, Li W et al. (2012). Composition, diversity, and origin of the bacterial community in grass carp intestine. PLoS One 7 (2): e30440.
Turkish Journal of Zoology-Cover
  • ISSN: 1300-0179
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK
Sayıdaki Diğer Makaleler

Revision of common snipe, Gallinago gallinago in morphometric analysis and building the standard reference haematological values for further studies

Ahmad WAHEED, Kamran JAFAR, Muhammad Saleem KHAN, Muhammad WAJID, Shamoon ALI, Muhammad Waseem ASLAM, Shabbir AHMAD

Size of nest complexes, the size of anthills, and infrastructure development in 4 species of red wood ants (Formica rufa, F. polyctena, F. aquilonia, F. lugubris) (Hymenoptera; Formicidae)

Stanislav STUKALYUK, Alexey GILEV, Igor ANTONOV, Maksym NETSVETOV

Molecular identification of eriophyoid mites in Thrace using the 28S and COI genes

Esengül ÖZDEMİR, Emre İNAK, Evsel DENİZHAN

Analysis of amino acids, fatty acids and neurotoxins using gas chromatography-mass spectrometry in four scorpions species inhabiting New Valley Governorate, Egypt

Shimaa Mohamed SAID, Ahmad Hamed OBUID-ALLAH, Naser Abd-Elatif EL-SHIMY, Rouwaida Saadawy ALI, Mohamed Abd-Elmoez MAHBOB

The effect of streptomycin on survival, development, and some biochemical aspects of Drosophila melanogaster

Ender BÜYÜKGÜZEL, Kemal BÜYÜKGÜZEL, Volkan KELEŞ

Effect of breeds on heat-evoked gene responses in sheep adipose tissue

Dong ZHANG, Jing PAN, Huanmin ZHOU, Yu CAO

Accumulation of chromium, cadmium and arsenic in white-tailed sea-eagle feathers (Aves: Haliaeetus albicilla) from the Danube Delta Biosphere Reserve and surrounding areas (Romania)

Dana SECELEANU, Marta PERAITA, Marian TUDOR, Janos BotondKISS, Lucian-EugenBOLBOACĂ, Cristina DESPINA, Vasile ALEXE, Adrian BURADA, Liliana TEODOROF, Mihai MARINOV, Alexandru-Cătălin DOROȘENCU, Dumitru MURARIU

Male-biased in-water population of loggerhead turtle (Caretta caretta) in Dalyan, Turkey as a possible important marine turtle area in the Mediterranean

Eyup BAŞKALE, Doğan SÖZBİLEN, Yakup KASKA