Carlos CARAMELO, Enrique MARTÍNEZ-ANSEMIL

Morphological investigations of microdrile oligochaetes (Annelida, Clitellata) using scanning electron microscopy

Over the past several years, the authors of this work have investigated external structures of microdrile oligochaetes using scanning electron microscopy (SEM). Both published and still-unpublished data have revealed new structures of interest, especially those associated with mating, ciliate sense receptors, chaetae, and dorsal pores. Mating systems can be classified in 3 categories: grasping, coupling, and embracing. Ciliate sense receptors can be classified as poorly defined (unelevated), sensory buds, or papillae, and are provided with blunt cilia and/or sharp cilia. They are present along the whole body (including clitellum, budding, and regeneration zones), scattered on the prostomium, peristomium, and pygidium, arranged in transversal rows and scattered in chaetal segments. Somatic chaetae show great variability when observed by SEM. Hair chaetae are at least potentially provided with denticulations. Dorsal pores were observed in aquatic microdriles. SEM proves to be an invaluable tool to discover important structures associated with functional anatomy in microdriles.

Anahtar Kelimeler:

Key words: Functional anatomy, microdrile oligochaetes, morphology, SEM, ciliate sense receptors, mating structures, somatic chaetae, dorsal pores

Morphological investigations of microdrile oligochaetes (Annelida, Clitellata) using scanning electron microscopy

Keywords:

Key words: Functional anatomy, microdrile oligochaetes, morphology, SEM, ciliate sense receptors, mating structures, somatic chaetae, dorsal pores,

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Aros, B., Vigh-Teichmann, I., Vigh, B. and Kovacs, J. 1978. Scanning electron microscopy of the prostomium and anterior segments of the earthworm (Lumbricus terrestris, Eisenia foetida). Z. microsk-anat. Forsch., Leipzig 92: 753-769.
Bouché, M.B. 1972. Lombriciens de France. Écologie et Systématique. INRA, Paris.
Bouché, M.L., Biagianti-Risbourg, S. and Vernet, G. 1999. A light and scanning electron microscope study of the morphology of the chaetae of Tubifex tubifex in a non-polluted medium. Hydrobiologia 411: 39-44.
Brinkhurst, R.O. 1963. Taxonomical studies on the Tubifi cidae (Annelida, Oligochaeta). Akademie Verlag, Berlin.
Brinkhurst, R.O. 1984. Th e position of the Haplotaxidae in the evolution of oligochaete annelids. Hydrobiologia 115: 25-36.
Brinkhurst, R.O. and Jamieson, B.G.M. 1971. Aquatic Oligochaeta of the World. Oliver & Boyd, Edinburgh.
Caramelo, C. and Martínez-Ansemil, E. 2010. External sense receptors in microdrile oligochaetes (Annelida, Clitellata) as revealed by scanning electron microscopy: typology and patterns of distribution in the main taxonomic groups. J. Morphol. 271: 1482-1492.
Chapman, P.M. 1979. Th e prostomial pit in Bothrioneurum vejdovskyanun Stolc (Oligochaeta): a note on detail revealed by SEM. Proc. Biol. Soc. Washington 92: 812-813.
Chapman, P.M. and Brinkhurst, R.O. 1986. Setal morphology of the oligochaetes Tubifex tubifex and Ilyodrilus frantzi (capillatus) as revealed by SEM. Proc. Biol. Soc. Washington 99: 323-327.
Chapman, P.M. and Brinkhurst, R.O. 1987. Hair today, gone tomorrow: induced chaetal changes in tubifi cid oligochaetes. Hydrobiologia 155: 45-55.
Chekanovskaya, O.V. 1962. Vodnye Maloshchetinkovye Chervy Fauny SSSR [Aquatic Oligochaeta of the USSR]. Akademia Nauk SSSR Zoologicheskii Institut. Akademia Nauk SSSR Publishers, Moscow, Leningrad. [translated from Russian, published in 1981 by Amerind Publishing Co. Pvt. Ltd., New Delhi.]
Cordero Rivera, A., Andrés, J.A., Córdoba-Aguilar, A. and Utzeri, C. 2004. Postmating sexual selection: allopatric evolution of sperm competition mechanisms and genital morphology in calopterygid damselfl ies (Insect: Odonata). Evolution 58: 349- 359.
Cuadrado, S. and Martínez-Ansemil, E. 2001. External structures used during attachment and sperm transfer in tubifi cids (Annelida, Oligochaeta). Hydrobiologia 463: 107-113.
Erséus, C. 1979. Bermudrilus peniatus n. g., n. sp. (Oligochaeta, Tubifi cidae) and two new species of Adelodrilus from the North-west Atlantic. Trans. Am. Micros. Soc. 98: 418-427.
Erséus, C. 1981. Taxonomic studies of Phallodrilinae (Oligochaeta, Tubifi cidae) from the Great Barrier Reef and the Comoro Islands with descriptions of ten species and one new genus. Zoologica Scripta 10: 15-31.
Erséus, C. and Baker, H.R. 1982. New species of the gutless marine genus Inanidrulus (Oligochaeta, Tubifi cidae) from the Gulf of Mexico and Barbados. Can. J. Zool. 60: 3063-3067.
Farnesi, R.M., Marinelli, M., Tei, S. and Vagnetti, D. 1982a. Ultrastructural aspects of mechano- and chemoreceptors in Branchiobdella pentodonta (Annelida, Oligochaeta). J. Morphol. 173: 237-345.
Farnesi, R.M., Marinelli, M., Tei, S. and Vagnetti, D. 1982b. Th e body surface of Branchiobdella pentodonta Whit. (Annelida, Oligochaeta) examined by scanning microscopy. J. Morphol. 174: 17-24.
Finogenova, N.P. and Poddubnaja, T.L. 1990. One more revision of the genus Potamothrix Vejdovský et Mrázek, 1902 (Oligochaeta, Tubifi cidae). Zool. Jb. Syst. 117: 55-83.
Grimm, R. 1986. Beiträge zur systematik der afrikanischen Naididae (Oligochaeta). III. Untersuchungen zur qualitativen und quantitativen chaetotaxonomie der Naididae. Mitt. hamb. zool. Mus. Inst. 83: 101-105.
Grimm, R. 1987. Contributions towards the taxonomy of the African Naididae (Oligochaeta). IV. Zoogeographical and taxonomical considerations on African Naididae. Hydrobiologia 155: 27-37.
Grimm, R. 1988. Beiträge zur systematik der afrikanischen Naididae (Oligochaeta). VI. Naidinae (Teil 1). Mitt. hamb. zool. Mus. Inst. 85: 73-102.
Harman, W.J. and McMahan, M.L. 1975. A reevaluation of Pristina longiseta (Oligochaeta: Naididae) in North America. Proc. Biol. Soc. Washington 88: 167-178.
Jamieson, B.G.M . 2006. Non-leech Clitellata. In: Reproductive Biology and Phylogeny of Annelida (eds. G. Rouse and F. Pleijel), Science Publishers, Enfi eld (NH), pp. 235-392.
Koene, J.M., Pförtner, T. and Michiels, N.K. 2005. Piercing the partner’s skin infl uences sperm uptake in the earthworm Lumbricus terrestris. Behav. Ecol. Sociobiol. 59: 243-249.
Loden, M.S. and Harman, W.J. 1980. Ecophenotypic variation in setae of Naididae (Oligochaeta). In: Aquatic Oligochaete Biology (eds. R.O. Brinkhurst and D.G. Cook), Plenum Press, New York.
Martínez-Ansemil, E. and Giani, N. 1986. Algunos oligoquetos acuáticos de Bolivia. Oecologia aquatica 8: 107-115.
Martínez-Ansemil, E., Sambugar, B. and Giani, N. 2002. First record of Parvidrilidae (Annelida, Oligochaeta) in Europe with a description of a new species (Parvidrilus spelaeus sp. nov.) and comments on the family and its phyletic relationships. J. Zool., London 256: 495-503.
Milbrink, G. 1983. Characteristic deformities in tubifi cid inhabiting polluted bays of Lake Vänern, southern Sweden. Hydrobiologia 106: 169-184.
Moment, G.B. and Johnson, J.E. 1979. Th e structure and distribution of external sense organs in newly hatched and mature earthworms. J. Morphol. 159: 1-16.
Ohtaka, A. 1995. A new species of the genus Rhyacodrilus Bretscher (Oligochaeta, Tubifi cidae) from Japanese lakes. Zoological Science 12: 491-498.
Römbke, J. and Schmidt, M. 1990. Setal morphology of some species of terrestrial Enchytraeidae (Oligochaeta). Trans. Am. Microsc. Soc. 109: 44-51.
Römbke, J. and Schmidt, M. 1999. REM documentation of putative cuticular sense organs of enchytraeids. Newsletter on Enchytraeidae 6: 15-20.
Schmelz, R.M. 2003. Taxonomy of Fridericia (Oligochaeta, Enchytraeidae). Revision of species with morphological and biochemical methods. Abhandlungen des Naturwissenschaft lichen Vereins in Hamburg (NF) 38, Hamburg.
Smith, M.E. 1983. External sense organs of Tubifex tubifex and Limnodrilus hoff meisteri (Tubifi cidae). Freshwater Invertebr. Biol. 5: 154-158.
Smith, M.E. 1985. Setal morphology and its intraspecifi c variation in Dero digitata and Dero nivea (Oligochaeta: Naididae). Trans. Am. Microsc. Soc. 104: 45-51.
Sperber, C. 1948. A taxonomic study of the Naididae. Zoologisca Bidrag, Frand Uppsala 28: 1-296.
Stephenson, J. 1930. Th e Oligochaeta, Clarendon Press, Oxford.
Timm, T. 1979. Hydrobiology of lakes Ulemiste and Peipus-Pskov. Oligochaeta of Lake Peipus-Pskov. Hydrobiological Research 8: 116-122.
Velando, A., Eiroa, J. and Domínguez, J. 2008. Brainless but not clueless: earthworms boost their ejaculates when they detect fecund nonvirgin partners. Proc. R. Soc. London B. 275: 1067- 1072.
Welsch, U., Storch, V. and Richards, K.S. 1984. Epidermal cells. In: Biology of the Integument. Volume 1: Invertebrates (eds. J. Bereiter-Hahn, A.G. Matoltsy and K.S. Richards), Springer, Berlin, pp. 269-296.
Yáñez, E., Cuadrado, S. and Martínez-Ansemil, E. 2006. External sense organs in freshwater oligochaetes (Annelida, Clitellata) revealed by scanning electron microscopy. J. Morphol. 267: 198-207.