Morphological examination of the resting egg structure of 3 cladoceran species [Ceriodaphnia quadrangula (O. F. Müller, 1785), Daphnia longispina (O. F. Müller, 1776), and D. magna Straus, 1820]

Morphological characteristics of the resting egg, which is important to ensure the continuity of generations, were examined to understand if there were morphologically significant differences among 3 cladoceran species. Resting eggs floating on the water surface were collected by a plankton net with a mesh size of 100 mm from Mamasın Dam Lake in November 2012 and were left to dry at room temperature in the laboratory. In this study, the size, shape, and color of the ephippium and the size of the egg/eggs enclosed in the ephippium were comparatively analyzed by stereomicroscope and scanning electron microscope. It was observed that each resting egg type had its own characteristic shape and color. Results of the statistical analysis showed that there were statistically significant differences between the sizes of the ephippium and egg/eggs enclosed in the ephippium of the resting egg of each species. We concluded that the morphological features of the resting eggs of 3 species are different from each other and that these characters can be used for species identification. In addition, we described morphological features of the resting eggs of the 3 cladoceran species in detail, gave extra information about color and size differences, and contributed to improving the literature knowledge of these species with this study.

___

  • Benzie JAH (2005). The Genus Daphnia (Including Daphniopsis) (Anomopoda: Daphniidae). Ghent, Belgium: Kenobi Productions, and Leiden, the Netherlands: Backhuys Publishers.
  • Boersma M, Boriss H, Mitchell SE (2000). Maternal effects after sexual reproduction in Daphnia magna. J Plankton Res 22: 279–285.
  • Brendonck L, De Meester L (2003). Egg banks in freshwater zooplankton: evolutionary and ecological archives in the sediment. Hydrobiologia 491: 65–84.
  • Caceres CE (1998). Interspecific variation in the abundance, production, and emergence of Daphnia diapausing eggs. Ecology 79: 1699–1710.
  • Conde-Porcuna JM, Valdés FJ, Romo S, Pérez-Martínez C (2011). Ephippial and subitaneous egg abortion: relevance for an obligate parthenogenetic Daphnia population. J Limnol 70: 69–
  • Davison J (1969). Activation of the ephippial egg of Daphnia pulex. J Gen Physiol 53: 562–575.
  • Haghparast S, Shabani A, Shabanpour B, Hoseini SA (2012). Hatching requirements of Daphnia magna Straus, 1820, and Daphnia pulex Linnaeus, 1758, diapausing eggs from Iranian populations in vitro. J Agric Sci Technol 14: 811–820.
  • Jankowski T, Straile D (2003). A comparison of egg-bank and longterm plankton dynamics of two Daphnia species, D. hyalina and D. galeata: potentials and limits of reconstruction. Limnol Oceanogr 48: 1948–1955.
  • Kotov A, Forró L, Korovchinsky NM, Petrusek A (2013). World Checklist of Freshwater Cladocera Species. World Wide Web electronic publication. Available online at http://fada. biodiversity.be/group/show/17.
  • Panarelli EA, Casanova SMC, Henry R (2008). The role of resting eggs in the recovery of zooplankton community in a marginal lake of the Paranapanema River (São Paulo, Brazil), after a long drought period. Acta Limnol Bras 20: 73–88.
  • Reid VA, Carvalho GR, George DG (2000). Molecular genetic analysis of Daphnia in the English Lake District: species identity, hybridisation and resting egg banks. Freshwater Biol 44: 247–253.
  • Rother A, Pitsch M, Hulsmann S (2010). The importance of hatching from resting eggs for population dynamics and genetic composition of Daphnia in a deep reservoir. Freshwater Biol 55: 2319–2331.
  • Slusarczyk M (2001). Food threshold for diapause in Daphnia under the threat of fish predation. Ecology 82: 1089–1096.
  • Slusarczyk M, Pietrzak B (2008). To sink or float: the fate of dormant offspring is determined by maternal behaviour in Daphnia. Freshwater Biol 53: 569–576.
  • Vandekerkhove J, Declerck S, Vanhove M, Brendonck L, Jeppesen E, Conde-Porcuna JM, De Meester L (2004). Use of ephippial morphology to assess richness of anomopods: potentials and pitfalls. J Limnol 63: 75–84.
  • Vanickova I, Seda J, Petrusek A (2010). The stabilizing effect of resting egg banks of the Daphnia longispina species complex for longitudinal taxon heterogeneity in long and narrow reservoirs. Hydrobiologia 643: 85–95.