Use of spermac staining technique in the determination of acrosomal defects in cat semen

Çalışmada, Spermac boyama tekniği kullanılarak kedi spermasında saptanan akrozom ve diğer (baş, orta-kısım ve kuyruk) anormal spermatozoon tip ve oranlarının belirlenmesine çalışıldı. Aynı bakım ve beslenme şartlarında barındırılan beş erkek sokak kedisinin sperması kullanıldı. Sperma genel anestezi altında elektro-ejakülatör ile haftada bir kez beş hafta süre ile alındı. Ejakülasyon sonrası sperma 100 pl % 0,9 NaCl solüsyonu ile sulandırıldı ve morfolojik inceleme için Spermac® Boya ile boyandı. Morfolojik özellikler, akrozom, diğer (baş, orta-kısım ve kuyruk) ve toplam morfolojik bozukluk oranlan sırasıyla; % 9,20±2,55, % 9,20±3,49 ve % 18,40±4,79 olarak bulundu. Çalışmanın sonunda, spermac boyama tekniğinin kedi spermatozoasının özellikle akrozomal bölgesini detaylı bir şekilde görebilme olanağı sağladığı ve özellikle akrozom bozukluklarının belirlenmesinde yararlı olacağı kanısına varıldı.

Kedi spermasında akrozomal bozuklukların tespitinde spermac boyama tekniğininkullanılması

The types and rates of acrosome and other (head, mid-piece and tail) abnormal spermatozoon types were determined by the Spermac® staining technique. Semen from 5 stray torn cats under the same management conditions was used. Semen collection was performed under general anesthesia by electro-ejaculator once a week for 5 weeks. After ejaculation the semen was diluted by 100 ul of 0.9% NaCI solution and stained with Spermac® stain for morphological evaluation. The morphological criteria were acrosome, other (head, mid-piece and tail) and total morphological defect rates, and were 9.20 ± 2.55%, 9.20 ± 3.49% and 18.40 ± 4.79%, respectively. It was concluded at the end of the study that the Spermac® staining technique could provide a detailed observation of cat spermatozoa, especially the acrosomal region, and could be employed in the determination of acrosomal defects.

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