Murat Erdem GÜLTİKEN, Emine Ümran BOZKURT, Dinçer YILDIZ, Durmuş BOLAT

Ultrastructure of the tongue and histochemical features of the lingual salivary glands in buzzards

The macroscopic characteristics of the tongues of 8 long-legged buzzards are described and scanning electron microscopy wasused to examine the epithelial tissue of the tongue’s surface. Crossman’s modified triple staining method and hematoxylin and eosin dyewere used to determine the locations and general histological features of the lingual salivary glands. The nature of the glandular secretionswas examined by staining with PAS, AB pH 1.0, AB pH 2.5, and PAS-AB pH 1.0 and pH 2.5, and diastase enzyme digestion. PAS stainingand weak, moderate, and strong methylation procedures as well as sialidase and hyaluronidase applications were performed. We reportthe finding of focal accumulations of the anterior lingual salivary glands at the tongue’s base with numerous duct openings in this area.This finding, in conjunction with the absence of taste papillae in the tongue epithelium, suggests that the role of the tongue in buzzardsis to mix food with saliva and to move the food bolus rapidly to the back of the oral cavity. The acidic secretions of the lingual salivaryglands not only have antimicrobial effects but also facilitate the swallowing process and may influence the digestive process in the distalparts of the alimentary tract.

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1. Emura S, Okumura T, Chen H. Scanning electron microscopic study of the tongue in the peregrine falcon and common kestrel. Okajimas Folia Anatomica Japonica 2008; 85: 11-15.
2. Iwasaki S, Asami T, Chiba A. Ultrastructural study of the keratinization of the dorsal epithelium of the tongue of Middendorff ’s bean goose, Anser fabalis middendorffii (Anseres, Antidae). Anat Rec 1997; 247: 149-163.
3. Jackowiak H, Skieresz-Szewczyk K, Godynicki S, Iwasaki S, Meyer W. Functional morphology of the tongue in the domestic goose (Anser anser f. domestica). Anat Rec 2011; 294: 1574-1584.
4. Iwasaki S. Evolution of the structure and function of the vertebrate tongue. J Anat 2002; 201: 1-13.
5. El-Bakary NER. Surface morphology of the tongue of the hoopoe (Upupa epops). J Am Sci 2011; 7: 394-399.
6. Kobayashi K, Kumakura M, Yoshimura K, Inatomi M, Asami T. Fine structure of the tongue and lingual papillae of the penguin. Arch Histol Cytol 1998; 61: 37-46.
7. Ganchrow JR, Ganchrow D. Taste bud development in chickens (Gallus gallus domesticus). Anat Rec 1987; 218: 88-93.
8. Dehkordi RAF, Parchami A, Bahadoran S. Light and scanning electron microscopic study of the tongue in the zebra finch Carduelis carduelis (Aves: Passeriformes: Fringillidae). Slov Vet Res 2010; 47: 139-144.
9. Erdoğan S, Sağsöz H, Akbalik ME. Anatomical and histological structure of the tongue and histochemical characteristics of the lingual salivary glands in the Chukar partridge (Alectorischukar, Gray 1830). Brit Poultry Sci 2012; 53: 307- 315.
10. Garguilo AM, Lovrik S, Ceccarelli P, Pedini V. Histological and histochemical studies on the chicken lingual glands. Brit Poultry Sci 1991; 32: 693-702.
11. Gelis S. Chapter 14: Evaluating and treating the gastrointestinal system. In: Harrison GJ, Lightfoot TL, editors. Clinical Avian Medicine, Vol 1. USA: Palm Beach, FL, USA: Spix Publications; 2009. pp. 412-413.
12. Al-Mansour MI, Jarrar BM. Structure and secretions of the lingual salivary glands of the white-cheeked bulbul, Pycnonotus leucogenys (Pycnontidae). Saudi J Biol Sci 2004; 11: 119-126.
13. Samar ME, Avila RE, Esteban FJ, Olmedo L, Dettin L, Massone A, Pedrosa JA, Peinado MA. Histochemical and ultrastructural study of the chicken salivary palatine glands. Acta Histochem 2002; 104: 199-207.
14. Basak F, Atalgin SH, Bozkurt EU. Tongue and lingual salivary glands of the canary: SEM and histochemical study. Folia Morphol 2017; 76: 348-354.
15. Hodges RD. The Histology of the Fowl. 1st ed. London, UK: Academic Press; 1974.
16. McLelland J. Apparatus digestorius. In: Baumel JJ, editor. Handbook of Avian Anatomy: Nomina Anatomica Avium. 2nd ed. Cambridge, MA, USA: Nuttall Ornithological Club; 1993. pp. 301-321.
17. Crole MR, Soley JT. Surface morphology of the emu (Dromaius novaehollandiae) tongue. Anat Histol Embryol 2010; 39: 355- 365.
18. Taib TN, Jarrar BM. Histochemical characterization of the lingual salivary glands of the Eurasian collared dove, Streptopelia decaocta. Pak J Biol Sci 2001; 4: 1425-1428.
19. Liman N, Bayram G, Kocak M. Histological and histochemical studies on the lingual, preglottal and laryngeal salivary glands of the Japanese quail (Coturnix coturnix japonica) at the posthatching period. Anat Histol Embryol 2001; 30: 367-373.
20. Jackoviak H, Godynicki S. Light and scanning electron microscopic study of the tongue in the white tailed eagle (Haliaeetus albicilla, Accipitridae, Aves). Ann Anat 2005; 187: 251-259.
21. Samar ME, Avila RE, De Fabro SP, Centurion C. Structural and cytochemical study of salivary glands in the Magellanic penguin, Spheniscus magellanicus and the Kelp gull, Larus dominicanus. Mar Ornithol 1995; 23: 153-157.
22. Lev R, Spicer SS. Specific staining of sulphate groups with alcian blue at low pH. J Histochem Cytochem 1964; 12: 309.
23. Spicer SS, Horn RG, Leppi TJ. Histochemistry of connective tissue mucopolysaccharides. In: Wagner BM, Smith DE, editors. The Connective Tissue. Baltimore, MD, USA: William and Wilkin Co.; 1967. pp. 251-303.
24. McManus JGA, Mowry RW. Staining Methods: Histological and Histochemical. New York, NY, USA: Paul B. Hoeber Inc.; 1960. pp. 124-151.
25. Spicer SS. Correlative study of the histochemical properties of rodent acid mucopolysaccharides. J Histochem Cytochem 1960; 8: 18-35.
26. Emura S, Okumura T, Chen H. SEM studies on the connective tissue cores of the lingual papillae of the Northern goshawk (Accipiter gentilis). Acta Anat Nippon 2008; 83: 77-80.
27. Emura S, Okumura T, Chen H. Scanning electron microscopic study of the tongue in the Oriental Scops owl (Otus scops). Okajimas Folia Anatomica Japonica 2009; 86: 1-6.
28. Emura S, Chen H. Scanning electron microscopic study of the tongue in the owl (Strix uralensis). Anat Histol Embryol 2008; 37: 475-478.
29. O’Malley B. Section three: Birds. In: O’Malley B, editor. Clinical Anatomy and Physiology of Exotic Species. 2nd ed. Philadelphia, PA, USA: WB Saunders Company; 2006. pp. 95-161.