Nattawadee KANPIPIT, Challika KAEWBORISUTH, Teerapat RUNGNIRUNDORN, Nantawan PETCHARAT, Porntippa LEKCHAROENSUK

Immunogenicity of transmembrane deleted-G protein of bovine ephemeral fever virus (BEFV) expressed in insect cells

Bovine ephemeral fever (BEF) is a common febrile disease in cattle in tropical countries causing loss of milk production and reproductive performance. Recently, severe outbreaks have occurred in many countries in East Asia, the Middle East, and Southeast Asia, especially in the rainy season, while limited vaccines and diagnostic tools are available. Thus, the aim of this study was to produce a secreted, soluble form of BEFV G glycoprotein in insect cells for further applications. BEFV G gene expressing transmembrane-deleted G glycoprotein (G?TM) was constructed and cloned into the baculovirus vector. The recombinant G?TM protein was expressed in High5 insect cells and then purified by affinity chromatography. The purified G?TM reacted specifically with a convalescent bovine serum. Four 8-week-old Wistar rats were injected subcutaneously with the purified G?TM protein. Postimmunized rat serum samples also strongly reacted with the G protein by western blot and immunoperoxidase mono player assay (IPMA). These results indicated the potential use of the transmembrane-deleted G protein to develop protein based-diagnostic tools for BEFV control programs.

Keywords:

Bovine ephemeral fever virus, truncated G protein, protein expression, baculovirus expression system, diagnostic tool,

PDF

___

Karaoğlu MT, Özgünlük İ, Demir B, Özkül A, Bergu İ. Seroprevalence of culicoides-borne disease in cattle in European Turkey. J Univ Ank Vet Fac 2007; 54: 121-125.
Venter GJ, Hamblin C, Paweska JT. Determination of the oral susceptibility of South African livestock-associated biting midges, Culicoides species, to bovine ephemeral fever virus. Med Vet Entomol 2003; 17: 133-137.
Walker PJ, Byrne KA, Cybinski DH, Doolan DL, Wang Y. Proteins of bovine ephemeral fever virus. J Gen Virol 1991; 72: 67-74.
Walker PJ, Klement E. Epidemiology and control of bovine ephemeral fever. Vet Res 2015; 46: 124.
Cybinski DH, Davis SS, Zakrzewski H. Antigenic variation of the bovine ephemeral fever virus glycoprotein. Arch Virol 1992; 124: 211-224.
Uren MF, Walker PJ, Zakrzewski H, St George TD, Byrne KA. Effective vaccination of cattle using the virion G protein of bovine ephemeral fever virus as an antigen. Vaccine 1994; 12: 845-850.
Johal J, Gresty K, Kongsuwan K, Walker PJ. Antigenic characterization of bovine ephemeral fever rhabdovirus G and Gns glycoproteins expressed from recombinant baculoviruses. Arch Virol 2008; 153: 1657-1665.
Cybinski DH, Walker PJ, Byrne KA, Zakrzewski H. Mapping of antigenic sites on the bovine ephemeral fever virus glycoprotein using monoclonal antibodies. J Gen Virol 1990; 71: 2065-2072.
Kongsuwan K, Cybinski DH, Cooper J, Walker PJ. Location of neutralizing epitopes on the G protein of bovine ephemeral fever rhabdovirus. J Gen Virol 1998; 79: 2573-2581.
Davies FG, Ochien, P, Walker AR. The occurrence of ephemeral fever in Kenya, 1968–1988. Vet Microbiol 1990; 22: 129-136.
Kato T, Aizawa M, Takayoshi K, Kokuba T, Yanase T, Shirafuji H, Tsuda T, Yamakawa M. Phylogenetic relationships of the G gene sequence of bovine ephemeral fever virus isolated in Japan, Taiwan and Australia. Vet Microbiol 2009; 137: 217-223.
Trinidad L, Blasdell KR, Joubert DA, Davis SS, Melville L, Kirkland PD, Coulibaly F, Holmes EC, Walker PJ. Evolution of bovine ephemeral fever virus in the Australian episystem. J Virol 2014; 88: 1525-1535.
Nandi S, Negi BS. Bovine ephemeral fever: a review. Comp Immunol Microbiol Infect Dis 1999; 22: 81-91.
Zheng F, Lin G, Zhou J, Wang G, Cao X, Gong X, Qiu C. A reverse-transcription, loop-mediated isothermal amplification assay for detection of bovine ephemeral fever virus in the blood of infected cattle. J Virol Methods 2011; 171: 306-309.
Yazdani F, Bakhshesh M, Esmaelizad M, Sadigh ZA. Expression of G1- epitope of bovine ephemeral fever virus in E. coli : a novel candidate to develop ELISA kit. Vet Res Forum 2017; 8: 209-213.
Zakzewski H, Cybinski DH, Walker PJ. A blocking ELISA for the detection of specific antibodies to bovine ephemeral fever virus. J Immunol Methods 1992; 151: 289-297.
Zheng FY, Lin GZ, Qiu CQ, Zhou JZ, Cao XA, Gong XW. Serological detection of bovine ephemeral fever virus using an indirect ELISA based on antigenic site G1 expressed in Pichia pastoris . Vet J 2010; 185: 211-215.
Zheng FY, Lin GZ, Qiu, CQ Zhou, JZ Cao XA, Gong XW. Development and application of G1-ELISA for detection of antibodies against bovine ephemeral fever virus. Res Vet Sci 2009; 87: 211-212.
Beygi Nassiri MT, Pasandideh R, Seyfi Abad Shapouri MR. Cloning and expression of the G1 epitope of bovine ephemeral fever virus G glycoprotein in Escherichia coli . Genetics in the 3 rd Millennium 2016; 14: 4250-4255.
Zheng FY, Lin GZ, Qiu CQ, Yuan KZ, Song JY. Expression and antigenic characterization of the epitope-G1 of the bovine ephemeral fever virus glycoprotein in Pichia pastoris . Virol Sic 2007; 22: 347-352.
Zheng F, Qiu C. Phylogenetic relationships of the glycoprotein gene of bovine ephemeral fever virus isolated from mainland China, Taiwan, Japan, Turkey, Israel and Australia. Virol J 2012; 9: 268.
Abayli H, Tonbak S, Azkur AK, Bulut H. Complete genome analysis of highly pathogenic bovine ephemeral fever virus isolated in Turkey in 2012. Arch Virol 2017; 162: 3233-3238.
Aziz-Boaron O, Leibovitz K, Gelman B, Kedmi M, Klement, E. Safety, immunogenicity and duration of immunity elicited by an inactivated bovine ephemeral fever vaccine. PLoS One 2013; 8: e82217.
Srisombundit V, Tungthumniyom N, Linchongsubongkoch W, Lekcharoensuk C, Sariya L, Ramasoota P, Lekcharoensuk P. Development of an inactivated 3Cpro-3ABC (mu3ABC) ELISA to differentiate cattle infected with foot and mouth disease virus from vaccinated cattle. J Virol Methods 2013; 188: 161-167.
Lekcharoensuk P, Wiriyarat W, Petcharat N, Lekcharoensuk C, Auewarakul P, Richt JA. Cloned cDNA of A/swine/ Iowa/15/1930 internal genes as a candidate backbone for reverse genetics vaccine against influenza A viruses. Vaccine 2012; 30: 1453-1459.
Inaba Y, Kurogi H, Takahash A, Sato K, Omori T, Goto Y, Hanaki T, Yamamoto M, Kishi S, Kodama K et al. Vaccination of cattle against bovine ephemeral fever with live attenuated virus followed by killed virus. Arch Gesamte Virusforsch 1974; 44: 121-132.
Vanselow BA, Walthall JC, Abetz I. Field trials of ephemeral fever vaccines. Vet Microbiol 1995; 46: 117-130.
Aziz-Boaron O, Gleser D, Yadin H, Gelman B, Kedmi M, Galon N, Klement E. The protective effectiveness of an inactivated bovine ephemeral fever virus vaccine. Vet Microbiol 2014; 173: 1-8.
Della-Porta AJ, Snowdon WA. Experimental inactivated virus vaccine against bovine ephemeral fever II. Do neutralizing antibodies protect against infection. Vet Microbiol 1979; 4: 197-208.
Hertig C, Pye AD, Hyatt AD, Davis SS, Mc William SM, Heine HG, Walker PJ, Boyle DB. Vaccinia virus-expressed bovine ephemeral fever virus G but not G(ns) glycoprotein induces neutralizing antibodies and protects against experimental infection. J Gen Virol 1996; 77 (Pt 4): 631-640.
Ting LJ, Lee MS, Lee SH, Tsai HJ, Lee F. Relationships of bovine ephemeral fever epizootics to population immunity and virus variation. Vet Microbiol 2014; 173: 241-248.
Hsieh YC, Chen SH, Chou CC, Ting LJ, Itakura C, Wang FI. Bovine ephemeral fever in Taiwan (2001-2002). J Vet Med Sci 2005; 67: 411-416.