Sandriele Goes de Campos DEBOLETO, Fabiana Ribeiro CALDARA, Érika Rosendo de Sena GANDRA, Rodrigo Garófallo GARCIA, Gisele Aparecida FÉLIX, Paulo Henrique BRAZ, Cláudia Marie KOMIYAMA, Kleber PELÍCIA, Irenilza de Alencar NÄÄS, Maria Fernanda de Castro BURBARELLI

Thermal rearing environment effect on behavior and metabolic profile of laying hens

For the trial, 54 Bovans White laying hens were used at 95 weeks of age and were housed in groups of three birds per cage. A completely randomized design was used with a factorial scheme of treatments 2 × 3 (rations with two energy densities × three thermal environments). Corn-soybean meal based diets with similar composition, differing only in energy levels, were obtained with soybean oil inclusion (2750 kcal and 3250 kcal metabolizable energy (ME)). Climate-controlled rooms were used to mimic a thermoneutral environment (TE) (average temperature of 24.3 °C and relative humidity of 62.3%), a hot environment (HE) (average temperature of 30.2 °C and relative air humidity of 58.8%), and a cool environment (CE) (average temperature of 17.7 °C and relative humidity of 98.5%). The behavior (ethology and vocalization), physiological parameters (cloacal temperature and heat emission), and metabolic profile (serum biochemistry) of laying hens were analyzed. Laying hens’ behavior was affected by levels of ME. With higher levels of ME, hens sat more frequently and spent more time eating when they received lower levels of ME in their diets. Laying hens under heat stress, ate, stopped, and walked more frequently than the group housed in a CE. The noise ratio (dB (A)) emitted by laying hens differed according to the thermal environment. Chickens reared in hot environments vocalized more than those raised in cool environments. Diets and thermal environments influence laying hen behaviors, with thermoneutral environments presenting greater comfort as evidenced by lower vocalization. Diets with 3250 kcal of ME present greater control of body heat reflected in lower production of adrenalin and lactate serum levels (g/L). A thermal environment above a TE is more stressful to laying hens than the CE; thus, a TE is recommended to improve laying hens’ welfare.

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1. Hu JY, Hester PY, Makagon MM, Vezzoli G, Gates RS et al. Cooled perch effects on performance and well-being traits in caged White Leghorn hens. Poultry Science 2016; 95(12): 2737-2746.
2. Payne CG. Practical aspects of environmental temperature for laying hens. World’s Poultry Science Journal 1966; 22(2): 126- 139.
3. White RR, Hanigan MD. Modelling cross-species feed intake responses to thermal stress. Journal of Agricultural Science 2015; 154(1) 136-150.
4. Liu QW, Feng JH, Chao Z, Chen Y, Wei LM et al. The influences of ambient temperature and crude protein levels on performance and serum biochemical parameters in broilers. Journal of Animal Physiology and Animal Nutrition 2016; 100(2): 301-308.
5. National Research Council (NRC). Nutrient Requirements of Poultry. 9th ed. Washington, DC, USA: National Academy Press, 1994.
6. Petherick JC, Rutter SM. Quantifying motivation using a computer controlled push-door. Applied Animal Behavior Science 1990; (27):159-167.
7. Faure JM, Lagedic H. Elasticity of demand for food and sand in laying hens subjected to variable wind-speed. Applied Animal Behaviour Science 1994; (42): 49-59.
8. Cooper JJ, Appleby MC. The value of environmental resources to domestic hens: a comparison of the work-rate for food and for nests as a function of time. Animal Welfare 2003; (12): 39- 52.
9. Silva J, Santos V. Efeito do carbonato de cálcio na qualidade da casca dos ovos durante a muda forçada. Revista Brasileira de Zootecnia 2000; 29(5): 1440-1445 (in Portuguese).
10. Liu QW, Feng JH, Chao Z, Chen Y, Wei LM et al. The influences of ambient temperature and crude protein levels on performance and serum biochemical parameters in broilers. Journal of Animal Physiology and Animal Nutrition 2016; 100(2): 301-308.
11. Rostagno HS, Albino LFT, Donzele JL, Gomes PC, Oliveira, RF et al. Tabelas brasileiras para aves e suínos—Composicão de alimentos e exigências nutricionais. 3rd ver Vicosa, MG, Brazil: ed. Editora UFV; 2017(in Portuguese).
12. Thom EC. “Cooling degree: day air conditioning, heating, and ventilation”. Transactions of the American Society Heating Refrigerating and Air-Conditioning 1958; 55(1): 65-72.
13. Richards SA. The significance of changes in the temperature of the skin and body core of the chicken in the regulation of heat loss. The Journal of Physiology 1971; 216(1): 1-10.
14. SAS Institute. 2012. User’s Guide. Statistics Version 9.3 ed. SAS Inst., Inc., Cary, NC.
15. Mack LA, Felver-Gant JN, Dennis RL, Cheng HW. Genetic variations alter production and behavioral responses following heat stress in 2 strains of laying hens. Poultry Science 2013; 92(2): 285-294.
16. Nicol CJ, Caplen G,Edgar J, Browne WJ. Associations between welfare indicators and environmental choice in laying hens. Animal Behaviour 2009; 78(2): 413-424.
17. Pereira EM, Nääs IA, Garcia RG. Identification of acoustic parameters for broiler welfare estimate. EngenhariaAgrícola 2014; 4(3): 413-421.
18. Exadaktylos V, Silva M, Berckmans D. Automatic Identification and Interpretation of Animal Sounds, Application to Livestock Production Optimisation. In: Glotin H (editor). Soundscape Semiotic, Localization. 1st ed. Croatia; 2014. p. 65-81.
19. Mcgrath N, Dunlop R, Dwyer C, Burman O, Phillips CJC. Hens vary their vocal repertoire and structure when anticipating different types of reward. Animal Behaviour 2017; 130(1): 79- 96.
20. Elwinger K, Fisher C, Jeroch H, Sauveur B, Tiller H et al. A brief history of poultry nutrition over the last hundred years. Worlds Poultry Science Journal 2016; 72(4): 1-20.
21. Classen HL. Diet energy and feed intake in chickens. Animal Feed Science Technology 2017; 233(1): 13-21.
22. Tancharoenrat P, Ravindran V, Zaefarian F, Ravindran G. Influence of age on the apparent metabolisable energy and total tract apparent fat digestibility of different fat sources for broiler chickens. Animal Feed Science and Technology 2013; 186 (3- 4): 186-192.
23. Chowdhury VS, Tomonaga S, Nishimura S, Tabata S, Cockrem, JF et al. Hypothalamic gonadotropin-inhibitory hormone precursor mRNA is increased during depressed food intake in heat-exposed chicks. Comparative Biochemistry Physiology - Part A: Molecular & Integrative Physiology; 2012; 162(3): 227- 233.
24. Mueller-Hennessen M, Sigl J, FuhrmannJC, Witt H, Reszka R et al. Metabolic profiles in heart failure due to nonischemiccardiomyopathy at rest and under exercise. ESC Heart Failure Journal 2017; 4(2): 178-189.