The effects of the L. plantarum strain RC1.4 starter culture with or without sucrose addition on fermentation efficacy, microbial content, and aerobic stability indicators of alfalfa silage

The effects of the L. plantarum strain RC1.4 starter culture with or without sucrose addition on fermentation efficacy, microbial content, and aerobic stability indicators of alfalfa silage

To develop the approach for efficient alfalfa silage fermentation, L. plantarum strain RC1.4 (LP-RC1.4) was used as starter culture with or without sucrose. Trial groups consisted of ten repetitions for each treatment including (1) control, with added sterile water (10 mL); (2) LP, silages treated with LP-RC1.4 alone (106 cfu g–1 FM); (3) LP-S1 , silages treated with LP-RC1.4 (106 cfu g–1 FM) plus sucrose at 10 g/kg fresh matter; (4) LP-S2 , silages treated with LP-RC1.4 (106 cfu g–1 FM) plus sucrose at 20 g kg–1 fresh matter. Inoculation improved the quality of the silaging demonstrating a lower decrease of silage dry matter (DM) in comparison to the control silage. The CO2 production was also lower in the inoculated silages. The addition of sucrose improved microbiological and biochemical parameters of the resulting alfalfa silage, simultaneously with the increased number of lactobacilli, raised the content of water-soluble carbohydrates, while the number of yeasts and molds decreased. It was concluded that LP-RC1.4 inoculation should be supported with sucrose to insure domination of LAB fermentation and enhance of the silage quality of alfalfa.

___

  • 1. Agarussi MCN, Rosinea OBP, de Paula RA, da Silva VP, Roseira JPS et al. Novel lactic acid bacteria strains as inoculants on alfalfa silage fermentation. Science Report 2019; 9: 8007. doi:10.1038/s41598-019-44520-9
  • 2. Pahlow G, Muck RE, Driehuis F, Elferink SJWHO, Spoelstra SF. Microbiology of ensiling. In Buxton, DR, Muck, RE, Harrison JH (Editors) Silage Science and Technology. Madison, WI: Agron. Monogr. ASA, CSSA and SSSA; 2003. pp. 31–94.
  • 3. Muck RE. Recent advances in silage microbiology. Agriculture and Food Science 2013; 22: 3-15.
  • 4. Fabiszewska AU, Zielińska KJ, Wróbel B. Trends in designing microbial silage quality by biotechnological methods using lactic acid bacteria inoculants: a minireview World Journal of Microbiology and Biotechnology 2019; 35: 76. doi:10.1007/ s11274-019-2649-2.
  • 5. Oliveira AS, Weinberg ZG, Ogunade IM, Cervantes AAP, Arriola KG et al. Meta- analysis of effects of inoculation with homofermentative and facultative heterofermentative lactic acid bacteria on silage fermentation, aerobic stability, and the performance of dairy cows. Journal of Dairy Science 2017; 100 (6): 4587-4603. doi:10.3168/jds.2016-11815
  • 6. Madigan MT, Martinko JM, Bender KS, Buckley DH, Stahl DA. Brock Biology of Microorganisms. 14th ed. San Francisco, USA: Benjamin-Cummings Publishing Company; 2015.
  • 7. Avila CLS, Carvalho, BF. Silage fermentation—updates focusing on the performance of micro-organisms. Journal of Applied Microbiology 2019; 128: 966-984. doi:10.1111/jam.14450
  • 8. Zhang Q, Yu Z, Wang X. Isolating and evaluating lactic acid bacteria strains with or without sucrose for effectiveness of silage fermentation. Grassland Science 2015; 61: 167-176. doi: 10.1111/grs.12097
  • 9. Rongrong Li, Jiang D, Zheng M, Tian P, Zheng M et al. Microbial community dynamics during alfalfa silage with or without clostridial fermentation. Science Report 2020; 10: 17782. doi: 10.1038/s41598-020-74958
  • 10. Ozduven ML, Cam AC. The effects of bacterial inoculants and/or enzymes on the fermentation characteristics and aerobic stability of alfalfa ensiled at different stages of maturity. International Journal of Current Research 2017; 9: 45983- 45988.
  • 11. Liu Q, Dong Z, Shao T. Effect of additives on fatty acid profile of high moisture alfalfa silage during ensiling and after exposure to air. Animal Feed Science and Technology 2018; 236: 29-38. doi:10.1016/j.anifeedsci.2017.11.022
  • 12. Zielińska K, Fabiszewska A, Stefańska I. Different aspects of Lactobacillus inoculants on the improvement of quality and safety of alfalfa silage. Chilean Journal of Agricultural Research 2015; 75: 298-306. doi:10.4067/S0718 -58392 01500 04000 05
  • 13. Guo XS, Ke WC, Ding WR, Ding LM, Xu DM et al. Profiling of metabolome and bacterial community dynamics in ensiled Medicago sativa inoculated without or with Lactobacillus plantarum or Lactobacillus buchneri. Science Reports 2018; 8: 357-366. doi:10.1038/s41598-017-18348-0
  • 14. Louws FJ, Fulbright DW, Stephens CT, DeBruijn FJ. Specific genomic fingerprints of phytopathogenic Xanthomonas and Pseudomonas pathovars and strains generated with repetitive sequences and PCR. Applied Enviromental Microbiology 1994; 60: 2286-2295.
  • 15. De S, Kaur G, Roy A, Dogra G, Kaushik R et al. A Simple method for the efficient isolation of genomic DNA from lactobacilli isolated from traditional Indian Fermented Milk (dahi). Indian Journal of Microbiology 2010; 50 (4): 412-418. doi: 10.1007/s12088-011-0079-4
  • 16. Weisburg WG, Barns SM, Pelletier DA, Lane DJ. 16S ribosomal DNA amplification for phylogenetic study. Journal of Bacteriology 1991; 173 (2): 697-703. doi: 10.1128/jb.173.2.697- 703.1991
  • 17. Altschul SF, Gish W, Miller W, Meyers EW, Lipman DJ. Basic local alignment search tool. Journal of Molecular Biology 1990; 215: 403-410.
  • 18. Broderick GA, Kang JH. Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media. Journal of Dairy Science 1980; 63: 64- 75. doi:10.3168/jds.S0022-0302(80)82888-8
  • 19. Ashbell G, Weinberg ZG, Azrieli A, Hen Y, Horev B. A simple system to study the aerobic deterioration of silages. Canadian Agricultural Engineering 1991; 34: 171-175.
  • 20. Ministry of Agriculture, Fisheries and Food. The Analysis of Agricultural Material. Reference Book, London, UK: H.M. Stationery Office,1986. pp. 427-428.
  • 21. AOAC. Official Methods of Analysis, 15th ed., Association of Official Analytical Chemists, Arlington, VA, USA: The Association, 1990.
  • 22. Van Soest PJ, Robertson JB, Lewis BA. Methods of dietary fiber, neutral detergent fiber and non-starch polysaccharides in relation to animal nutrition. Journal of Dairy Science 1991; 74: 3583-3597. doi: 10.3168/jds.S0022-0302(91)78551-2
  • 23. Seale D. Bacterial inoculants as silage additives. Journal of Applied Bacteriology 1986; 61: 9-26.
  • 24. SAS, SAS User’s Guide: Statistics, 5th ed. Cary, NC, USA: SAS Institute Inc., 1989.
  • 25. Steel RGD, Torrie JH, Dickey DA. Principles and Procedures of Statistics: A Biometrical Approach. 3rd ed. New York, NY, USA: Mc Graw Hill Book Company, 1997.26. McDonald P, Henderson N, Heron S. The Biochemistry of Silage. 2nd ed. Marlow England: Chalcombe Publications; 1991.
  • 27. Mahanna B. Proper management assures high-quality silages, grains. Feedstuffs 1994; 12-15.
  • 28. Rodrigues PHM, De Almeida LFS, Lucci CS, Melotti L, De Lima, FR. Efeitos da adição de inoculantes microbianos sobre o perfil fermentativo da silagem de alfafa adicionada de polpa cítrica. Revista Brasileira de Zootecnia 2004; 33: 1646-1653.
  • 29. Muck RE. Inoculant of silage and its effects on silage quality. In: Informational Conference with Dairy and Forage Industries. US Dairy Forage Research, Madison, WI; 1996. pp. 43–52.
  • 30. Muck RE, Nadeau EMG, McAllister TA, Contreras-Govea FE, Santos MC, Kung Jr L. Silage review: Recent advances and future uses of silage additives. Journal of Dairy Science 2018; 101: 3980-4000. doi.org/10.3168/jds.2017-13839
  • 31. Denek N, Can A, Avcı M, Aksu T, Durmaz H. The effect of molasses-based pre-fermented juice on the fermentation quality of first-cut lucerne silage. Grass and Forage Science 2011; 66: 243–250. doi: 10.1111/j.1365-2494.2011.00783.x
  • 32. Zheng ML, Niu DZ, Jiang D, Zuo SS, Xu CC. Dynamics of microbial community during ensiling direct-cut alfalfa with and without LAB inoculant and sugar. Journal of Applied Microbiology 2017; 122: 1456-1470. doi:10.1111/jam.13456
  • 33. Kung L, Shaver RD, Grant RJ, Schmidt RJ. Silage review: Interpretation of chemical, microbial, and organoleptic components of silages. Journal of Dairy Science 2018; 101: 4020-4033. doi.org/10.3168/jds.2017-13909
  • 34. Harper KJ, McNeill DM. The role iNDF in the regulation of feed intake and the importance of its assessment in subtropical ruminant systems (the role of iNDF in the regulation of forage intake). Agriculture 2015; 5: 778-790. doi:10.3390/ agriculture5030778
  • 35. Owens FN, Sapienza DA, Hassen A. Effect of nutrient composition of feeds on digestibility of organic matter by cattle: A review. Journal of Animal Science 2010; 88: 151-69. doi: 10.2527/jas.2009-2559
  • 36. Aksu T, Baytok E, Bolat D. Effects of a bacterial silage inoculant on corn silage fermentation and nutrient digestibility. Small Ruminant Research 2004; 55: 249-252.
  • 37. Baytok E, Aksu T, Karslı MA, Muruz H. The Effects of formic acid molasses, and inoculant as silage additives on corn silage composition and ruminal fermentation characteristics in sheep. Turkish Journal of Veterinary Animal Science 2005; 29: 469-479.
  • 38. Muck RE. Silage microbiology and its control through additives. Revista Brasileira de Zootecnia 2010; 39: 183-191
  • 39. Denek N, Can A, Avcı M, Aksu T. The Effect of fresh and frozen pre-fermented juice on the fermentation quality of alfalfa silage. Kafkas Universitesi Veteriner Fakultesi Dergisi 2012; 18 (5): 785-790, doi:10.9775/kvfd.2012.6396
  • 40. Filya I, Muck RE, Contreas-govea FE. Inoculant effects on alfalfa silage: Fermentation products and nutritive value. Journal of Dairy Science 2007; 90:5108-5114. doi: 10.3168/jds.2006-877
  • 41. Filya I. The effect of Lactobacillus buchneri and Lactobacillus plantarum on the fermentation, aerobic stability, and ruminal degradability of low dry matter corn and sorghum silages. Journal of Dairy Science 2003; 86: 3575-3581. doi: 10.3168/jds.S0022-0302(03)73963-0
  • 42. Lima R, Lourenco M, Diaz RF, Castro A, Fievez V. Effect of combined ensiling of sorghum and soybean with or without molasses and lactobacilli on silage quality and in vitro rumen fermentation. Animal Feed Science and Technology 2010; 155: 122-131. doi: 10.1016/j.anifeedsci.2009.10.008
  • 43. Heinritz SN, Martens SD, Avila P, Hoedtke S. The effect of inoculant and sucrose addition on the silage quality of tropical forage legumes with varying ensilability. Animal Feed Science and Technology 2012; 174: 201-210. doi:10.1016/j. anifeedsci.2012.03.017
  • 44. Zhang T, Li L, Wang X, Zeng Z, Hu Y et al. Effects of Lactobacillus buchneri and Lactobacillus plantarum on fermentation, aerobic stability, bacteria diversity and ruminal degradability of alfalfa silage. World Journal of Microbiology and Biotechnology 2009; 25: 965-971. doi:10.1007/s11274-009-9973-x
  • 45. Muck RE, Kung LJ. Effects of silage additives on ensiling. In: Proceeding from the Silage: Field to Feedbunk North American Conference; Hershey, USA; 1997. pp. 187-199.
  • 46. Moon NJ. Inhibition of the growth of acid tolerant yeasts by acetate, lactate and propionate and their synergistic mixtures. Journal of Applied Bacteriology 1983; 55: 454-460. doi: 10.1111/j.1365-2672.1983.tb01685.x
Turkish Journal of Veterinary and Animal Sciences-Cover
  • ISSN: 1300-0128
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK
Sayıdaki Diğer Makaleler

The effect of rangeland quality on the mohair quality of Angora goats fed on the natural rangelands

Betül Zehra SARIÇİÇEK

Effects of using processed barley and supplemented multi-enzymes in laying hen rations on egg production, egg quality, and egg fatty acids

Yavuz GÜRBÜZ, Osman ÖZYÜRÜR

Variations in the median sacral crest and angulation of the first sacral spinous process associated with sacrocaudal fusion in greyhounds

Sa’ad, M. Y. ISMAIL, Christina M. MURRAY, Mark A. STEVENSON, Hung-Hsun YEN, Helen, M. S. DAVIES

An application of least square support vector machine model with parameters optimization for predicting body weight of Harnai sheep breed

Muhammad ALI, Farhat IQBAL, Abdul RAZIQ, Zil E HUMA

Cheyletiella yasguri Smiley, 1965 (Acarina: Cheyletiellidae) infestations in six puppies in Kocaeli province of Turkey, and successful treatment with selamectin

Onur CEYLAN, Önder ÖZTÜRK

Evaluation of some morphological and genetic characteristics of Çatalburun dog breed

Fatma Tülin ÖZBAŞER, Fatih ATASOY, Banu YÜCEER ÖZKUL, Metin ERDOĞAN, Bora ÖZARSLAN

Guava extract as an antioxidant additive in diets of Japanese breeder quails to mitigate the effect of egg storage time on newly hatched quality

Nadja Susana Mogyca LEANDRO, Hyara Paula Fleuri XAVIER, Itallo Conrado Sousa de ARAÚJO, Helder Freitas de OLIVEIRA, Alessandra Gimenez MASCARENHAS, Emmanuel ARNHOLD, Billy Noronha MARQUES, Heloisa Helena de Carvalho MELLO

Mediator margins in the dairy supply chain and factors influencing marketing preferences in Eastern Mediterranean region of Turkey

Mehmet Saltuk ARIKAN, Mehmet Ferit CAN, Aytekin GÜNLÜ, Yılmaz ARAL, Tuğba SARIHAN ŞAHİN

The effects of the L. plantarum strain RC1.4 starter culture with or without sucrose addition on fermentation efficacy, microbial content, and aerobic stability indicators of alfalfa silage

Mehtap GÜNEY, Murat DEMİREL, Sibel ERDOĞAN, Taylan AKSU, Shamil VALİDOV, Liliia KARİMOVA, Taner LEVENDOĞLU

Technical staffs’ knowledge and attitudes survey regarding tail docking on dairy farms of Turkey

Mahmut KALİBER, Çağrı Çağlar SİNMEZ, İsmail ÜLGER, Ali YİĞİT, Gökhan ASLIM