Katarzyna ZABEK, Stanislaw MILEWSKI, Roman WOJCIK, Andrzej Krzysztof SIWICKI

The effects of supplementing diets fed to pregnant and lactating ewes with Saccharomyces cerevisiae dried yeast

The objective of this study was to determine the effect of supplementing diets fed to ewes (in late pregnancy and during lactation or only during lactation) with Saccharomyces cerevisiae dried brewer’s yeast on the yield and chemical composition of milk and on selected parameters of humoral and cellular immunity. The investigated indicators were analyzed on days 28 and 70 of lactation. The addition of dried yeast contributed to a significant increase in milk yield, and the obtained milk was characterized by a lower somatic cell count, suggesting improved mammary gland health. Diet supplementation had a beneficial influence on the health status of ewes, leading to increased nonspecific humoral and cellular immune responses in ewes, including gamma globulin concentrations, lysozyme and ceruloplasmin activity levels, respiratory burst activity and potential killing activity of phagocytes, and the proliferative response of mitogen-stimulated T cells and B cells.

Anahtar Kelimeler:

Key words: Sheep, Saccharomyces cerevisiae, milk performance, humoral and cellular immunity

The effects of supplementing diets fed to pregnant and lactating ewes with Saccharomyces cerevisiae dried yeast

Keywords:

Key words: Sheep, Saccharomyces cerevisiae, milk performance, humoral and cellular immunity,

PDF

___

Grela ER, Semeniuk V. Consequences of the withdrawal of antibiotic growth promoters from animal feeding. Med Wet 2006; 5: 502–507.
Dobicki A, Preś J, Zachwieja A, Mordak R, Jakus W. Influence of yeast preparations on chosen biochemical blood parameters and the composition of cow milk. Med Wet 2007; 63: 955–959.
Gopalakannan A, Arul V. Enhancement of the innate immune system and disease-resistant activity in Cyprinus carpio by oral administration of β-glucan and whole cell yeast. Aquac Res 2010; 41: 884–892.
Li P, Gatlin D M. Evaluation of brewers yeast (Saccharomyces cerevisiae) as a feed supplement for hybrid striped bass (Morone chrysops × M. saxatilis). Aquaculture 2003; 219: 681–692.
Milewski S. Effect of yeast preparations Saccharomyces cerevisiae on meat performance traits and blood hematological indices in sucking lambs. Med Wet 2009; 65: 51–54.
Waszkiewicz-Robak B, Karwowska W. Brewer’s yeast as an ingredient enhancing immunity. Pol J Food Nutr Sci 2004; 13: 85–
Suphantharika M, Khunrae P, Thanrdkit P, Verduyn C. Preparation of spent brewer’s yeast β-glucans with a potential application as an immunostimulant for black tiger shrimp, Penaeus monodon. Biores Technol 2003; 88: 55–60.
Beauchemin KA, Krehbiel CR, Newbold CJ. Enzymes, bacterial direct-fed microbials and yeast: principles for use in ruminant nutrition. In: Mosenthin R, Zentek J, Żebrowska T, editor. Biology of Nutrition in Growing Animals. Amsterdam: Elsevier; 2006. pp. 251–284.
Li J, Xing J, Li D, Wang X, Zhao L, Lv S, Huang D. Effects of β-glucan extracted from Saccharomyces cerevisiae on humoral and cellular immunity in weaned piglets. Arch Anim Nutr 2005; 59: 303–312.
Małaczewska J, Milewski S. Immunomodulating effect of Inter Yeast S on the non-specific and specific cellular and humoral immunity in lambs. Pol J Vet Sci 2010; 13: 163–170.
Jarrige R. Ruminant Nutrition. Recommended Allowances and Feed Tables. Warsaw, Poland: Omnitech Press; 1993.
Osikowski M, Porębska W, Korman K. Feeding Standards for Sheep. In: Ryś R, editor. Standards for Cattle and Sheep Feeding by the Conventional System. 12th ed. Krakow: Institute of Zootechnology; 1998.
AOAC. Official Methods of Analysis of the Association of Official Analytical Chemists. 15th ed. Gaithersburg, MD, USA: AOAC; 1990.
Parry RM, Chandau RC, Shahani RM. A rapid and sensitive assay of muramidase. Proc Soc Exp Biol Med 1965; 119: 384– 3
Siwicki AK, Anderson DP. Immunostimulation in Fish: Measuring the Effects of Stimulants by Serological and Immunological Methods. Washington, DC, USA: United States Fish and Wildlife Service; 1993.
Siwicki AK, Studnicka M. Ceruloplasmin activity in carp (Cyprinus carpio). Bamidgeh 1986; 38, 126–129.
Lowry OH, Rosebrough NJ, Farr AL, Randall R. Protein measurements with the Folin phenol reagent. J Biol Chem 1951; 193: 265–275.
Chung S, Secombes SJ. Analysis of events occurring within teleost macrophages during the respiratory burst. Comp Biochem Physiol 1988; 89B: 539–544.
Siwicki AK, Krzyżanowski J, Bartoszcze M, Mizak B, Paluch S, Szmigielski S, Jeljaszewicz J, Pulverer G. Adjuvant properties of killed Propionibacterium avidum KP-40 vaccination of dogs against canine parvovirosis. Deutsche Tierarztliche Wochenschrift 1998; 105: 173–203.
Rook GAW, Steele J, Umar J, Dockrel HMA. Simple method for the solubilization of reduced NBT and its use as a colorimetric assay for activation of human macrophages by gamma interferon. J Immunol Meth 1995; 82: 161–167.
Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Meth 1983; 65: 55–63.
Milewski S, Sobiech P. Effect of dietary supplementation with Saccharomyces cerevisiae dried yeast on milk field, blood biochemical and hematological indices in ewes. Bull Vet Inst Puławy 2009; 53: 753–758.
Dobicki A, Preś J, Zachwieja A, Kwaśnicki R. Saccharomyces cerevisiae preparations in the feeding of cows and their effect on milk yield and composition as well as rumen microorganisms. EJPAU 2006; 9: 48.
Robinson PH, Garrett JE. Effect of yeast culture (Saccharomyces cerevisiae) on adaptation of cows to postpartum diets and on lactational performance. J Anim Sci 1999; 77: 988–999.
Swartz DL, Muller LD, Rogers GW, Varga GA. Effect of yeast cultures on performance of lactating dairy cows: a field study. J Dairy Sci 1994; 77: 3073–3080.
Olechowicz J, Steppa R. Effect of health status on the quality of mammary glands of sheep’s milk. Rocz Nauk Zoot Supl 2000; 6: 269–273.
Wójcik R, Milewski S, Małaczewska J, Tański Z, Brzostowski H, Siwicki AK. Defence mechanisms of the offspring of ewes fed a diet supplemented with yeast (Saccharomyces cerevisiae) during pregnancy and lactation. Centr Eur J Immunol 2008; 33: 197–201.
Milewski S, Sobiech P, Bednarek D, Wójcik R, Małaczewska J, Zaleska B, Siwicki AK. Effect of oligosaccharides supplementation on the meat performance traits and selected indicators of humoral immunity in lambs. Bull Vet Inst Puławy 2010; 54: 175–179.
Wójcik R. Effect of brewers’ yeast (Saccharomyces cerevisiae) extract on selected parameters of humoral and cellular immunity in lambs. Bull Vet Inst Puławy 2010; 54: 181–187.