The results of consecutive superovulations in cows by induction with various exogenous progesterone routes
The aim of this study was to determine the possibility of yielding more transferable embryos from cows superovulated with/without exogenous progesterone (ear implant and intravaginal device). Two experiments were conducted in Holstein Friesian cows to evaluate the effect of exogenous progesterone on the yield of corpus luteum (CL), total embryo and ova, and transferable embryos before superovulation. A superovulation program using 2 different progesterone resources was applied (G1 = intravaginal device, G2 = ear implant). Superovulation and uterus irrigations were done at 28-day intervals in the treatment groups. Cows in the control group were superovulated at 60-day intervals without application of exogenous progesterone. The mean numbers of CLs and transferable embryos for the G1, G2, and control groups were, respectively 4.82 ± 0.29, 6.71 ± 0.29, and 7.81 ± 0.31 for CLs and 0.86 ± 0.35, 3.50 ± 0.35, and 1.53 ± 0.39 for transferable embryos. It was shown that exogenous manipulation of the estrus cycle by progesterone could be applied without superovulation intervals in Holstein cows. It may also be postulated that the ear implant was more effective than the intravaginal device as a progesterone source in the superovulation program.
The results of consecutive superovulations in cows by induction with various exogenous progesterone routes
The aim of this study was to determine the possibility of yielding more transferable embryos from cows superovulated with/without exogenous progesterone (ear implant and intravaginal device). Two experiments were conducted in Holstein Friesian cows to evaluate the effect of exogenous progesterone on the yield of corpus luteum (CL), total embryo and ova, and transferable embryos before superovulation. A superovulation program using 2 different progesterone resources was applied (G1 = intravaginal device, G2 = ear implant). Superovulation and uterus irrigations were done at 28-day intervals in the treatment groups. Cows in the control group were superovulated at 60-day intervals without application of exogenous progesterone. The mean numbers of CLs and transferable embryos for the G1, G2, and control groups were, respectively 4.82 ± 0.29, 6.71 ± 0.29, and 7.81 ± 0.31 for CLs and 0.86 ± 0.35, 3.50 ± 0.35, and 1.53 ± 0.39 for transferable embryos. It was shown that exogenous manipulation of the estrus cycle by progesterone could be applied without superovulation intervals in Holstein cows. It may also be postulated that the ear implant was more effective than the intravaginal device as a progesterone source in the superovulation program.
___
- Donaldson LE. Embryo production in superovulated cows: transferable embryos correlated with total embryos. Theriogenology 1984; 21: 517–525.
- Mapletoft RJ, Steward KB, Adams GP. Recent advances in the superovulation in cattle. Reprod Nutr Dev 2002; 42: 601–611.
- Velazquez MA. The role of nutritional supplementation on the outcome of superovulation in cattle. Anim Reprod Sci 2011; 126: 1–
- Mapletoft RJ, Martinez MF, Colazo MG, Kastelic JP. The use of controlled internal drug release devices fort the regulation of bovine reproduction. J Anim Sci 2003; 81: E28–E36.
- Callejas S, Alberio R, Cabodevila J, Aller J, Catalano R, Teruel M, Dulout F. Effect of progesterone administration on the ovarian response to superovulatory treatments in cattle. Anim Reprod Sci 2008; 107: 9–19.
- Diaz T, Pancarci SM, Drost M, Schmitt EJ, Ambrose JD, Fredrikson WE, Tatcher WW. Effects of the persistent dominant follicle on the ability of follicle stimulating hormone to induce follicle development and ovulatory responses. J Dairy Sci 2001; 84: 88–89.
- Guilbault LA, Grasso F, Lussier JG, Rouillier P, Matton P. Decreased superovulatory responses in heifers superovulated in the presence of a dominant follicle. J Reprod Fertil 1991; 91: 81–89.
- Akyol N, Kizil SH, Tuncer PB. Study of superovulation and embryo transfer in cattle. Lalahan Hay Ars Enst Derg 2004; 44: 1–
- Zizlavsky J, Riha J, Urban F, Machal L, Stipkova M. Production of embryos from repeated superovulations of cows during one calving interval. Czech J Anim Sci 2002; 47: 92–97.
- Callesen H, Liboriussen T, Greve T. Practical aspects of multiple ovulation-embryo transfer in cattle. Anim Reprod Sci 1996; 42: 215–226.
- Curtis JL. Cattle embryo transfer procedure. San Diego, CA, USA: Academic Press Inc.; 1991.
- Kastelic JP, McCartney DH, Olson WO, Barth AD, Garcia A, Mapletoft RJ. Estrus synchronization in cattle using estradiol, melengestrol acetate and PGF. Theriogenology 1996; 46: 1295– 130
- Martínez MF, Kastelic JP, Adams GP, Cook B, Olson WO, Mapletoft RJ. The use of progestins in regimens for fixed-time artificial insemination in beef cattle. Theriogenology 2002; 57: 1049–1059.
- Goulding D, Williams DH, Roche JF, Boland MP. Effect of exogenous progesterone on superovulatory response in heifers inseminated with fresh or frozen semen. J Reprod Fertil 1994; 100: 505–510.
- Boland MP, Goulding D, Roche JF. Alternative gonadotrophins for superovulation in cattle. Theriogenology 1991; 35: 5–17.
- Bo GA, Guerrero DC, Adams GP. Alternative approaches to setting up donor cows for superstimulation. Theriogenology 2008; 69: 81–87.
- Sawyer GJ, Broadbent PJ, Dolman DF. Ultrasound monitored ovarian responses in normal and superovulated cattle given exogenous progesterone at different stages of the oestrus cycle. Anim Reprod Sci 1995; 38: 187–201.
- Kanagawa H, Shimohira I, Saitoh N. Manual of Bovine Embryo Transfer. Tokyo, Japan: Livestock Technology Association; 1995.
- Kojima T. Embryo Transfer (ET) in Cattle-Theory and Practice. Shirakawa, Japan: National Livestock Breeding Center Press; 1999.
- Hasler JF. The Holstein cow in embryo transfer today as compared to 20 years ago. Theriogenology 2006; 65: 4–16.
- Bo GA, Tribulo H, Caccia M, Tribulo R. Superovulatory response of beef heifers treated with estradiol benzoate, progesterone and CIDR-B vaginal devices. Theriogenology 1998; 49: 375.
- Sartori R, Souza AH, Guenther JN, Caraviello DZ, Geiger LN, Schenk JL, Wiltbank MC. Fertilization rate and embryo quality in superovulated Holstein heifers artificially inseminated with X-sorted or unsorted sperm. Anim Reprod 2004; 1: 86–90.
- Bülbül B, Kırbaş M, Köse M, Dursun Ş. Investigation of superovulation response in Brown Swiss cows after synchronization using progesterone and oestradiol valerate. Kafkas Univ Vet Fak Derg 2010; 16: 463–468.
- Hasler JF, McCauley AD, Schermerhorn EC, Foote RH. Superovulatory responses of Holstein cows. Theriogenology 1983; 19: 83–99.
- Mori J. Advances in Farm Animal Embryo Transfer Hormone Research. Shirakawa, Japan: National Livestock Breeding Center Press; 2001.
- Oikawa T, Numabe T, Kikuchi T, Watanabe G, Taya K. Repeated induction program of superovulation in cattle throughout one-year using a progesterone device and porcine (p) FSH. Theriogenology 1998; 49: 382.
- Santos JEP, Cerri RLA, Sartori R. Nutritional management of the donor cow. Theriogenology 2008; 69: 88–97.
- Kojima T, Soma T, Oguri N. The effects of simplification of donor preparation, several superovulatory treatments by FSH-P and the single insemination regimen on embryo production in Japanese beef cattle. Jap Zootech Sci 1989; 60: 721–727.