Identification and Antimicrobial Susceptibility of Staphylococcus aureus and Coagulase Negative Staphylococci from Bovine Mastitis in the Aydın Region of Turkey

Three hundred bovine milk samples with mastitis were used. Eighty-five (28.33%) strains were identified as Staphylococcus aureus whereas 60 (20.00%) strains were isolated as coagulase negative staphylococcus. Bacteriological cultures of coagulase negative staphylococcus were identified as 20 (33.33%) strains of S. hyicus, 16 (26.66%) strains of S. chromogenes, 9 (15.00%) strains of S. epidermidis, 5 (8.33%) strains of S. haemolyticus, 4 (6.66%) strains of S. sciuri, 3 (5.00%) strains of S. lentis and 3 (5.00%) strains of S. cohnii subsp. cohnii. The antibiotic susceptibility test results showed that Staphylococcus aureus strains were susceptible to ciprofloxacin (100.00%), neomycin + bacitracin + tetracycline (100.00%), kanamycin (85.00%), cefquinome (85.00%) and amoxycillin + clavulanic acid (84.00%), while they were resistant to penicillin (95.00%) and oxacillin (60.00%). Beside this, it has been found that the coagulase negative staphylococcus strains were susceptible to cefquinome (100.00%) and neomycin + bacitracin + tetracycline (100.00%), but resistant to penicillin (90.00%) and oxacillin (73.00%).

Anahtar Kelimeler:

Mastitis, Staphylococcus aureus, coagulase negative staphylococcus identification, antimicrobial susceptibility testing

Identification and Antimicrobial Susceptibility of Staphylococcus aureus and Coagulase Negative Staphylococci from Bovine Mastitis in the Aydın Region of Turkey

Keywords:

Mastitis, Staphylococcus aureus, coagulase negative staphylococcus identification, antimicrobial susceptibility testing,

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Leslie, K.E., Schukken, Y.H.: Herd Programs for Elimination and Preventing Staphylococcus aureus Mastitis. In: National Mastitis Council Annual Meeting, Kansas City, Arlington, 1993; 36-47. 2. Guidry, A., Fattom, A., Atul, P., O’Brien, C., Shepherd, S., Lohuis, J.: Serotyping scheme for Staphylococcus aureus isolated from cows with mastitis. Am. J. Vet. Res., 1998; 59: 1537-1539.
Zecconi, A., Piccinini, R.: Culturing and Identifying Staph. aureus in Dairies: How Many Staph. aureus Do We Have? In: National Mastitis Council Annual Meeting, Arlington, 1998; 48-57.
Craven, N., Anderson, J.C.: Phagocytosis of Staphylococcus aureus by bovine mammary gland macrophages and intracellular protection from antimicrobial action in vitro and in vivo. J. Dairy Sci., 1984; 51: 513-523. 5. Chaffer, M., Leitner G., Winkler M., Saran A.: Coagulase negative stahpylococcus intermedius isolated from milk from dairy cows in Israel. Vet. Rec., 1998; 143: 592-593.
Jorun, J.: Classification of coagulase negative staphylococci
isolated from bovine clinical and subclinical mastitis. Vet. Microbiol., 1991; 27: 151-158. 7. Timms, L.L., Schultz L.H.: Dynamics and significance of coagulase negative staphylococcal intramammary infections. J. Dairy Sci., 1987; 70: 2648-2657.
Matsunaga, T., Kamata S., Kakiichi N., Uchida K.: Characteristics of Staphylococcus aureus isolated from peracute, acute and chronic bovine mastitis. J. Vet. Med. Sci., 1993; 55: 297-300.
Karadzhov, I., Mekhandzhiiska L., Gerganova E.: Coagulase negative staphylococci isolated from the milk of cows with subclinical mastitis. Vet. Med. Nauki., 1981; 18: 51-54.
McDougall, S.: Efficacy of two antibiotic treatments in curing clinical and subclinical mastitis in lactating dairy cows. New Zeal. Vet. J., 1998; 46: 226-232.
Oliver, S.P., Jayaro B.M.: Coagulase negative staphylococcal intramammary infections in cows and heifers during the nonlactating and periparturient periods. J. Vet. Med. B, 1997; 44: 355-363.
McDonald, J.S., Anderson, A.J.: Antibiotic sensitivity of Staphylococcus aureus and coagulase negative staphylococci isolated from infected bovine mammary glands. Cornell Vet., 1981; 71: 391.
Eliopoulis, G.M.: Penicillins. In: The Antimicrobial Agents Annual 1. Peterson, P.K. and Verhoef, J. Eds. Elsevier Sci. Publ., New York, 1986; pp. 144.
Arda, M., Minbay, A., Leloğlu, N., Aydın, N., Kahraman, M., Akay, Ö., Ilgaz, A., İzgür, M., Diker, K.S.: Özel Mikrobiyoloji. Streptokok mastitisleri. Medisan Yayın Serisi No: 26, Ankara, 1997: 37.
Holt, J.G., Krieg, N.R., Sneath, P.H.A., Stanley, J.T., Williams T.S.: Bergey’s Manual of Determinative Bacteriology. 9thEdition. William and Wilkins, Baltimore, Maryland, USA., 1994; 532.
Koneman, E.W., Allen, S.D., Janda, W.M, Schreckenberger, P.C., Winn, W.C. Jr.: Color Atlas and Textbook of Diagnostic Microbiology. 5th Philadelphia., 1997; 539-566.
Bauer, A.U., Kirby, W.M., Sherris, J.C, Tarck, M.: Antibiotic succeptibility testing by a standardized single disc method. J. Clin Pathol., 1966; 45: 493-494.
Radostits, O.M., Blood, D.C., Gay, C.C., Hinchcliff, K.W.: Mastitis. In: Veterinary Medicine. A Textbook of the Diseases of Cattle, Sheep, Pigs, Goats and Horses., 9th ed. W.B. Saunders, London., 2000; 603-700.
Jarp, J.: Classification of coagulase negative staphylococci isolated from bovine clinical and subclinical mastitis. Vet. Microbiol., 1991; 27: 151-158.
Nickerson, S.C., Owens W.E., Boddie R.L.: Mastitis in dairy heifers: Initial studies on prevalence and control. J. Dairy Sci., 1995; 78: 1607-1618.
Trinidad, P., Nickerson S.C., Alley T.K.: Prevalence of intramammary infection and teat canal colonization in unbred and primigravid dairy heifers. J. Dairy Sci., 1990; 73: 107-114.
Kaya, O., Erganifl, O., Kuyucuoğlu, Y.: Cow mastitis, caused by microorganisms and their susceptibities to antibiotics. Türk Vet. Hek. Derg., 1993; 5: 49-50.
Akar, F., Kaya, O., Kırkan, fi.: Studies on the identification and antibiotic resistance of the microorganisms which caused mastitis in dairy cows in Aydın region. Bornova Vet. Kontr. Araflt. Enst. Md. Derg., 1998; 23: 97-102.
Kaya, O., Kırkan, fi., Gülal, M., Ünal, B.: Determined and antibiotic susceptibility of microorganisms which caused clinic mastitis in dairy cows in Aydın region. Pendik Vet. Mikrobiol. Derg., 1999; 30: 25-29.
Erganifl, O., Kuyucuoğlu, Y., Ok, Ü.: Biotyping of coagulase negative and positive staphylococci caused to sheep and cows mastitis. Veterinarium, 1995; 6: 23-27.
Werckenthin, C., Cardoso, M., Martel, J.L., Schwarz, S.: Antimicrobial resistance in staphylococci from animals with particular reference to bovine Staphylococcus aureus, porcine Staphylococcus hyicus, and canine Staphylococcus intermedius. Vet. Res., 2001; 32: 341-362.
Myllys, V., Asplund K., Brofeld E., Hirvela-Koski V., Honkanen- Buzalski T., Junttila J., Kulkas I., Myllykangas O., Niskanen M., Saloniemi H., Sandholm M., Saranpaa T.: Bovine mastitis in Finland in 1988 and 1995 - Changes in prevalence and antimicrobial resistance. Acta Vet. Scand., 1998; 39: 119-126.
Aarestrup, F.M., Wegener H.C., Rosdahl V.T., Jensen N.E.: Staphylococcal and other bacterial species associated intramammary infections in Danish dairy herds. Acta Vet. Scand., 1995; 36: 475-487.
Aarestrup, F.M., Jensen, N.E.: Development of penicillin resistance among Staphylococcus aureus isolated from bovine mastitis in Denmark and other countries. Microb. Drug Resist., 1998; 4: 247-256.
Watts, J.L., Yancey, R.J.Jr.: Identification of veterinary pathogens by use of commercial identification systems and new trends in antimicrobial susceptibility testing of veterinary pathogens. Clin. Microbiol. Rev., 1994; 7: 3346.