The Effects of BSA and FCS on In Vitro Maturation of Bitch Oocytes

The effects of protein sources added to the medium for maturation of bitch oocytes were investigated. Ovaries from spayed bitches were transported to the laboratory in PBS at 38 ºC within an hour. Recovered oocytes were divided into three groups and left for 72 h maturation at 38.5 ºC under an atmosphere of 5% CO2 and high humidity. Group 1 (Control): TCM 199 medium, Group 2 (BSA): TCM 199 medium supplemented with 0.3% BSA, Group 3 (FCS): TCM 199 medium supplemented with 5% FCS. At the end of the maturation period, all oocytes were denuded from cumulus cells by pipetting and fixed in 1:3 acetic acid: ethanol solution for 48 h. The oocytes were then stained with 2% aceto-orcein and examined under a phased-contrast microscope at x400 magnification for maturation criteria. It was observed that 16.58% (34/205), 37.08% (79/213) and 22.81% (47/206) of the oocytes resumed meiosis in the control, BSA and FCS groups, respectively; a total number of 624 oocytes were used. In the BSA group, the rate of oocytes that resumed meiosis was significant (p < 0.05) compared to the FCS and control groups. This rate was also significant (p < 0.05) in the FCS group compared to the control group. According to these results, it can be suggested that addition of protein sources to the medium for in vitro maturation of bitch oocytes is necessarily important, and BSA gives better results than FCS does.

The Effects of BSA and FCS on In Vitro Maturation of Bitch Oocytes

The effects of protein sources added to the medium for maturation of bitch oocytes were investigated. Ovaries from spayed bitches were transported to the laboratory in PBS at 38 ºC within an hour. Recovered oocytes were divided into three groups and left for 72 h maturation at 38.5 ºC under an atmosphere of 5% CO2 and high humidity. Group 1 (Control): TCM 199 medium, Group 2 (BSA): TCM 199 medium supplemented with 0.3% BSA, Group 3 (FCS): TCM 199 medium supplemented with 5% FCS. At the end of the maturation period, all oocytes were denuded from cumulus cells by pipetting and fixed in 1:3 acetic acid: ethanol solution for 48 h. The oocytes were then stained with 2% aceto-orcein and examined under a phased-contrast microscope at x400 magnification for maturation criteria. It was observed that 16.58% (34/205), 37.08% (79/213) and 22.81% (47/206) of the oocytes resumed meiosis in the control, BSA and FCS groups, respectively; a total number of 624 oocytes were used. In the BSA group, the rate of oocytes that resumed meiosis was significant (p < 0.05) compared to the FCS and control groups. This rate was also significant (p < 0.05) in the FCS group compared to the control group. According to these results, it can be suggested that addition of protein sources to the medium for in vitro maturation of bitch oocytes is necessarily important, and BSA gives better results than FCS does.

Kaynak Göster

Bibtex @ { tbtkveterinary151844, journal = {Turkish Journal of Veterinary and Animal Sciences}, issn = {1300-0128}, eissn = {1303-6181}, address = {}, publisher = {TÜBİTAK}, year = {2002}, volume = {26}, pages = {1453 - 1458}, doi = {}, title = {The Effects of BSA and FCS on In Vitro Maturation of Bitch Oocytes}, key = {cite}, author = {Evecen, Mithat and Baran, Alper and Alkan, Serhat and Özdaş, Ö. Banu} }
APA Evecen, M , Baran, A , Alkan, S , Özdaş, Ö . (2002). The Effects of BSA and FCS on In Vitro Maturation of Bitch Oocytes . Turkish Journal of Veterinary and Animal Sciences , 26 (6) , 1453-1458 .
MLA Evecen, M , Baran, A , Alkan, S , Özdaş, Ö . "The Effects of BSA and FCS on In Vitro Maturation of Bitch Oocytes" . Turkish Journal of Veterinary and Animal Sciences 26 (2002 ): 1453-1458 <
Chicago Evecen, M , Baran, A , Alkan, S , Özdaş, Ö . "The Effects of BSA and FCS on In Vitro Maturation of Bitch Oocytes". Turkish Journal of Veterinary and Animal Sciences 26 (2002 ): 1453-1458
RIS TY - JOUR T1 - The Effects of BSA and FCS on In Vitro Maturation of Bitch Oocytes AU - Mithat Evecen , Alper Baran , Serhat Alkan , Ö. Banu Özdaş Y1 - 2002 PY - 2002 N1 - DO - T2 - Turkish Journal of Veterinary and Animal Sciences JF - Journal JO - JOR SP - 1453 EP - 1458 VL - 26 IS - 6 SN - 1300-0128-1303-6181 M3 - UR - Y2 - 2021 ER -
EndNote %0 Turkish Journal of Veterinary and Animal Sciences The Effects of BSA and FCS on In Vitro Maturation of Bitch Oocytes %A Mithat Evecen , Alper Baran , Serhat Alkan , Ö. Banu Özdaş %T The Effects of BSA and FCS on In Vitro Maturation of Bitch Oocytes %D 2002 %J Turkish Journal of Veterinary and Animal Sciences %P 1300-0128-1303-6181 %V 26 %N 6 %R %U
ISNAD Evecen, Mithat , Baran, Alper , Alkan, Serhat , Özdaş, Ö. Banu . "The Effects of BSA and FCS on In Vitro Maturation of Bitch Oocytes". Turkish Journal of Veterinary and Animal Sciences 26 / 6 (Haziran 2002): 1453-1458 .
AMA Evecen M , Baran A , Alkan S , Özdaş Ö . The Effects of BSA and FCS on In Vitro Maturation of Bitch Oocytes. Turkish Journal of Veterinary and Animal Sciences. 2002; 26(6): 1453-1458.
Vancouver Evecen M , Baran A , Alkan S , Özdaş Ö . The Effects of BSA and FCS on In Vitro Maturation of Bitch Oocytes. Turkish Journal of Veterinary and Animal Sciences. 2002; 26(6): 1453-1458.
IEEE M. Evecen , A. Baran , S. Alkan ve Ö. Özdaş , "The Effects of BSA and FCS on In Vitro Maturation of Bitch Oocytes", Turkish Journal of Veterinary and Animal Sciences, c. 26, sayı. 6, ss. 1453-1458, Haz. 2002
  • ISSN: 1300-0128
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK

23.3b 29.6b

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