Detection of Cattle Infected with Theileria annulata in Fields by Nested PCR, IFAT and Microscopic Examination of Blood Smears

The aim of this study was to diagnose Theileria annulata infected cattle by microscopy, IFAT and nested PCR and to compare the sensitivity and specificity of these diagnostic methods. A total of 147 blood and sera samples were collected from healthy cattle in four localities of Polatlı district, Ankara, where tropical theileriosis is prevalent. Examination results revealed positivity rates of 31.3%, 44.9% and 61.2% for microscopy, IFAT and nested PCR, respectively. It was shown that nested PCR is more sensitive and specific when compared to microscopical examination and serological findings. It was also shown that nested PCR is more sensitive than single step PCR. This study, which is the first work utilising nested PCR in the detection of T. annulata in Turkey, showed that nested PCR is more sensitive and specific when compared to microscopy and IFAT and can be used in further epidemiological studies.

Detection of Cattle Infected with Theileria annulata in Fields by Nested PCR, IFAT and Microscopic Examination of Blood Smears

The aim of this study was to diagnose Theileria annulata infected cattle by microscopy, IFAT and nested PCR and to compare the sensitivity and specificity of these diagnostic methods. A total of 147 blood and sera samples were collected from healthy cattle in four localities of Polatlı district, Ankara, where tropical theileriosis is prevalent. Examination results revealed positivity rates of 31.3%, 44.9% and 61.2% for microscopy, IFAT and nested PCR, respectively. It was shown that nested PCR is more sensitive and specific when compared to microscopical examination and serological findings. It was also shown that nested PCR is more sensitive than single step PCR. This study, which is the first work utilising nested PCR in the detection of T. annulata in Turkey, showed that nested PCR is more sensitive and specific when compared to microscopy and IFAT and can be used in further epidemiological studies.

Kaynak Göster

Bibtex @ { tbtkveterinary151846, journal = {Turkish Journal of Veterinary and Animal Sciences}, issn = {1300-0128}, eissn = {1303-6181}, address = {}, publisher = {TÜBİTAK}, year = {2002}, volume = {26}, pages = {1465 - 1469}, doi = {}, title = {Detection of Cattle Infected with Theileria annulata in Fields by Nested PCR, IFAT and Microscopic Examination of Blood Smears}, key = {cite}, author = {Vatansever, Zati and Nalbantoğlu, Serpil} }
APA Vatansever, Z , Nalbantoğlu, S . (2002). Detection of Cattle Infected with Theileria annulata in Fields by Nested PCR, IFAT and Microscopic Examination of Blood Smears . Turkish Journal of Veterinary and Animal Sciences , 26 (6) , 1465-1469 .
MLA Vatansever, Z , Nalbantoğlu, S . "Detection of Cattle Infected with Theileria annulata in Fields by Nested PCR, IFAT and Microscopic Examination of Blood Smears" . Turkish Journal of Veterinary and Animal Sciences 26 (2002 ): 1465-1469 <
Chicago Vatansever, Z , Nalbantoğlu, S . "Detection of Cattle Infected with Theileria annulata in Fields by Nested PCR, IFAT and Microscopic Examination of Blood Smears". Turkish Journal of Veterinary and Animal Sciences 26 (2002 ): 1465-1469
RIS TY - JOUR T1 - Detection of Cattle Infected with Theileria annulata in Fields by Nested PCR, IFAT and Microscopic Examination of Blood Smears AU - Zati Vatansever , Serpil Nalbantoğlu Y1 - 2002 PY - 2002 N1 - DO - T2 - Turkish Journal of Veterinary and Animal Sciences JF - Journal JO - JOR SP - 1465 EP - 1469 VL - 26 IS - 6 SN - 1300-0128-1303-6181 M3 - UR - Y2 - 2021 ER -
EndNote %0 Turkish Journal of Veterinary and Animal Sciences Detection of Cattle Infected with Theileria annulata in Fields by Nested PCR, IFAT and Microscopic Examination of Blood Smears %A Zati Vatansever , Serpil Nalbantoğlu %T Detection of Cattle Infected with Theileria annulata in Fields by Nested PCR, IFAT and Microscopic Examination of Blood Smears %D 2002 %J Turkish Journal of Veterinary and Animal Sciences %P 1300-0128-1303-6181 %V 26 %N 6 %R %U
ISNAD Vatansever, Zati , Nalbantoğlu, Serpil . "Detection of Cattle Infected with Theileria annulata in Fields by Nested PCR, IFAT and Microscopic Examination of Blood Smears". Turkish Journal of Veterinary and Animal Sciences 26 / 6 (Haziran 2002): 1465-1469 .
AMA Vatansever Z , Nalbantoğlu S . Detection of Cattle Infected with Theileria annulata in Fields by Nested PCR, IFAT and Microscopic Examination of Blood Smears. Turkish Journal of Veterinary and Animal Sciences. 2002; 26(6): 1465-1469.
Vancouver Vatansever Z , Nalbantoğlu S . Detection of Cattle Infected with Theileria annulata in Fields by Nested PCR, IFAT and Microscopic Examination of Blood Smears. Turkish Journal of Veterinary and Animal Sciences. 2002; 26(6): 1465-1469.
IEEE Z. Vatansever ve S. Nalbantoğlu , "Detection of Cattle Infected with Theileria annulata in Fields by Nested PCR, IFAT and Microscopic Examination of Blood Smears", Turkish Journal of Veterinary and Animal Sciences, c. 26, sayı. 6, ss. 1465-1469, Haz. 2002