Mehmet Akif YÖRÜK, Taylan AKSU, Mehmet GÜL, Duran BOLAT

The Effect of Soybean Meal Treated with Formaldehyde on Amount of Protected Protein in the Rumen and Absorption of Amino Acid from Small Intestines

This study was conducted to determine the effects of soybean meal (SBM) treated with formaldehyde on rumen fermentation pattern, microbial protein synthesis, amount of by-pass protein, and amino acid absorption from small intestines. Four 1-year-old rams with ruminal, duodenal, and ileal cannulas were fed 800 g of dry clover plus a concentrate mixture containing 100 g of SBM treated with 0%, 0.3%, 0.6%, and 0.9% formaldehyde. The amount of crude protein passed through the duodenum was not affected by the formaldehyde treatment; however, the amount of microbial protein decreased in rams fed with SBM treated with both 0.3% and 0.6% formaldehyde (P < 0.05). Digestibility of acid detergent fibre (ADF) and neutral detergent fibre (NDF) in the duodenum and ileum did not differ by treatments. In the rams fed SBM treated with both 0.3% and 0.6% formaldehyde, absorbability of arginine, histidine, leucine, methionine, phenylalanine, and valine (essential amino acids), as well as alanine and aspartic acid (non-essential amino acids) significantly increased, whereas tyrosine absorbability notably decreased (P < 0.05). Formaldehyde treatment increased the absorbability of other essential amino acids (leucine and valine) and non-essential amino acids (serine and tyrosine) in the ileum (P < 0.05). Increasing the percentage of formaldehyde linearly increased total essential amino acid absorption in the duodenum (P < 0.05). In conclusion, 0.3% and 0.6% formaldehyde treatment enhanced the bioavailability of protein in SBM.

Anahtar Kelimeler:

Soybean meal, formaldehyde, by-pass protein, microbial protein synthesis, amino acid absorption

The Effect of Soybean Meal Treated with Formaldehyde on Amount of Protected Protein in the Rumen and Absorption of Amino Acid from Small Intestines

Keywords:

Soybean meal, formaldehyde, by-pass protein, microbial protein synthesis, amino acid absorption,

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Dove, H., Milne, J.A.: Digesta flow and rumen microbial protein production in ewes grazing perennial ryegrass. Aust. J. Agric. Res., 1994; 45: 1229-1245. 2.
Schingoethe, D.J.: Balancing the amino acid needs of dairy cows.
Anim. Feed Sci., Technol., 1996; 60: 153-160.
Mir, Z., Macleod, G.K., Buchanan-Smith, J.G., Grieve, D.G., Grovum, W.L.: Methods for protecting soybean and canola proteins from degradation in the rumen. Can. J. Anim. Sci., 1984; 64: 853-865.
Oldham, J.D.: Protein-energy interrelationships in dairy cows. J. Dairy Sci., 1984; 67: 1090-1114.
Varvikko, T., Lindberg, J.E., Setela, J., Syrjala-Qvist, L.: The effect of formaldehyde treatmant of soyabean meal and rapeseed meal on the amino acids profils and acid-pepsin solubility of rumen undegraded protein. J. Agric. Sci. (Camb.), 1983; 101: 603-612. 6. Deniz, S., Tuncer, fi.D.: Bitkisel protein kaynaklarının formaldehit ile muamele edilmesinin rumende kuru madde ve ham protein ile effektif protein yıkılımı üzerine etkisi. Turk. J. Vet. Anim. Sci., 1995; 19: 1-8.
Yalçın, S., fiehu, A., Karakafl, F.: Ayçiçeği küspesinin formaldehit ve kan ile muamelesinin rumende parçalanma özellikleri ve etkin yıkılabilirliği üzerine etkisi. Turk. J. Vet. Anim. Sci., 1998; 22: 503-509.
Spears, J.W., Clark. J.H., Hatfield. E.E.: Nitrogen utilization and ruminal fermantation in steers fed soybean meal treated with formaldehyde. J. Anim. Sci., 1985; 60: 1072-1080. 9. Fiems, L.O., Cottyn, B.G., Boucqué, Ch.V., Buysse, F.X.: Effect of formaldehyde-treated soybean meal and urea in starters on nitrogen quality, degradability in sacco, sheep digestibility and calf performance. Anim. Feed Sci. Technol., 1987; 16: 287-295.
Atasoy, N., Tafl, A.: Considerations for gastrointestinal cannulation (rumen, duodenum and ileum) in sheep with a ruminal, a simple t-type and a modified t-type cannula. Dtsch. Tierärztl. Wochenschr., 2003; 110: 299-302.
Russell, J.B., Sniffen, C.J., Van Soest, P.J.: Effect of carbohydrate limitation on degradation and utilization of casein by mixed rumen bacteria. J. Dairy Sci., 1983; 66: 763-775.
Association of Official Analytical Chemists Official Methods of Analysis. 15th ed. AOAC, Arlington, VA. 1990.
Van Soest, P.J., Robertson, J.B., Lewis, D.: Methods of dietary fiber, neutral detergent fiber and nonstarch polysaccharides in relation to animal nutrition. J. Dairy Sci., 1991; 74: 3583-3597.
Karslı, M.A.: Ruminal microbial protein synthesis in sheep fed forages of varying nutritive value. PhD Thesis. Iowa State University, Iowa, USA. 1998.
Zinn, R. A., Owens, F.N.: A rapid procedure for purine measurement and its use for estimating net ruminal protein synthesis. Can. J. Anim. Sci., 1986; 66: 157-166.
Williams, C.H., David, D.J., Lismaa, O.I.: The determination of chromic oxide in feces samples by atomic absorption spectrophotometry. J. Agric. Sci., 1962; 59: 381-385.
SAS User’s Guide: Statistics, Version 8.02. SAS Inst. Inc., Cary, NC. 1999.
Steel, R.G., Torrie, J.H.: Principle and Procedures of Statistics (2nd Ed.), McDonald Book Co., Inc., New York, 1980.
Karslı, M.A., Russell, J.R.: Effects of source and concentration of nitrogen and carbohydrate on ruminal microbial protein synthesis. Turk. J. Vet. Anim. Sci., 2002; 26: 201-207.
Lynch, G.L., Berger, L.L., Merchen, N.R., Fahey, G.C. Jr., Baker, E.C.: Effects of heat and alcohol treatments of soybean meal on nitrogen utilization by sheep. J. Anim. Sci., 1987; 65: 235-243.
McKinnon, J.J., Olubobokun, J.A., Christensen, D.A., Cohen, R.D.H.: The influence of heat and chemical treatment on ruminal disappearance of canola meal. Can. J. Anim. Sci., 1991; 71: 773- 780.
Erasmus, L.J., Botha, P.M., Cruywagen, C.W., Meissner, H.H.: Amino acid profile and intestinal digestibility in dairy cows of rumen-undegradable protein from various feedstuffs. J. Dairy Sci., 1994; 77: 541-551.
O’Mara, F.P., Murpy, J.J., Rath, M.: The amino acid composition of protein feedstuffs before and after ruminal incubation and after subsequent passage through the intestines of dairy cows. J. Anim. Sci., 1997; 75: 1941-1949.
Faldet, M.A., Satter, L.D.: Feeding heat-treated full fat soybeans to cows in early lactation. J. Dairy Sci., 1991; 74: 3047-3054.
Tamminga, S.: Protein degradation in the forestomach of ruminants. J. Anim. Sci., 1979; 49: 1615-1630.