Nusret APAYDIN, Zülfikar Kadir SARITAŞ, Kamuran PAMUK, Eser ÖZGENCİL, Fulya OCAK, Tulga ULUS, Baran BUDAK, Selçuk SÜRÜCÜ, Salih Fehmi KATIRCIOĞLU

The Effects on Myocardial Improvement Using ATP-MgCl2 during the Ischemia Reperfusion Period; An In Vitro Study

We investigated the effect of ATP-MgCl2 on myocardial hemodynamics and ultrastructure. In the in vitro study, left ventricular systolic and diastolic pressures and dp/dt values were significantly high in the ATP-MgCl2 group beginning from 0.5 h of the reperfusion period. In the ATP-MgCl2 group, at the end of the third hour, systolic and diastolic pressure values were 80.0 ± 6.2 and 75.2 ± 7.2 mmHg, while in the control group they were 18.4 ± 7.7 and 7.5 ± 6.8 mmHg (P < 0.05). In the ultrastructural analysis, myofibrillary damage was observed in both groups, but the mitochondrial pathologic changes were clearer in the control group. The electron microscopic score was 0.40 ± 0.061 in the ATP-MgCl2 group and 1.2 ± 0.039 in the control group (P < 0.001). The hemodynamic and ultrastructural parameters of the specimens investigated in this study revealed that the usage of ATP-MgCl2 may be beneficial in coping with ischemia-reperfusion damage. In the near future, with the help of more comprehensive studies, ATP-MgCl2 is expected to be used routinely because of its potential benefits.

Anahtar Kelimeler:

In vitro perfusion, ischemia-reperfusion damage, myocardium, ATP-MgCl2

The Effects on Myocardial Improvement Using ATP-MgCl2 during the Ischemia Reperfusion Period; An In Vitro Study

Keywords:

In vitro perfusion, ischemia-reperfusion damage, myocardium, ATP-MgCl2,

PDF

___

Reinhart, R.A.: Clinical correlates of the molecular and cellular actions of magnesium on the cardiovascular system. Am. Heart J., 1991; 121: 1513-1521.
Süzer, Ö.: Langendorff İzole Perfüze Kalp Preperatı ve Kullandığımız Çalıflma Modelleri. In: Bökesoy, T.A. Ed. Türk Farmakoloji Derneği Farmakoloji Eğitiminde Kuflaklararası Bilimsel Etkileflme Seminerleri Programı, Prof. Dr. Reflat Garan Toplantısı. Türk Farmakoloji Derneği Yayınları No: 14, Ankara. 1997; 40-47
Jennings, R.B., Reimer, K.A.: Lethal myocardial ischemic injury. Am. J. Pathol., 1981; 102: 241-255.
Demopoulos, H.B., Flamm, E.S., Pietronigro. D,D., Seligman, M.L.: The free radical pathology and the micro circulation in the major central nervous system disorders. Acta Physiol. Scand. Suppl., 1980; 492: 91-119.
McCord, J.M.: Oxygen derived free radicals in postischemic tissue injury. N. Engl. J. Med., 1985; 312: 159-163.
Headrick, J.P., McKirdy J.C., Willis, R.J.: Functional and metabolic effects of extracellular magnesium in normoxic and ischemic myocardium. Am. J. Physiol., 1998; 275: 917-929.
Rasmussen, H.S., McNair, P., Norregard, P., Backer, V., Lindeneg, O., Balslev, S.: Intravenous magnesium in acute myocardial infarction. Lancet, 1986; 1: 234-236.
Shechter, M., Sharir, M., Labrador, M.J., Forrester, J., Silver, B., Bairey Merz, C.N.: Oral magnesium therapy improves endothelial function in patients with coronary artery disease. Circulation, 2000; 102: 2353-2358.
Pearson, P.J., Evora, P.R., Seccombe, J.F., Schaff, H.V.: Hypomagnesemia inhibits nitric oxide release from coronary endothelium: protective role of magnesium infusiun after cardiac operations. Ann. Thorac. Surg., 1998; 65: 967-972.
Dickens, B.F., Weglicki, W.B., Li, Y.S., Mak, I.T.: Magnesium deficiency in vitro enhances free radical-induced intracellular oxidation and cytotoxicity in endothelial cells. FEBS Lett., 1992; 311: 187-191.
Korthuis, R.J., Grisham, M.B, Zimmerman, B.J., Granger, D.N., Taylor, A.E.: Vascular injury in dogs during ischemia-reperfusion: improvement with ATP-MgCl2pretreatment. Am. J. Physiol., 1998; 254: 702-708.
Kharb, S., Singh, V.: Magnesium deficiency potentiates free radical production asscociated with myocardial infarction. J. Assoc. Physicians India, 2000; 48: 484-485.
Chaudry, I.H., Clemens, M.G., Baue, A.E.: The role of ATP- magnessium in ischemia and shock. Magnesium, 1986; 5: 211- 220
Chaudry, I.H., Stephan, R.N., Dean, R.E., Clemens, M.G., Baue, A.E.: Use of magnesium-ATP following liver ischemia. Magnesium, 1988; 7: 68-77
Simpson, J.I., Eide, T.R., Schiff, G.A., Clagnaz, J.F., Hossain, I., Tverskoy, A., Koski, G.: Intratechal magnesium sulfate protects the spinal cord from ischemic injury during thoracic aortic cross- clamping. Anesthesiology, 1994; 81: 1493-1499.
Katırcıoğlu, S.F., Ulus, A.T., Sarıtafl, Z., Gökçe, P.: Effects of ATP- MgCl2administration in hypovolemic dogs. Panminerva Med., 1999; 41: 323-330.
Ulus, A.T., Sarıtafl, Z., Yamak, B., Sürücü, S., Tuncer, M., Katırcıoğlu, S.F.: ATP-MgCl2utilization for spinal cord protection during experimental thoracic aortic occlusion. J. Cardiovasc. Surg., 1999; 40: 495-499.