2010

Levels of some ovarian hormones in the Pre-and post spawning periods of Chalcalburnus tarichi Pallas, 1811, and the postovulatory structure of follicles

Bu çalışmada, inci kefalinde yumurta bıraktıktan sonra foliküllerin yapısı ve yumurta bırakmadan önce ve yumurta bıraktıktan sonra ovaryum 11 -dehydrocorticosteroit (11-DHC), östradiol-17(3 (E2), 17oc-hydroxyprogesteron (17a-0H-P) ve progesteron (P) seviyeleri araştırıldı. Ovulasyondan sonra foliküllerin bol damarlı bir teka tabakası ve bol miktarda düz endoplazmik retikulum, tubüler kristali çok sayıda mitokondri ve lipit damlaları içeren büyük granüioza hücreleri ile karakterize edildiği gösterilmiştir. Ovaryum 11-DHC seviyesi yumurta bırakmadan önce ve sonra düşüktür (38,3 ± 4,9-22,3 ± 2,6 ng/ml). E2 seviyesi yumurta bırakmadan önce 216,8 ± 31,1 ng/ml olarak belirlendi. Bu hormon yumurta bıraktıktan sonra 1. ve 5. günde azaldı ve 10. günde tekrar 216,8 ± 6,7 ng/ml'ye yükseldi. Bununla birlikte, E2 seviyesi 15. günde belirgin olarak düştü (61,5 ± 17,8 ng/ml) (P < 0,01). 17oc-OH-P seviyesi yumurta bırakmadan önce düşüktü (84,5 ± 49,4 ng/ml) ve yumurta bıraktıktan sonra artarak 10. günde en yüksek seviyeye ulaştı (295,7 ± 52,5 ng/ml) fakat 15. günde hafif bir düşüş gözlendi (190 ± 21,5 ng/ml) (P > 0,01). P seviyesi yumurta bırakmadan önce düşüktü (23,4 ± 18,3 ng/ml). Onun seviyesi yumurta bıraktıktan sonra artmaya başladı ve 10. günde maksimum seviyeye ulaştı (200,7 ± 29,1 ng/ml) (P < 0,01) fakat 15. günde belirgin olarak düştü (63,2 ± 19,5 ng/ml) (P < 0,01). Bu çalışmadan elde edilen sonuçlar: a) Ovulasyondan sonraki foliküllerde granüioza hücrelerinin steroitsentezlediğini, b) 11-DHC'nin yumurta bırakmadan önce ve sonra etkili olmadığını, c) E2'nin yumurta bırakmayı uyardığını, d) 17a-0H-P ve P'un yumurta bıraktıktan sonra etkili olduğunu gösterir. İnci kefalinde, ovulasyondan sonraki foliküllerde apoptotik hücrelerin, E2 and P'nin yumurta bıraktıktan sonra en düşük seviyede olduğu, 15. günde arttığı sonucu çıkarılabilir.

İnci kefali' nin (Chalcalburnus tarichi Pallas, 1811) yumurta bırakmadan önce ve sonraki periyotta bazı ovaryum hormon seviyeleri ve foliküllerin ovulasyondan sonraki yapısı

The structure of postovulatory follicles and the levels of ovarian 11 -dehydrocorticosterone (11 -DHC), estradiol-17(3 (E2), 17oc-hydroxyprogesterone(17a-OH-P), and progesterone (P), before and after spawning, were studied in Chalcalburnus tarichi. It was observed that postovulatory follicles are characterized by a highly vascular thecal layer and hypertrophied granulosa cells containing dense smooth endoplasmic reticulum, many large mitochondria with tubular cristae, and lipid droplets. Ovary 11-DHC level was low in the pre- and post-spawning periods (38.3 ± 4.9-22.3 ± 2.6 ng/ml). The level of E2 was 216.8 ±31.1 ng/ml before spawning. This hormone declined on the 1st and 5th day after spawning and increased again to the level of 216.8 ± 6.7 ng/ml on the 10th day; however, the E2 level decreased significantly on the 15th day (61.5 ± 17.8 ng/ml) (P < 0.01). The 17oc-OH-P level was low (84.5 ± 49.4 ng/ml) before spawning and reached a peak (295.7 ± 52.5 ng/ml) on the 10th postspawning day, but a slight decline was observed on the 15th day (190 ± 21.5 ng/ml) (P > 0.01). The P level was low (23.4 ± 18.3 ng/ml) before spawning. Its level began to increase after spawning and reached a peak on the 10th postspawning day (200.7 ± 29.1 ng/ml) (P < 0.01), but declined significantly on the 15th day (63.2 ± 19.5 ng/ml) (P < 0.01). The results obtained from this study indicate that: a) In the postovulatory follicles, the granulosa cells produce steroids; b) 11 -DHC has no effect before or after spawning; c) E2 induces spawning; d) 17a-0H-P and P have an effect after spawning, it can be concluded that the apoptotic cells in postovulatory follicles increased on the 15th day, at which time E2 and P hormones were at their lowest levels after spawning in C. tarichi.

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