The effect of flashed light on the ultrastructure of the retinal photoreceptor cells in rabbit: a transmission electron microscope study

The effects of light exposure on the retina have been carefully considered in recent years. The purpose of the current study was to investigate the effect of flashed light with low and high intensity on the photoreceptor layer of rabbit. Fifteen adult male New Zealand White rabbits were randomly divided into 3 groups: a control group, experimental group I (exposed to 60 W of flashed light 5 times), and experimental group II (exposed to 1000 W of flashed light 5 times). The retinas of the eyes were removed and studied by transmission electron microscope. The photoreceptor layer damage in experimental group II contained outer segment loss, vacuolated and disorganized mitochondria, vacuolization, cell swelling, and pyknotic, karyorrhexis, and karyolytic nuclei. The results suggested that high-intensity flashed light (1000 W) caused more photoreceptor layer damage than low-intensity light (60 W).

The effect of flashed light on the ultrastructure of the retinal photoreceptor cells in rabbit: a transmission electron microscope study

The effects of light exposure on the retina have been carefully considered in recent years. The purpose of the current study was to investigate the effect of flashed light with low and high intensity on the photoreceptor layer of rabbit. Fifteen adult male New Zealand White rabbits were randomly divided into 3 groups: a control group, experimental group I (exposed to 60 W of flashed light 5 times), and experimental group II (exposed to 1000 W of flashed light 5 times). The retinas of the eyes were removed and studied by transmission electron microscope. The photoreceptor layer damage in experimental group II contained outer segment loss, vacuolated and disorganized mitochondria, vacuolization, cell swelling, and pyknotic, karyorrhexis, and karyolytic nuclei. The results suggested that high-intensity flashed light (1000 W) caused more photoreceptor layer damage than low-intensity light (60 W).

___

  • Esfandiari, A., Yousofi, A.R., Dehghan, A., Safavi, A.: Effect of intermittent light on the photoreceptor cells of the retina in rabbits. Jpn. J. Ophthalmol., 2009; 53: 635–639.
  • Organisciak, D.T., Jiang, Y.L., Wang, H.M., Pickford, M., Blanks, J.C.: Retinal light damage in rats exposed to intermittent light. Comparison with continuous light exposure. Invest. Ophthalmol. Vis. Sci., 1989; 30: 795–805.
  • Hansson, H.A.: A histochemical study of cellular reactions in rat retina transiently damaged by visible light. Exp. Eye. Res., 1971; 12: 270–274.
  • Noell, W.K.: Possible mechanisms of photoreceptor damage by light in mammalian eyes. Vision Res., 1980; 20: 1163–1171.
  • Williams, T.P., Howell, W.L.: Action spectrum of retinal light damage in albino rats. Invest. Ophthalmol. Vis. Sci., 1983; 24: 285–287.
  • La Vail, M.M., Gorrin, G.M., Repaci, M.A., Thomas, L.A., Ginsberg, H.M.: Genetic regulation of light damage to photoreceptors. Invest. Ophthalmol. Vis. Sci., 1987; 28: 1043 –10
  • White, M.P., Fisher, L.J.: Degree of light damage to the retina varies with time of day of bright light exposure. Physiol. Behav., 1987; 39: 607–613.
  • Braekevelt, C.R.: Photoreceptor fine structure in light- and dark-adaptation in the butterfly fish (Pantodon buchholzi). Anat. Anz., 1990; 171: 351–358.
  • Braekevelt, C.R., Smith, S.A., Smith, B.J.: Photoreceptor fine structure in oreochromis niloticus in light- and darkadaptation. Anat. Rec., 1998; 252: 453–461.
  • Noell, W.K., Albrecht, R.: Irreversible effects of visual light on the retina, role of vitamin A. Science, 1971; 172: 76–79.
  • Lanum, J.: The damaging effects of light on the retina. Empirical findings, theoretical and practical implications. Surv. Ophthalmol., 1978; 22: 221–249.
  • Penn, J.S., Anderson, R.E.: Effect of light history on the rat retina. In: Osborne, N., Chader, G.J., Eds. Progress in Retinal Research. Pergamon Press, Oxford. 1990; 75–98.
  • Organisciak, D.T., Wang, H.M., Noell, W.K.: Aspects of the ascorbate protective mechanism in retinal light damage of rats with normal and reduced ROS docosahexaenoic acid. Prog. Clin. Biol. Res., 1987; 247: 455–468.
  • Koutz, C.A., Wiegand, R.D., Rapp, L.M., Anderson, R.E.: Effect of dietary fat on the response of the rat retina to chronic and acute light stress. Exp. Eye Res., 1995; 60: 307–316.
  • Borges, J.M., Edward, D.P., Tso, M.O.: A comparative study of photic injury in four inbred strains of albino rats. Curr. Eye Res., 1990; 9: 799–803.
  • Osteen, W.K., Anderson, K.V., Shear, C.R.: Photoreceptor degeneration in albino rats: dependency on age. Invest. Ophthalmol., 1974; 13: 334–339.
  • De Lint, PJ., Van Norren, D., Toebosch, A.M.: Effect of body temperature on threshold for retinal light damage. Invest. Ophthalmol. Vis. Sci. 1992; 33: 2382–2387.
  • Organisciak, D.T., Darrow, R.M., Noell, W.K., Blanks, J.C.: Hyperthermia accelerates retinal light damage in rats. Invest. Ophthalmol. Vis. Sci., 1995; 36: 997–1008.
  • Olfert, E.D., Cross, B.M., McWilliam, A.A.: Guide to the Care and Use of Experimental Animals, Canadian Council on Animal Care, 2nd ed., Vol. 1. 1993. Available at: http://www. ccac.ca/Documents/Standards/Guidelines/Experimental_ Animals_Vol1.pdf. Accessed 8 September 2011.
  • Young, R.W., Bok, D.: Participation of the retinal pigment epithelium in the rod outer segment renewal process. J. Cell Biol., 1969; 42: 392–403.
  • La Vali, M.M:. Rod outer segment disc shedding in rat retina: relationship to cyclic lighting. Science, 1976; 194: 1071–1074.
  • Watson, C.L., Gold, M.R.: Lysophosphatidylcholine modulates cardiac I (Na) via multiple protein kinase pathways. Circ. Res., 1997; 81: 387–395.
Turkish Journal of Veterinary and Animal Sciences-Cover
  • ISSN: 1300-0128
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK
Sayıdaki Diğer Makaleler

Lipid peroxidation in neoplastic tissue of dogs with mammary cancer fed with different kinds of diet

Maria KARAYANNOPOULOU, Anna FYTIANOU, Nikolaos ASSALOUMIDIS

Fungal peritonitis in dog caused by Candida albicans - a case report and literature overview

Kamila GLINSKA, Marcin JANKOWSKI, Krzysztof KUBIAK, Jolanta SPUZAK, Maciej GRZEGORY, Stanislaw DZIMIRA

Evaluation of the pathogenicity of Candida zeylanoides in BALB/c mice

Ali Reza KHOSRAVI, Hojjatollah SHOKRI, Donya NIKAEIN

The effect of electron beam irradiation on β-glucan content, X-ray diffraction of starch, protein subunit patterns, and in vivo digestibility of barley grain in cockerels

Parvin SHAWRANG, Ali Asghar SADEGHI, Behnam GHORBANI

The effect of electron beam irradiation on β-glucan content, X-ray diffraction of starch, protein subunit patterns, and in vivo digestibility of barley grain in cockerels

Parvin SHAWRANG, Ali Asghar SADEGHI, Behnam GHORBANI

A survey study on hantavirus, cowpox virus, and Leptospira infections in Microtus hartingi in Kırşehir Province, Central Anatolia, Turkey

Ahmet Kürşat AZKUR, Sedat KAYGUSUZ, Muhammet Eren ASLAN

Vertical transmission of porcine circovirus 2b (PCV2b) to mouse fetuses in maternal uterus by artificial insemination with semen from PCV2b-infected male mouse

Deng ZHIBANG, Gong QIANLONG, Wang NAIDONG, Yuan ANWEN, Yu Xing LONG

Effect of corpus luteum: quality and recovery rate of buffalo (Bubalus bubalis) oocytes

Lakshman SAHOO, Suresh K. SINGLA

Immunokinetics of Theileria equi specific antibodies: a comparison in serial and single dilution ELISA antibody end titres

Yogesh KUMAR, Dharam Vir MALHOTRA, Anil Kumar NICHANI, Ashok KUMAR, Shruti DHAR, Sanjay KUMAR

Ultrasonographic evaluation of buffalo eyes

Gholamreza ASSADNASSAB, Majid FARTASHVAND