The comparing of homogenization methods for oxidative and antioxidative analyses of sperm
The comparing of homogenization methods for oxidative and antioxidative analyses of sperm
Pretreatment of sperm for oxidative and antioxidative analysis has been shown to be different in studies. These differencescan affect the result of analyses. The aim of this study was to compare the analyses of sperm homogenization methods. The semenof four rams were used in the study. Pretreatment procedures included the rotary, bead, and ultrasonic homogenization methods.Oxidative and antioxidative status of sperm samples was assessed by measuring serum lipid hydroperoxide, total oxidant status, freesulfhydryl groups (SH total thiol), ceruloplasmin, and total antioxidant capacity levels. As a result, the homogenization methods beforeoxidative and antioxidative status analyses significantly affected the result (P < 0.05), and the ultrasonic homogenization method wasthe most effective. It is recommended to use the ultrasonic homogenization as a pretreatment method to determine the oxidative andantioxidative status analyses in sperm.
___
- 1. Theodorsson E. Validation and verification of measurement
methods in clinical chemistry. Bioanalysis 2012; 4: 305-320.
- 2. Nadjarzadeh A, Shidfar F, Amirjannati N, Vafa MR,
Motevalian SA, Gohari MR, Sadeghi MR. Effect of Coenzyme
Q10 supplementation on antioxidant enzymes activity and
oxidative stress of seminal plasma: a double-blind randomised
clinical trial. Andrologia 2014; 46 (2): 177-183.
- 3. Bucak MN, Atessahin A, Varisli O, Yuce A, Tekin N, Akcay A.
The influence of trehalose, taurine, cysteamine and hyaluronan
on ram semen microscopic and oxidative stress parameters
after freeze-thawing process. Theriogenology 2007; 67 (5):
1060-1067.
- 4. Yigitbasi T, Baskin Y, Celik H, Karaarslan F, Taheri C, Afacan
G, Erel O. The evaluation of total antioxidant and oxidant
response in seminal plasma in fertile and infertile men using a
novel automated method. Turkiye Klinikleri J Med Sci 2011; 31
(1): 61-67.
- 5. Tuncer PB, Sariozkan S, Bucak MN, Ulutas PA, Akalin PP,
Buyukleblebici S, Canturk F. Effect of glutamine and sugars
after bull spermatozoa cryopreservation. Theriogenology 2011;
75 (8): 1459-1465.
- 6. Orzolek A, Wysocki P, Strzeżek J, Kordan W. Superoxide
dismutase (SOD) in boar spermatozoa: purification,
biochemical properties and changes in activity during semen
storage (16 °C) in different extenders. Reprod Biol 2013; 13 (1):
34-40.
- 7. Bucak MN, Tuncer PB, Sarıözkan S, Başpınar N, Taşpınar M,
Coyan K, Bilgili A, Akalın PP, Büyükleblebici S, Aydos S et
al.. Effects of antioxidants on post-thawed bovine sperm and
oxidative stress parameters: antioxidants protect DNA integrity
against cryodamage. Cryobiology 2010; 61 (3): 248-253.
- 8. Venkatesh S, Shamsi MB, Dudeja S, Kumar R, Dada R. Reactive
oxygen species measurement in neat and washed semen:
comparative analysis and its significance in male infertility
assessment. Arch Gynecol Obstet 2011; 283 (1): 121-126.
- 9. de Lamirande E, Gagnon C. Reactive oxygen species and
human spermatozoa. I. Effects on the motility of intact
spermatozoa and on sperm axonemes. J Androl 1992; 13 (5):
368-378.
- 10. Siciliano L, Tarantino P, Longobardi F, Rago V, De Stefano C,
Carpino A. Impaired seminal antioxidant capacity in human
semen with hyperviscosity or oligoasthenozoospermia. J
Androl 2001; 22 (5): 798-803.
- 11. Said TM, Agarwal A, Sharma RK, Mascha E, Sikka SC, Thomas
AJ. Human sperm superoxide anion generation and correlation
with semen quality in patients with male infertility. Fertility
and Sterility 2004; 82: 871-877.
- 12. Rhemrev JPT, Van Overveld F, Haenen G, Teerlink T, Bast A
and Vermeiden JPW. Quantification of the nonenzymatic past
and slow TRAP in a postaddition assay in human seminal
plasma and the antioxidant contributions of various seminal
compounds. J Androl 2000; 21 (6): 913-920.
- 13. Ford WCL. Regulation of sperm function by reactive oxygen
species. Hum Reprod Update 2004; 10 (5): 387-399.
- 14. Surai PF, Fujihara N, Speake BK, Brillard JP, Wishart GJ,
Sparks NHC. Polyunsaturated fatty acids, lipid peroxidation
and antioxidant protection in avian semen. Asian Australas J
of Anim Sci 2001; 14 (7): 1024-1050.
- 15. Georgieva NV. Oxidative stress as a factor of disrupted
ecological oxidative balance in biological systems a review.
Bulgarian Journal of Veterinary Medicine. 2005; 8 (1): 1.
- 16. Menditto A, Pietraforte D, Minetti M. Ascorbic acid in human
seminal plasma is protected from iron-mediated oxidation,
but is potentially exposed to copper-induced damage. Human
Reproduction 1997; 12: 1699-1705.
- 17. Musabekov A, Rustenov A, Shapiev I, Eleugalieva N. Use of
thiol compounds for preservation of functional cryopreserved
sperm of boar. In: Proceedings of the 2nd International
Conference on Agricultural and Animal Science. Singapore:
IPCBEE; 2011. pp. 170-174.
- 18. Aydilek N, Varışlı Ö, Selek Ş, Korkmaz Ö, Atlı MO, Taşkın
A. The effect of estrous cycle on oxidant and antioxidant
parameters in dairy cows. Kafkas Univ Vet Fak Derg 2014; 20
(5): 703-709.
- 19. Sariozkan S, Tuncer PB, Buyukleblebici S, Bucak MN, Canturk
F, Eken A. Antioxidative effects of cysteamine, hyaluronan and
fetuin on post-thaw semen quality, DNA integrity and oxidative
stress parameters in the Brown Swiss bull. Andrologia 2015; 47
(2): 138-147.
- 20. Naijian HR, Kohram H, Shahneh AZ, Sharafi M, Bucak MN.
Effects of different concentrations of BHT on microscopic and
oxidative parameters of Mahabadi goat semen following the
freeze-thaw process. Cryobiology 2013; 66 (2): 151-155.
- 21. Gavella M, Lipovac V, Vucic M, Rocic B. Superoxide anion
scavenging capacity of human seminal plasma. Int J Androl
1996; 19 (2): 82-90.
- 22. Verit FF, Verit A, Ciftci H, Erel O, Celik H. Paraoxonase-1
activity in subfertile men and relationship to sperm parameters.
J Androl 2009; 30: 183-189.
- 23. Varışlı Ö, Taşkın A, Akyol N. Effects of different extenders
and additives on liquid storage of Awassi ram semen. Turkish
Journal of Veterinary and Animal Sciences. 2018; 42 (4): 230-
242.
- 24. Erel O. Automated measurement of serum ferroxidase activity.
Clinical chemistry 1998; 44 (11): 2313-2319.
- 25. Swain JA, Darley-Usmar V, Gutteridge JM. Peroxynitrite
releases copper from caeruloplasmin: implications for
atherosclerosis. FEBS Lett 1994; 342: 49-52.
- 26. Winkles J, Jones AF, Winyard P, Blake DR, Lunec J. An
automated method for the kinetic measurement of ferroxidase
activity. Ann Clin Biochem 1988; 25: 250-254.