A diagnostic survey of Neospora caninum infection in aborted fetuses in the Middle Black Sea Region and Sivas Province, Turkey
A diagnostic survey of Neospora caninum infection in aborted fetuses in the Middle Black Sea Region and Sivas Province, Turkey
Neospora caninum is a major, cosmopolitan, protozoan parasite associated with abortion in cattle and water buffaloes. In thisstudy, real-time PCR was used for the detection and quantification of the burden of N. caninum infections in aborted fetuses in theMiddle Black Sea Region and Sivas Province of Turkey. In total, N. caninum DNA was detected in 44 of the 89 (49.4%) aborted fetusesof the cattle and water buffalo from 89 different farms. The authors conclude that the pervasiveness of neosporosis is a threat to cattlebreeding in the Middle Black Sea Region and Sivas Province. Real-time PCR testing gave consistently reliable results, which suggeststhat this procedure can be used for the molecular diagnosis of abortion cases in bovine livestock.
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- 1. Dubey JP, Schares G. Diagnosis of bovine neosporosis. Veterinary
Parasitology 2006; 140: 1-34. doi: 10.1016/j.vetpar.2006.03.035
- 2. Reichel MP, Ayanegui-Alcerreca MA, Gondim LFP, Ellis JT.
What is the global economic impact of Neospora caninum in
cattle-the billion dollar question. International Journal for
Parasitology 2013; 43: 133-142. doi: 10.1016/j.ijpara.2012.10.022
- 3. Dubey JP, Schares G, Ortega-Mora LM. Epidemiology and
control of neosporosis and Neospora caninum. Clinical
Microbiology Reviews 2007; 20: 323-367. doi: 10.1128/
CMR.00031-06
- 4. Dubey JP, Schares G. Neosporosis in animals - the last five
years. Veterinary Parasitology 2011; 180: 90-108. doi: 10.1016/j.
vetpar.2011.05.031
- 5. Dubey JP, Carpenter JL, Speer CA, Topper MJ, Uggla A. Newly
recognized fatal protozoan disease of dogs. Journal of the
American Veterinary Medical Association 1988; 192: 1269-
1285.
- 6. Sawada M, Kondo H, Tomioka Y, Park C, Morita T et al.
Isolation of Neospora caninum from the brain of a naturally
infected adult dairy cow. Veterinary Parasitology 2000; 90: 247-
252. doi: 10.1016/S0304-4017(00)00248-X
- 7. Auriemma C, Lucibelli MG, Borriello G, De Carlo E,
Martucciello A et al. PCR detection of Neospora caninum in
water buffalo fetal tissues. Acta Parasitologica 2014; 59: 1-4.
doi: 10.2478/s11686-014-0201-y
- 8. Akça A, Gökçe HI, Guy CS, Mc Garry JW, Williams DJ.
Prevalence of antibodies to Neospora caninum in local and
imported cattle breds in the Kars Province of Turkey. Research
in Veterinary Science 2005; 78: 123-126. doi: 10.1016/j.
rvsc.2004.08.006
- 9. Aktaş M, Şaki CE, Altay K, Şimşek S, Ütük AE et al. Survey of
Neospora caninum in cattle in some provinces in the Eastern
Anatolian Region. Turkiye Parazitoloji Dergisi 2005; 29: 22-25.
- 10. Albayrak H, Ozan E, Beyhan YE, Kurt M, Kılıçoğlu Y. A
serological investigation of some aetiological agents associated
with abortion in domestic water buffalo (Bubalus bubalis
Linneaus, 1758) in Samsun province of northern Turkey.
Atatürk Üniversitesi Veteriner Bilimleri Dergisi 2012; 7: 155-
160.
- 11. Öcal N, Atmaca HT, Albay MK, Deniz A, Kalender H et al. A
new approach to Neospora caninum infection epidemiology:
neosporosis in integrated and rural dairy farms in Turkey.
Turkish Journal of Veterinary and Animal Sciences 2014; 38:
161-168. doi: 10.3906/vet-1307-11
- 12. Calandra PM, Di Matia JM, Cano DB, Odriozola EA, García
JA et al. Neosporosis epidemica y endemica: descripcion
de dos eventos en bovinos para cria. Revista Argentina de
Microbiología 2014; 46: 315-319 (In Spanish). doi: 10.1016/
S0325- 541(14)70089-6
- 13. Thurmond MC, Hietala SK. Effect of congenitally acquired
Neospora caninum infection on risk of abortion and subsequent
abortions in dairy cattle. American Journal of Veterinary
Research 1997; 58(12):1381-1385.
- 14. Hernandez J, Risco C, Donovan A. Risk of abortion associated
with Neospora caninum during different lactations and
evidence of congenital transmission in dairy cows. Journal of
the American Veterinary Medical Association 2002; 221: 1742-
1746.
- 15. Duivenvoorden J, Lusis P. Neospora abortions in eastern Ontario
dairy herds. The Canadian Veterinary Journal 1995; 36(10): 623.
- 16. Kul O, Kabakçı N, Yıldız K, Öcal N, Kalender H et al. Neospora
caninum associated with epidemic abortions in dairy cattle:
The first clinical neosporosis report in Turkey. Veterinary
Parasitology 2009; 159: 69-72. doi: 10.1016/j.vetpar.2008.10.019
- 17. Özkaraca M, İrehan B, Parmaksız A, Ekinci Aİ, Çomaklı S.
Determination of Neospora caninum and Toxoplasma gondii in
aborted bovine foetuses by duplex PCR, immunohistochemistry
and immunofluorescence methods. Medycyna Weterynaryjna
2017; 73: 346-351. doi: 10.21521/mw.5707
- 18. Pişkin FÇ, Ütük AE. Prevalence of Neospora caninum in cows
with stillbirth and abortion. Etlik Veteriner Mikrobiyoloji
Dergisi 2009; 20: 23-26.
- 19. Erol U, Danyer E, Tuncer S, Korkmaz Ç, Deniz A. Atık yapan
sığırlarda anti-Neospora caninum antikorlarının yaygınlığının
araştırılması. Etlik Veteriner Mikrobiyoloji Dergisi 2019; 30 (1):
78-81 (In Turkish).
- 20. Mor N, Akça A. Kars yöresinde sığır ve köpeklerde Neospora
caninum üzerine epidemiyolojik araştırmalar: Gruplararası
çalışma. Kafkas Universitesi Veteriner Fakültesi Dergisi
2012; 18 (Suppl-A): A193-A199 (In Turkish). doi: 10.9775/
kvfd.2012.6181
- 21. Karatepe B, Karatepe M. Seroprevalence of Neospora caninum
in cattle in Niğde Province, Turkey. Israel Journal of Veterinary
Medicine 2016; 71: 39-42.
- 22. Sevgili M, Altaş MG, Keskin O. Seroprevalence of Neospora
caninum in cattle in the province of Şanlıurfa. Turkish Journal
of Veterinary and Animal Sciences 2005; 29: 127-130.
- 23. Vural G, Aksoy E, Bozkır M, Küçükayan U, Ertürk A.
Seroprevalence of Neospora caninum in dairy cattle herds in
Central Anatolia, Turkey. Veterinarski Arhiv 2006; 76: 343-
349.
- 24. Dubey JP. Review of Neospora caninum and neosporosis in
animals. The Korean Journal of Parasitology 2003; 41: 1-16.
doi: 10.3347/kjp.2003.41.1.1
- 25. Chryssafidis AL, Canton G, Chianini F, Innes EA, Madureira
EH et al. Abortion and fetal lesions induced by Neospora
caninum in experimentally infected water buffaloes (Bubalus
bubalis). Parasitology Research 2015; 114: 193-199. doi:
10.1007/s00436-014-4178-0
- 26. Reichel MP, McAllister MM, Nasir A, Moore DP. A review
of Neospora caninum in water buffalo (Bubalus bubalis).
Veterinary Parasitology 2015; 212: 75-79. doi: 10.1016/j.
vetpar.2015.08.008
- 27. Pereira-Bueno J, Quintanilla-Gozalo A, Pérez-Pérez V, Espi-
Felgueroso A, Alvarez-García G et al. Evaluation by different
diagnostic techniques of bovine abortion associated with
Neospora caninum in Spain. Veterinary Parasitology 2003; 111:
143-152.
- 28. Medina L, Cruz-Vazquez C, Quezada T, Morales E, García-
Vázquez Z. Survey of Neospora caninum infection by nested
PCR in aborted fetuses from dairy farms in Aguascalientes,
Mexico. Veterinary Parasitology 2006; 136: 187-191. doi:
10.1016/j.vetpar.2005.11.003
- 29. Yıldız K, Kul O, Babür C, Kılıç S, Gazyağcı AN et al.
Seroprevalence of Neospora caninum in dairy cattle ranches
with high abortion rate: Special emphasis to serologic co -
existence with Toxoplasma gondii, Brucella abortus and Listeria
monocytogenes. Veterinary Parasitology 2009; 164: 306-310.
doi: 10.1016/j.vetpar.2009.06.004
- 30. Kaçar C, Gökçe Hİ, Akça A, Güngör Ö, Kaya S. Seroprevalence
of Neospora caninum in cows with abortion history and in dogs
sharing the same area in the Kars Region, Turkey. Revue de
Medecine Veterinaire 2012; 163: 343-347.
- 31. Çelik HA, Kozan E, Eser M, Yılmaz O, Birdane MK et al. A
research on seroprevalence of Neospora caninum in cattle.
Ankara Universitesi Veteriner Fakültesi Dergisi 2013; 60: 99-
102.
- 32. Collantes-Fernandez E, Zaballos A, Alvarez-Garcia G, Ortega-
Mora LM. Quantitative detection of Neospora caninum in
bovine aborted fetuses and experimentally infected mice by
Real-time PCR. Journal of Clinical Microbiology 2002; 40:
1194-1198. doi: 10.1128/JCM.40.4.1194–1198.2002
- 33. Cabral AD, Camargo CN, Galleti NTC, Okuda LH, Pituco
EM et al. Diagnosis of Neospora caninum in bovine fetuses by
histology, immunohistochemistry, and nested-PCR. Revista
Brasileira de Parasitologia Veterinária 2009; 18: 14-19. doi:
10.4322/rbpv.01804003
- 34. Yao L, Yang N, Liu Q, Wang M, Zhang W et al. Detection of
Neospora caninum in aborted bovine fetuses and dam blood
samples by nested PCR and ELISA and seroprevalence in
Beijing and Tianjin, China. Parasitology 2009; 136: 1251-1256.
doi: 10.1017/S0031182009990813