Effect of exercise on some minerals, metabolites and enzyme activities in the serum of trained Arabian horses
Effect of exercise on some minerals, metabolites and enzyme activities in the serum of trained Arabian horses
The exercise-induced variations of some biochemical parameters including calcium (Ca), phosphorous (P), iron (Fe),copper (Cu), manganese (Mn), selenium (Se), zinc (Zn), sodium (Na), potassium (K), chlorine (Cl), glucose, urea, creatinine, lactatedehydrogenase (LDH), aspartate aminotransferase (AST), alanine aminotransferase (ALT), and creatine kinase (CK) were assessed inthe serum of 25 Arabian horses. Blood samples were taken from the jugular vein 3 different times: before exercise, 5 h after exercise, and18 h after exercise. Statistical analysis was performed using SAS (version 9.2; SAS Institute, Cary, NC, USA). Ca, Cl, glucose, and ureashowed significant (P < 0.05) changes due to exercise. There was significant increase from before exercise to 5 h after exercise in ureaconcentrations while Ca and Cl concentrations significantly decreased. A significant ascending trend for Ca between 5 h and 18 h afterexercise was also seen, while glucose and urea had a significantly descending trend. No significant time-based variations were observedfor other variables. The results of the present study indicated that exercise could affect the value of some biochemical parameters, whichcould be used in future studies evaluating the health status of Arabian horses.
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- 1. Adamu L, Adzahan NM, Rasedee A, Ahmad B. Responses of
serum biochemical parameters, electrolytes and heart rate in
an 80km endurance race. Journal of Veterinary Advances 2014;
4: 329-337.
- 2. Hassan HY, Aly MA, Elseady YM, Nayel MA, Elsify AM et al.
The effect of race in the clinical, hematological and biochemical
biomarkers in Thoroughbred horses. Alexandria Journal of
Veterinary Science 2015; 46: 161-169.
- 3. Cocq P, Munsters C. Performance monitoring in equine
sports. In: Proceedings of the 7th International Conference on
Methods and Techniques in Behavioral Research; Eindhoven,
the Netherlands; 2018. pp. 13-15.
- 4. Yur F, Dede S, Deger Y, Kilicalp D. Effects of vitamin E and
selenium on serum trace and major elements in horses.
Biological Trace Element Research 2008; 125: 223-228.
- 5. Şovărel E. Research regarding blood glucose dynamic in
horses used at complete trial competition. Agriculture and
Agricultural Science Procedia 2016; 10: 323-327.
- 6. Fazio F, Casella S, Assenza A, Arfuso F, Tosto F et al. Blood
biochemical changes in show jumpers during a simulated show
jumping test. Veterinarski Arhiv 2014; 84: 143-152.
- 7. Larsson J, Pilborg PH, Johansen M, Christophersen MT, Holte
A et al. Physiological parameters of endurance horses precompared
to post-race, correlated with performance: A two
race study from Scandinavia. International Scholary Research
Notices: Veterinary Science 2013; 2013: 684353.
- 8. Assenza A, Bergero D, Congiu F, Tosto F, Giannetto C
et al. Evaluation of serum electrolytes and blood lactate
concentration during repeated maximal exercise in horse.
Journal of Equine Veterinary Science 2014; 34: 1175-1180.
- 9. Escalera F, Gonzalez J, Alonso ME, Lomillos JM, Gaudioso
VR. Influence of intense exercise on acid-base, blood gas and
electrolyte status in bulls. Research in Veterinary Science 2013;
95: 623-628.
- 10. Adamu L, Noraniza MA, Rasedee A, Bashir A. Effect of age
and performance on physical, hematological, and biochemical
parameters in endurance horses. Journal of Equine Veterinary
Science 2013; 33: 415-420.
- 11. Aguilera-Tejero E, Estepa JC, Lopez I, Bas S, Garfia B et al.
Plasma ionized Ca and parathyroid hormone concentrations
in horses after endurance rides. Journal of the American
Veterinary Medical Association 2001; 219: 488-490.
- 12. Padilha FGF, Dimache LAG, Almeida FQD, Ferreira AMR.
Blood biochemical parameters of Brazilian sport horses under
training in tropical climate. Revista Brasileira de Zootecnia
2017; 46: 678-682.
- 13. Teixeira-Neto AR, Ferraz GC, Moscardini ARC, Balsamao
GM, Souza JCF et al. Alterations in muscular enzymes of
horses competing long-distance endurance rides under
tropical climate. Arquivo Brasileiro de Medicina Veterinária e
Zootecnia 2008; 60: 543-549.
- 14. Backhouse JW. The implications of heat generation and
electrolytes in feed and competition in athletic horses. Asian-
Australian Journal of Animal Science 2000; 13: 394-399.
- 15. Soroko M, Bajerska KS, Zaborski D, Pozniak B, Dudek K et
al. Exercise-induced changes in skin temperature and blood
parameters in horses. Archives Animal Breeding 2019; 62: 205-
213
- 16. Martins LP, Di-Filippo PA, Meireles MAD, Pecanha RMS, Mello
LDM et al. Effect of marcha exercise on serum electrolytes and
acid-base balance in Mangalarga Marchador horses. Journal of
Equine Veterinary Science 2017; 49: 108-112.
- 17. Vervuert I, Coenen M, Wedemeyer U, Chrobok C. Calcium
homeostasis and intact plasma parathyroid hormone during
exercise and training in young Standardbred horses. Equine
Veterinary Journal 2002; 34: 713-718.
- 18. Allaam M, Elseady Y, Nayel M, Elsify A, Salama A et
al. Physiological and hemato-chemical evaluation of
thoroughbred race horse after exercise. International Journal
of Agro Veterinary and Medical Science 2014; 8: 81-93.
- 19. Grimston SK, Tanquay KE, Gundberg CM, Hanley DA. The
calciotropic hormone response to changes in serum Ca during
exercise in mare long distance runners. Journal of Clinical
Endocrinology & Metabolism 1993; 76: 867-872.
- 20. Alosia JF, Rasulo P, Deftos LJ, Vaswani A, Yeh JK. Exerciseinduced
hypercalcemia and the calciotropic hormones. Journal
of Laboratory and Clinical Medicine 1985; 106: 229-232.
- 21. Inoue Y, Osawa T, Matsui A, Asai Y, Murakami Y et al. Changes
of serum mineral concentrations in horses during exercise.
Asian-Australian Journal of Animal Sciences 2002; 1: 531-536.
- 22. Kanungo S, Pradhan CR, Babu LK, Behera K, Palei AK et
al. Effect of periodicity of exercise on serum metabolites of
stall housed competition horses under climatic conditions of
Odisha. International Journal of Current Microbiology and
Applied Sciences 2018; 7: 3338-3347.
- 23. Rose RJ, Ilkiw JE, Arnold KS, Backhouse JW, Sampson
D. Plasma biochemistry in the horse during 3‐day event
competition. Equine Veterinary Journal 1980; 12: 132-136.
- 24. Soliman MK, Nadim MA. Calcium, sodium and potassium
level in the serum and sweat of healthy horses after strenuous
exercise. Zentralblatt für Veterinärmedizin A 1967; 14: 53-56.
- 25. Muñoz A, Riber C, Santisteban R, Lucas RG, Castejon FM.
Effect of training duration and exercise on blood‐borne
substrates, plasma lactate and enzyme concentrations in
Andalusian, Anglo‐Arabian and Arabian breeds. Equine
Veterinary Journal Supplements 2002; 34: 245-251.
- 26. Robert C, Goachet AG, Fraipont A, Votion DM, Van-Erck E
et al. Hydration and electrolyte balance in horses during an
endurance season. Equine Veterinary Journal Supplements
2010; 38: 98-104.
- 27. Manetta J, Brun JF, Maimoun L, Fedou C, Prefaut C et al.
The effects of intensive training on insulin-like growth factor
I and IGF binding proteins 1 and 3 in competitive cyclists:
relationships with glucose disposal. Journal of Sports Sciences
2003; 21: 147-154.
- 28. Ross R, Daqnone D, Jones PJ, Smith H, Paddags A et al.
Reduction in obesity and related comorbid conditions after
diet-induced weight loss or exercise-induced weight loss in
men. Annals of Internal Medicine 2000; 133: 92-103.
- 29. Graaf‐Roelfsema E, Van-Ginneken ME, Van-Breda E, Wijnberg
ID, Keizer HA et al. The effect of long‐term exercise on glucose
metabolism and peripheral insulin sensitivity in Standardbred
horses. Equine Veterinary Journal Supplements 2010; 38: 221-
225.
- 30. Kupczyński R, Śpitalniak K. Analysis of acid–base balance as
well as hematological and biochemical parameters in horses of
combined driving discipline. Archives Animal Breeding 2015;
58: 221-228.
- 31. Di Filippo PA, Martins LP, Meireles MAD, De Lannes ST.
Gender differences–induced changes in serum hematologic
and biochemical variables in Mangalarga Marchador horses
after a marcha gait competition. Journal of Equine Veterinary
Science 2016; 43: 18-22.