Ayşe Eser ELÇİN, Yaşar Murat ELÇİN, Zeynep AKTAŞ, Eylül AKPINAR, Ömer BEŞALTI, Pınar CAN

Subarachnoid transplantation of autologous neurogenically induced bone marrow derived mesenchymal stem cells for the treatment of fibrocartilaginous embolic myelopathy in two dogs

The objective of this study was to investigate the effects of autologous neurogenically induced bone marrow-derived mesenchymal stem cells (NIBM-MSCs) in two dogs suspected to have fibrocartilaginous embolic myelopathy (FCEM). Both dogs were paraplegic without deep pain perception (DPP) and tentatively diagnosed by MRI. Autologous NIBM-MSCs (5 × 106)were transplanted into the lumbar subarachnoid space two times with a 21-day interval for each patient. Based on a 21-month follow-up of the treated animals, the transplantation of NIBM-MSCs seems to be promising in subjects with FCEM lacking DPP.

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1. Cauzinille L. Fibrocartilaginous embolism in dogs. Vet Clin North Am Small Anim Pract 2000; 30: 155167.
2. Gandini G, Cizinauskas S, Lang J, Fatzer R, Jaggy A. Fibrocartilaginous embolism in 75 dogs: clinical findings and factors influencing the recovery rate. J Small Anim Pract 2003; 44: 7680.
3. De Risio L, Simon RP. Fibrocartilaginous embolic myelopathy in small animals. Vet Clin North Am Small Anim Pract 2010; 40: 859869.
4. Chung WH, Park SA, Lee JH, Chung DJ, Yang WJ, Kang EH, Choi CB, Chang HS, Kim DH, Hwang SH et al. Percutaneous transplantation of human umbilical cord-derived mesenchymal stem cells in a dog suspected to have fibrocartilaginous embolic myelopathy. J Vet Sci 2013; 14: 495497.
5. Levine GJ, Levine JM, Budke CM, Kerwin SC, Au J, Vinayak A, Hettlich BF, Slater MR. Description and repeatability of a newly developed spinal cord injury scale for dogs. Prev Vet Med 2009; 89: 121127.
6. Baykan E, Koc A, Elcin AE, Elcin YM. Evaluation of a biomimetic poly (ε-caprolactone)/β-tricalcium phosphate multispiral scaffold for bone tissue engineering: in vitro and in vivo studies. Biointerphases 2014; 9: 029011.
7. Lim JH, Boozer L, Mariani CL, Piedrahita JA, Olby NJ. Generation and characterization of neurospheres from canine adipose tissue-derived stromal cells. Cellular Reprogramming 2010; 12: 417425.
8. Abramson CJ, Garosi L, Platt SR, Dennis R, McConnell JF. Magnetic resonance imaging appearance of suspected ischemic myelopathy in dogs. Vet Radiol Ultrasound 2005; 46: 225229.
9. Nakamoto Y, Ozawa T, Katakabe K, Nishiya K, Yasuda N, Mashita T, Morita Y, Nakaichi M. Fibrocartilaginous embolism of the spinal cord diagnosed by characteristic clinical findings and magnetic resonance imaging in 26 dogs. J Vet Med Sci 2009; 71: 171176.
10. Grünenfelder FI, Weishaupt D, Green R, Steffen F. Magnetic resonance imaging findings in spinal cord infarction in three small breed dogs. Vet Radiol Ultrasound 2005; 46: 9196.
11. Donnelly EM, Lamanna J, Boulis NM. Stem cell therapy for the spinal cord. Stem Cell Research & Therapy 2012; 3: 24.
12. Penha EM, Meira CS, Guimarães ET, Mendonça MV, Gravely FA, Pinheiro CM, Pinheiro TM, Barrouin-Melo SM, Ribeiro-Dos-Santos R, Soares MB. Use of autologous mesenchymal stem cells derived from bone marrow for the treatment of naturally injured spinal cord in dogs. Stem Cells International 2014; 2014: 437521.
13. Park SS, Lee YJ, Lee SH, Lee D, Choi K, Kim WH, Kweon OK, Han HJ. Functional recovery after spinal cord injury in dogs treated with a combination of Matrigel and neural-induced adipose-derived mesenchymal stem cells. Cytotherapy 2012; 14: 584597.