Fatma ALKAN ATEŞ, Dilek ERDİKMEN OLGUN, Duygu TARHAN, Sinem ÜLGEN, Çağla YARAMIŞ PARKAN, Mehmet Erman OR, Ümit Bora BARUTÇU

Evaluation of tear and serum trace elements (copper, selenium, and cobalt) in sheep

Metabolic diseases such as enzootic ataxia, white muscle disease, and white liver disease are among the most important problems of livestock for sheep. The aim of this study was to analyze whether tears can be used as an alternative diagnostic method in determination of diseases that result from lack or excess of copper, selenium, and cobalt. Samples of serum and tears taken from 45 healthy sheep of the Kivircik breed aged between 2 and 4 years were analyzed. The concentrations of copper, selenium, and cobalt were measured in serum and tears as 118.0 ± 36.0, 58.0 ± 15.0, and 0.5 ± 0.3 μg/dL and 16.0 ± 8.0, 7.0 ± 4.0, and 2.0 ± 1.0 μg/dL, respectively, by using inductively coupled plasma optical emission spectrometry. The concentrations of copper and selenium in serum samples were significantly higher than those of tear samples (P < 0.001). However, cobalt concentrations in serum were found to be lower than those in tears (P < 0.001). As a result, the authors suggest that determining cobalt concentrations in tears may be a valuable alternative diagnostic method in the diagnosis of metabolic diseases of sheep, rather than determining the copper and selenium concentrations, which might not be as reliable.

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1. Ası T.Ta b lolarla Biyokimya. Ankara, Turkey: Nobel Tıp Kitapları Dağıtım (in Turkish).
2. Kaneko JJ, Harvey JW, Bruss ML.Clinical Biochemistry of Domestic Animals. 5th ed. New York, NY, USA: Academic Press; 1997. pp. 663694.
3. Haenlein GFW, Anke M. Mineral and trace element research in goats: a review. Small Ruminant Res 2011; 95: 219.
4. Hefnawy AEG, Tórtora-Pérez JL. The importance of selenium and the effects of its deficiency in animal health. Small Ruminant Res 2010; 89: 185192.
5. Schoster JV, Stuhr C, Kiorpes A. Tear copper and its association with liver copper concentrations in six adult sheep. Can J Vet Res 1995; 59: 129134.
6. Semeraro F, Costagliola C, Cancarini A, Gilberti E, Tosco E, Apostoli P. Defining reference values of trace elements in the tear film: diagnostic methods and possible applications. Ecotox Environ Safe 2012; 80: 190194.
7. Rantamaki AH, Seppanen-Laakso T, Oresic M, Jauhiainen M, Holopainen JM. Human tear fluid lipidome: from composition to function. PLoS One 2011; 6: e19553.
8. Zhao Z, Liu J, Wasinger VC, Malouf T, Nguyen-Khuong T, Walsh B, Willcox MDP. Tear lipocalin is the predominant phosphoprotein in human tear fluid. Exp Eye Res 2010; 90: 344349.
9. Ohashi Y, Dogru M, Tsubota K. Laboratory findings in tear fluid analysis. Clin Chim Acta 2006; 369: 1728.
10. Braun RJ. Dynamics of the tear film. Annu Rev Fluid Mech 2012; 44: 267297.
11. Dartt DA. Tear lipocalin: structure and function. Ocul Surf 2011; 9: 126138.
12. Nagyova B, Tiffany JM. Components responsible for the surface tension of human tears. Curr Eye Res 1999; 19: 411.
13. Kulovesi P, Telenius J, Koivuniemi A, Brezesinski G, Rantama A, Viitala T, Puukilainen E, Ritala M, Wiedmer SK, Vattulainen I et al. Molecular organization of tear fluid lipid layer. Biophys J 2010; 99: 25592567.
14. Janssen PT, van Bijsterveld OP. Origin and biosynthesis of human tear fluid proteins. Inves Ophthalmol Vis Sci 1983; 24: 623630.
15. de Souza GA, Godoy LM, Mann M. Identification of 491 proteins in the tear fluid proteome reveals a large number of proteases and protease inhibitors. Genome Biol 2006; 7: 72.
16. Shamsi FA, Chen Z, Liang J, Li K, Al-Rajhi AA, Chaudhry IA, Li M, Wu K. Analysis and comparison of proteomic profiles of tear fluid from human, cow, sheep, and camel eyes. Inves Ophthalmol Vis Sci 2011; 52: 91569165.
17. Simonsen LO, Harbak H, Bennekou P. Cobalt metabolism and toxicology-A brief update. Sci Total Environ 2012; 432: 210215.
18. Ayiannidis A, Giroussi S, Voulgaropoulos A. Aspects of protein bound copper in sheep plasma and its release in vitro especially after treatment with ammonium tetrathiomolybdate. J Trace Elements Med Biol 1996; 10: 245250.
19. Patching SG, Gardiner PH. Recent developments in selenium metabolism and chemical speciation: a review. J Trace Elements Med Biol 1999; 13: 193214.
20. Abou-Zeina HAA, Zaghawa AA, Nasr SM, HGE Keshta. Effects of dietary cobalt deficiency on performance, blood and rumen metabolites and liver pathology in sheep. Global Veterinaria 2008; 2: 182191.
21. Özkan B, Or ME. Ovine white liver disease. YYU Vet Fak Derg 2007; 18: 111114 (in Turkish with English abstract).
22. Sugen B, Gunes V. The importance of cardiac troponin kit analyses and serum enzyme activities in the diagnosis of heart muscle degeneration in lambs with white muscle disease. J Health Sci 2008; 17: 144149.
23. Chalmers GA. Swayback (enzootic ataxia) in Alberta lambs. Can J Comp Med 1974; 38: 111117.
24. Ağaoğlu ZT, Akgül Y, Bildik A. Study on the distribution of enzootic ataxia in Van and its environment. YYU Vet Fak Derg 1992; 3: 7190 (in Turkish with English abstract).
25. de Sousa IK, Hamad Minervino AH, Sousa Rdos S, Chaves DF, Soares HS, Barros Ide O, de Araujo CA, Junior RA, Ortolani EL. Copper deficiency in sheep with high liver iron accumulation. Vet Med Int 2012; 2012: 207950.