Some histocemical properties of the ceruminous glands in the meatus acusticus externus in cats and dogs

Bu çalışma dış kulak yolundaki seruminöz bezlerin bazı histokimyasal özelliklerini ortaya koymak amacıyla yapıldı. Çalışma materyali olarak çeşitli ırklardan 10 adet kedi ve 10 adet köpek kullanıldı. Kedi ve köpeklerde dış kulak yolundaki seruminöz bezlerin salgı özelliklerini belirlemek amacıyla; nötral mukosubstans ve glikojen için periyodik asit Schiff (PAS), asit mukosubstans için Alcian blue (pH 2,5) boyamaları yapıldı. Glikojen varlığı için diastase reaksiyonu uygulandı. Glikojen ve nötral mukosubstans için köpeklerde pozitif, kedilerde negatif reaksiyon gözlendi. Köpeklerdeki pozitif reaksiyonun glikojenden kaynaklanmadığı, diastazın negatif reaksiyon vermesi ile belirlendi. Asidik mukosubstans için Alcian blue (pH 2,5) kedilerde pozitif, köpeklerde negatif reaksiyon gözlendi.

Kedi ve köpeklerde dış kulak yolundaki serumen bezlerin bazı histokimyasal özellikleri

This study was conducted to determine some histochemicai properties of the ceruminous glands located in the meatus acusticus externus in cats and dogs. Ten cats and 10 dogs of various breeds were used as the study materials. To determine the properties of the secretions of the ceruminous glands in the meatus acusticus externus of cats and dogs, the periodic acid Schiff (PAS) stain was used for neutral mucosubstances and glycogen, while the Alcian blue stain (pH 2.5) was applied for the presence of acidic mucosubstances. The diastase reaction was applied to confirm the presence of glycogen. In dogs, positive staining was observed for glycogen and neutral mucosubstances, whereas, in cats, negative reactions were observed. The positive reactions observed in dogs were found not to originate from glycogen by the negative diastase reactions observed. - The Alcian blue stain (pH 2.5) for acidic mucosubstances gave positive reactions for cats and negative reactions for dogs.

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1. Junqueira, L.C., Cameiro, J., Kelley, R.O.: Basic Histology 9th ed. Appletion & Lange Stanford, Connecticut, 1998; 358-360.
2. Logas, D.B.: Diseases of the ear canal. Vet. Clin. North Am. Small Anim. Pract, 1994; 24: 905-919.
3. Augus, J.R.: Diseases of the ear canal. In the Complete Manuel of Ear Care. Lawrenceville, New Jersey, Veterinary Learning Systems. Inc., 1986.
4. Griffin, C: Histopathology of the external ear. In the Complete Manual of Ear Care. Lawrenceville, New Jersey, Veterinary Learning Systems, Inc., 1986.
5. Van der Gaag, I.: The pathology of the external ear canal in dogs and cats. Vet. Q., 1986; 8: 307-317.
6. Huang, H.P., Fixter, Ml., Little, C.J.L.: Lipid content of cerumen from normal dogs and otitic canine ears. Vet. Rec, 1994; 134: 380-381.
7. Chai, T.J., Chai, T.C.: Bactericidal activity of cerumen. Antimicrob. Agents Chemother., 1980; 18: 638-641.
8. Megarry, S., Pett, A., Scarlett, A., Teh, W., Zeigler, E., Canter, R.J.: The activity against yeast of human cerumen. J. Laryngol. Otol., 1988; 102:671-672
Gabal, M.A.: Preliminary studies on the mechanism of infection 15. and characterization of Malassezia pachydermatis in association with canine otitis externa. Mycopathologia, 1988; 104: 93-98.
10. August, J.R.: Otitis externa, a disease of multifactorial etiology. Vet. Clin. North Am. Small Anim. Pract, 1988; 18: 731.
11. Bergman, R.A., Afifi, A.K., Heidger, P.M.: Atlas of Microscopic Anatomy. Section 7- Integument, Iowa. 1999. 12. Fernando, S.D.A.: Microscopic Anatomy and Histochemistry of glands in the external auditory meatus of the cat (Felis domesticus). Am. J. Vet. Res., 1965; 26: 1157-1161. 13. Fernando, S.D.A.: A Histological and histochemical study of the glands of the external auditory canal of the dog. Res. Vet. Sci., 1966; 7: 116-119.
14. Woody, B.J., Fox, S.M.: Otitis externa: Seeing past the signs to discover the underlying course. Vet. Med., 1986; 81: 616-624. 15. Johnson, A., Hawke, M.: The non-auditory physiology of the external ear canal. In John AF Santos-Sacchi J(eds): Physiology of the Ear. New York, Raven Press., 1988; 41.
16. Sirigu, P., Cossu, M., Puxeddu, P., Marchisia, A.M., Perra, M.T.: Human ceruminous glands: a histochemical study. Basic Appl. Histochem., 1983; 27: 257-265.
17. Denk, H., Künzele, H., Plenk, H., Rüschoff, J., Seller, W.: Romeis Mikroskopische Technik. 17., neubearbeitete Auflage. Urban und Schwarzenberg, Munchen-Wien. Baltimore. 1989; 439-450.
18. Culling, C.F.A., Allison, R.T., Barr, W.D.: Cellular Pathology Technique. 4th ed., Butterworths, London. 1985; 214-255.
19. Bancroft, J.D., Gamble, M.: Theory and Practice of Histological Techniques. 5th ed. Churchill Livingstone. Toronto, 2002; 133-135.