Potential future applications of spermatheca extract from the marine snail Telescopium telescopium
Cytosol fraction of spermatheca and/or the ovotestis gland from the marine snail Telescopium telescopium was found to have antimicrobial, antiprotozoal, antifertility, and immunomodulation properties. The present experiment was conducted to explore the biochemical nature, composition, and pharmacological properties of the cytosol fraction along with its toxicity on the hematopoietic system in vivo. This glandular extract was analyzed and found to contain enzymes, hormones, minerals, vitamins, and other biologically active components like higher vertebrates. The present study showed that the extract was able to restrict the induced tumor (sarcoma-180) growth in mice concomitantly with an increased life span of the tumor bearing mice, but without any hematopoietic toxicity. This antitumor property was thought to be mediated immunologically as also found in other aspects of pharmacological actions of this extract; hence, it could be developed as a potent immunomodulator to be used for various pathological conditions in general, but in cancer in particular.
Potential future applications of spermatheca extract from the marine snail Telescopium telescopium
Cytosol fraction of spermatheca and/or the ovotestis gland from the marine snail Telescopium telescopium was found to have antimicrobial, antiprotozoal, antifertility, and immunomodulation properties. The present experiment was conducted to explore the biochemical nature, composition, and pharmacological properties of the cytosol fraction along with its toxicity on the hematopoietic system in vivo. This glandular extract was analyzed and found to contain enzymes, hormones, minerals, vitamins, and other biologically active components like higher vertebrates. The present study showed that the extract was able to restrict the induced tumor (sarcoma-180) growth in mice concomitantly with an increased life span of the tumor bearing mice, but without any hematopoietic toxicity. This antitumor property was thought to be mediated immunologically as also found in other aspects of pharmacological actions of this extract; hence, it could be developed as a potent immunomodulator to be used for various pathological conditions in general, but in cancer in particular.
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- Higa, T., Tanaka, I., Ikuko, I., Mursi Musman, 0., Roy, M.C., Kuroda, I.: Bioactive compounds from coral reef invertebrates. Pure Appl. Chem., 2001; 73: 589—593.
- Blunt, I.W., Copp, B.R., Munro, M.H.G., Northcote, P.T., Prinsep, M.R.: Marine natural products. Nat. Product Report, 2003; 20: 1—48.
- Pettit, G.R., Flahive, E.I., Boyd, M.R., Bai, R., Hamel, E., Pettit, R.K., Schmidt, ].M.: Antineoplastic agents 360. Synthesis and cancer cell growth inhibitory studies of Dolastatin 15 structural modiŞcations. Anticancer Drug Dis., 1998; 13: 47 —66.
- Lee, Y.S., Yang, H.O., Shin, K.H., Choi, H.S., Iung, S.H., Kim, Y.M., Oh, D.K., Linhardt, R.I., Kim, Y.S.: Suppression of tumor growth by a new glycosaminoglycan isolated from the African giant snail Achatina fulica. European I. Pharmacol., 2003; 465: 191— 198.
- Garcia—Rocha, M., Bonay, P., Avila, I.: The antitumoral compound Kahalalide F acts on cell lysosomes. Cancer Lett., 1996; 99: 43—50.
- Reese, M.T., Gulavita, N.K., Nakao, Y, Hamann, M.T., Yoshida, W.Y., Coval, S.I., Scheuer, PI.: Kulolide: a cytotoxic depsipeptide from a cephalaspidean mollusk, Philinopsis secicosa. I. Am. Chem. Soc., 1996; 118: 11081.
- Pakrashi, A., Roy, P., Datta, U.: Antimicrobial effects of protein(s) isolated from a marine mollusc Telescopium telescopium. Indian I. Physiol. Pharmacol, 2001; 45: 249-252.
- Pakrashi, A., Mukhopadhyay, S., Roy, P., Datta, U.: Effect of “SF— 50”, a partially puriŞed protein from spermatheca gland of a marine mollusc Telescopium telescopium on leishmania major. Science Culture, 2000; 66( 1 1— 12): 403-404.
- Pakrashi, A., Datta, U., Choudhury, A.: A search for immunocontraceptive agent from marine sources—role of antispermatheca globulin of Telescopium telescopium on fertility regulation in male rat. Indian I Experi Biol., 1992; 30: 1066-1074. different dimensions of pharmacological actions many of which from immunological pathways could be developed in the future as potent and effective immunomodulators against various disease conditions in general, and as an antitumor agent(s) in particular.
- Pakrashi, A., Datta, U.: In-vitro sperm agglutination and spermicidal activity of protein isolated from a marine mollusc Telescopium telescopium (Gastropod). Indian I Marine Sci., 2001; 30: 93—97.
- Datta, U., Roy, H., Pakrashi, A.: Effect of “SF—50”, a partially puriŞed protein from a marine gastropod mollusc Telescopium telescopium on female reproduction. Indian I Marine Sci., 2001; 30: 44—48.
- Datta, U.: Bioactive substance(s) from a marine mollusc Telescopium telescopium and its role on mammalian reproduction. PhD Dissertation. Iadavpur University. Kolkata, India. 2003.
- Laemmli, U.K.: Cleavage of structural proteins during the assembly at the head of bacteriophase- T—4. Nature, 1970; 227: 680—685.
- Sloneker, ].H. Methods in carbohydrate chemistry. New York, Academic Press, 1972.
- Auda, S.P., Vuthiganon, C., Elias, E.G.: Effects of different shortwave radiofrequencies on tumor growth. Cancer Res., 1980; 40: 2829—34.
- Mathe, G.: Active immunotherapy. Advances Cancer Res., 1971; 14: 1—36.
- McKhann, C.F., Iagarlamoody, S.M.: Manipulation of the immune responses towards immunotherapy of cancer. In: Membranes and viruses in immunopathology. New York, London, Academic Press, 1972.
- Morton, D.L.: Humoral tumor immunity in man: possible role in host defense against cancer. In: Membranes and Viruses in Immunopathology. New York, London, Academic Press. 1972.