Ochratoxin A-induced serum biochemical alterations in New Zealand white rabbits (Oryctolagus cuniculus)

Our objective was to study the effects of oral ochratoxin A (OT-A) intoxication in rabbits. New Zealand white rabbits of 8 weeks age, weighing 350-400 g, were utilized for the study. Rabbits were allotted randomly to 3 groups. Group 1 (6 animals) served as control. Groups 2 and 3 (12 animals each) were fed diets supplemented with 1000 and 2000 ppb OT-A, respectively, for 8 weeks. Blood samples were collected at weekly intervals from day 0 up to week 8 and sera were separated for biochemical analysis. Serum glucose and chloride levels showed marked progressive decrease whereas a marginal decrease was noticed in total protein and albumin. Serum levels of creatinine, and urea, and activities of aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase enzymes were increased in OT-A given groups. The effects were dose dependent. Globulins and albumin:globulin ratio was not affected significantly. SDS-PAGE revealed noticeable decrease in 81, 55-66, 48, and 44.5 KDa protein bands. Absence of 52 KDa proteins in the serum of rabbits fed 2000 ppb OT-A diets was noted. In conclusion, the observation of predominant serum biochemical alterations caused by 1000 and 2000 ppb OT-A in time and dose related fashion suggested progressive nephrotoxic and hepatotoxic effects in rabbits.

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Turk. J. Vet. Anim. Sci. 2010; 34: 525-531. Full text: pdf Other articles published in the same issue: Turk. J. Vet. Anim. Sci., vol.34, iss.6.

Ochratoxin A-induced serum biochemical alterations in New Zealand white rabbits (Oryctolagus cuniculus)

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Turk. J. Vet. Anim. Sci. 2010; 34: 525-531. Full text: pdf Other articles published in the same issue: Turk. J. Vet. Anim. Sci., vol.34, iss.6.,

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Ranjan, K.S., Sinha, A.K.: Occurrence of mycotoxigenic fungi and mycotoxins in animal feed from Bihar. Indian J. Sci. Fd. Agri., 1991; 56: 39-47. 2. Driehuis, F., Spanjer, M.C., Scholten, J.M., TeGiff el, M. C.: Occurrence of mycotoxins in feedstuff s of dairy cows and estimation of total dietary intakes. J. Dairy Sci., 2008; 91: 4261- 4271.
Dwivedi, P., Sharma, A.K., Singh, N.D.: Current status of mycotoxicosis in India and their control. Indian J. Vet. Pathol., 2008; 32: 217-225. 4. Marquardt, R.R., Frohlich, A.A.: A review of recent advances in understanding ochratoxicosis. J. Anim. Sci., 1992; 70: 3968- 3988.
Bennett, J.W., Klich, M.: Mycotoxins. Clin. Microbiol Rev., 2003; 16: 497-516 6. Kumar, A., Jindal, N., Shukla, C.L., Asrani, R.K., Ledoux, D.R., Rottinghaus, G.E.: Pathological changes in broiler chickens fed ochratoxin A and inoculated with Escherichia coli. Avian Pathol., 2004; 33: 413-417. 7. Dietrich, D.R., Heussner, A.H., O’Brien, E.: Ochratoxin A - Comparative pharmacokinetics and toxicological implications (experimental and domestic animals and humans). Fd. Add. Cont., 2005; 22(supp. 1): 45-52.
O’Brien, E., Dietrich, D.R.: Ochratoxin A - the continuing enigma. Critical Rev. Toxicol., 2005; 35: 33-60. 9. Elaroussi, M.A., Mohamed, F.R., El Barkouky, E.M., Atta, A.M., Abdou, A.M., Hatab, M.H.: Experimental ochratoxicosis in broiler chickens. Avian Pathol., 2006; 35: 263-269.
Pfohl-Leszkowicz1, A., Manderville, R.A.: Ochratoxin A: An overview on toxicity and carcinogenicity in animals and humans. Mol. Nutr. Food Res., 2007; 51: 61-99.
Prasanna, K., Sharma, A.K., Dwivedi, P., Satheesh, C.C., Telang, A.G.; Pathogenesis of ochratoxin A induced nephropathy in rats with special reference to oxidative damage, cytokine profi le and ultrastructural changes. Indian J. Vet. Pathol., 2007; 31: 1-10.
Sava, V., Velasquez, A., Song, S., Sanchez-Ramos, J.: Adult hippocampal neural stem/progenitor cells in vitro are vulnerable to the mycotoxin ochratoxin-A. Toxicol. Sci., 2007; 98: 187-197.
Bozzo, G., Ceci, E., Bonerba, I.E., Desantis, S., Tantillo, G.: Ochratoxin A in laying hens: high-performance liquid chromatography detection and cytological and histological analysis of target tissues. J. Appl. Poult. Res., 2008; 17: 151-156.
Elaroussi, M.A., Mohamed, F.R., Elgendy, M.S., El Barkouky, E.M., Abdou, A.M., Hatab, M.H.: Ochratoxicosis in broiler chickens: functional and histological changes in target organs. Int. J. Poult. Sci., 2008; 7: 414-422.
Stec, J., Rachubik1, J., Szczotka, M., Kuźmak, J.: Eff ects of penicillium mycotoxins: citrinin, ochratoxin A, and patulin on in vitro proliferation of bovine lymphocytes. Bull. Vet. Inst. Pulawy., 2008; 52: 163-167.
Wilk–Zasadna, I., Minta, M.: Developmental toxicity of ochratoxin A in rat embryo midbrain micromass cultures. Int. J. Mol. Sci., 2009; 10: 37-49.
Mir, M.S., Dwivedi, P., Charan, K.: Ochratoxin – A induced acute toxicity in rabbits. Indian J. Vet. Pathol., 1999; 23: 8-13.
Mir, M.S., Dwivedi, P.: Clinical studies on experimental subacute ochratoxicosis in rabbits. Indian J. Vet. Pathol., 2000; 24: 99-101.
Wangikar, P.B., Dwivedi, P., Sinha, N.: Teratogenic eff ects of ochratoxin A in rabbits. Wld. Rabbit Sci., 2004; 12: 159-171.
Wangikar, P.B., Dwivedi, P., Sinha, N., Sharma, A.K., Telang, A.G.: Teratogenic eff ects in rabbits of simultaneous exposure to ochratoxin A and afl atoxin B1 with special reference to microscopic eff ects. Toxicology, 2005, 215: 37-47
Mir, M.S., Dwivedi, P.: Ochratoxin-A Induced Nephrotoxicity in Rabbits. Second J & K Science Congress, 25-27 July 2006, University of Kashmir, Srinagar, Kashmir (J&K). 2006; Ab VET-7: 26-27.
Mir, M.S., Dwivedi, P.: Residue dynamics and built-up of ochratoxin-A following sub-acute intoxication in rabbits. Second J & K Science Congress, 25-27 July 2006, University of Kashmir, Srinagar, Kashmir (J&K). 2006; Ab.VET-8: 27.
Kumar, M., Dwivedi, P., Sharma, A.K. Singh, N.D., Patil, R.D.: Haematological changes induced by Ochratoxin A and citrinin alone and in combination in young growing rabbits. Indian J. Vet. Pathol., 2007; 31: 45-49.
Mir, M.S., Dwivedi, P.: Haematological alterations in New Zealand White rabbits (Oryctolagus cuniculus) due to subacute ochratoxicosis-A. SKUAST J. Res., 2008; 10: 242-250.
Mir, M.S., Dwivedi, P.: Pathology of induced ochratoxicosis-A in rabbits (Oryctolagus cuniculus). SKUAST J. Res., 2008; 10: 99-111.
Mir, M.S., Dwivedi, P.: Immunopathology of Ochratoxicosis - A in New Zealand White Rabbits (Oryctolagus cuniculus). Vet Scan, 2010, 5(1): Article 54.
Trenk, F.L., Butz, M.E., Chu, F.S.: Production of ochratoxins in diff erent cereal products by Aspergillus ochraceus. Appl. Microbiol., 1971; 21: 1032-1035.
Davis, B.J.: Disc gel electrophoresis II. Method and application to human serum proteins. Ann. N.Y. Acad. Sci., 1964; 121: 404- 427.
Snedecor, G.W., Cochran, W.G.: Statistical Methods, 8th edn., 1968; Iowa State Univ. Press, Ames.
Kumar, M., Dwivedi, P., Sharma, A.K., Telang, A.G., Patil, R.D., Singh, N.D., Sangar, M.: Ochratoxin-A and citrinin induced biochemical changes in New Zealand White rabbits. Indian J. Vet. Pathol., 2007; 31: 135-139.
Sivakumar, G., Dwivedi, P., Sharma, A.K., Kumar, M., Nimalesan, S.: Fumonisin B1 and ochratoxin A induced biochemical changes in young male New Zealand White rabbits. Indian J. Vet. Pathol., 2009; 33: 30-34.
Stoev, S., Kunev, I., Radic, B.: Haematological, biochemical and toxicological investigations in spontaneous cases of mycotoxic nephropathy (ochratoxicosis) in pigs. Bulgarian J. Agri. Sci., 1997; 3: 507-516.
Raina, J.S., Roy, K.S., Balwant Singh: Biochemical and Histochemical studies in experimental mycotoxicosis in chicks. Indian J. Anim. Sci., 1991; 61: 1276-1281.
Purchase, I.F.H., Th eron, J.J.: Th e acute toxicity of ochratoxin A to rats. Fd. Cosmet. Toxicol., 1968; 6: 479-483.
Kumar, M., Dwivedi, P., Sharma, A.K. Singh, N.D., Patil, R.D.: Ochratoxin A and citrinin nephrotoxicity in New Zealand White rabbits: an ultrastructural assessment. Mycopathologica, 2007; 163: 21-30.
McLaughlin, J., Philip J. Padfi eld, P.J., Julian P. H. Burt, J.P.H., Catherine A. O’Neill, C.A.: Ochratoxin A increases permeability through tight junctions by removal of specifi c claudin isoforms. Am J Physiol Cell Physiol., 2004; 287: C1412-C1417.
Agawane, S.B., Lonkar, P.S.: Eff ect of probiotic containing Saccharomyces on experimental ochratoxicosis in broilers: Hematobiochemical studies. J. Vet. Sci., 2004; 5: 359-367.
Creppy, E.E., Lungnier, A.A.J., Beck, G., Roschenthaler, R., Dirheimer, G.: Action of ochratoxin A on cultured hepatoma cells: Reversion of inhibition by phenylalanine. FEBS Lett., 1979; 104: 287-290.
Garcia, A.R., Avila, E., Rosiles, R., Petrone, V.M.: Evaluation of two mycotoxin binders to reduce toxicity of broiler diets containing ochratoxin A and T-2 toxin contaminated grain. Avian Dis., 2003; 47: 691-699
Kumar, A., Jindal, N., Shukla, C.L., Pal, Y., Ledoux, D.R., Rottinghaus, G.E.: Eff ect of ochratoxin A on Escherichia coli - challenged broiler chicks. Avian Dis., 2003; 47: 415-424.
Th acker, H.L., Carlton, W.W.: Ochratoxin-A mycotoxicosis in the guinea pigs. Fd. Contaqm. Toxicol., 1977; 15: 563-574.
Dwivedi, P., Burns, R.B.: Th e natural occurrence of ochratoxin-A and its eff ects in poultry. A review. Part-I. Epidemiology and toxicity. Wlds’ Poult. Sci. J., 1986; 42: 32-47.