Pathological, immunohistochemical, and bacteriological findings in the mammary glands and supramammary lymph nodes of cows with a history of abortion due to Brucella abortus
Bu çalışmada, Brucella abortus’a bağlı atık yapmış ineklerin meme bezi ve supramammar lenf düğümleri patolojik, immunohistokimyasal ve bakteriyolojik olarak değerlendirildi. Çalışma materyalini, ilk gebeliklerinde atık yapmış Brucella-seropozitif 11 inek oluşturdu. Histopatolojik olarak, meme bezlerinde lenfo-plazmositik ve histiyositik intersitisyel mastitis gözlendi. Lenf düğümlerinde folliküler hiperplazi ve medullar plazmasitozis görüldü. İmmunohistokimyasal olarak, 3 ineğin meme bezinde intralobular intersitisyum ve periduktal stromada özellikle makrofaj ve nötrofil lökositlerin sitoplazmasında brucella antijeni tespit edildi. Ayrıca, alveolar lumende deskuamatif alveolar epitelyumun şiddetli immunopozitif sitoplazmik reaksiyonu gözlendi. Lenf düğümlerinde, antijen bulunan makrofajlara sıklıkla medullar bölgede rastlandı. Dört ineğin meme bezi ve supramammar lenf düğümlerinden B. abortus biyotip 3 izole edildi. Brusellozisin kesin tanısında en güvenilir yöntem etken izolasyonu olmakla beraber, bakteriyel kültürün negatif olduğu veya dokunun formalin ile tespit edildiği durumlarda, brucella antijenlerinin ortaya konulması için immunohistokimyasal yöntemlerin teşhis aracı olarak kullanılabileceği sonucuna varılmıştır.
Brucella abortus’a bağlı atık yapmış ineklerin meme bezi ve supramammar lenf düğümlerinde patolojik, immunohistokimyasal ve bakteriyolojik bulgular
The present study examined the mammary glands and supramammary lymph nodes of cows with a history of abortion due to Brucella abortus, and presents the histopathological, immunohistochemical, and bacteriological results. The study included 11 Brucella-seropositive cows that aborted during their first gestation. Histopathologically, the mammary glands showed lymphoplasmacytic and histiocytic interstitial mastitis. The lymph nodes had lymphofollicular hyperplasia and medullary plasmacytosis. Immunohistochemistry results showed brucellar antigens, predominantly in the cytoplasm of macrophages, and neutrophils in the intralobular interstitium and periductal stroma in the udders of 3 cows. Desquamated alveolar epithelium also indicated intense immunopositivity. In the lymph nodes, macrophages containing the antigen were frequently observed in the medullary region. B. abortus biotype 3 was isolated from the udders and supramammary lymph nodes of 4 cows. Although the most specific procedure for diagnosing the disease is isolation of the causative organism, for suspected cases in which the bacteriologic culture is negative or the material is fixed in formalin, immunohistochemistry may be used as a diagnostic tool for the detection of Brucella organisms.
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