Dauda Mohammed GONI, Saleha ABDULAZIZ, Gurmeet Kaur DHALIWA, Zunita ZAKARIA, Ibrahim Jalo MUHAMMAD, Mohamed Abdelrahman MOHAMED, Abdulrasheed Aliyu BELLO, Asinamai Athliamai BITRUS

Occurrence of Arcobacter in dogs and cats in Selangor,Malaysia, and associated risk factors

Arcobacter is widely regarded as an emerging foodborne pathogen because of its increasing occurrence in food production causing gastroenteritis in humans. In addition, it is also reported as a potential zoonosis. The objectives of this study were to determine the occurrence of Arcobacter in dogs and cats and the associated risk factors. Rectal and buccal cavity swab samples were taken from dogs (n = 40) and cats (n = 40) owned by individuals who brought the animals to a university teaching hospital and stray dogs (n = 61) and cats (n = 46). Suspected colonies of Arcobacter were subjected to biochemical tests (catalase, oxidase, and hippurate hydrolysis and indoxyl acetate hydrolysis tests). Multiplex polymerase chain reaction (mPCR) was employed for the confirmation and differentiation of the isolates. Results showed that the rates of Arcobacter carriage were 34.8% and 45.0% in stray and pet cats, respectively, while in stray and pet dogs the occurrences were detected at 50.8% and 60.0%, respectively. Arcobacter butzleri was the only species identified. The risk factors for Arcobacter infections in dogs and cats were determined through a questionnaire and analyzed statistically. The factors that were found to significantly increase the risk of Arcobacter infection were households with multiple pets and the source of drinking water.

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1. Vandamme P, Pugina P, Benzi G, Van Etterijck R, Vlaes L, Kersters K, Lauwers S. Outbreak of recurrent abdominal cramps associated with Arcobacter butzleri in an Italian school. J Clin Microbiol 1992; 30: 2335-2337.
2. Shah A, Saleha A, Zunita Z, Cheah Y, Murugaiyah M, Korejo N. Genetic characterization of Arcobacter isolates from various sources. Vet Microbiol 2012; 160: 355-361.
3. Öngör H, Çetinkaya B, Açik M, Atabay H. Investigation of arcobacters in meat and faecal samples of clinically healthy cattle in Turkey. Lett Applied Microbiol 2004; 38: 339-344.
4. De Smet S, De Zutter L, Van Hende J, Houf K. Arcobactercontamination on pre- and post-chilled bovine carcasses and in minced beef at retail. J Appl Microbiol 2010; 108: 299-305.
5. Patyal A, Rathore R, Mohan H, Dhama K, Kumar A. Prevalence of Arcobacter spp. in humans, animals and foods of animal origin including sea food from India. Transbound Emerg Dis 2011; 58: 402-410.
6. Shah A, Saleha A, Zunita Z, Murugaiyah M. ArcobacterAn emerging threat to animals and animal origin food products? Trends Food Sci Technol 2011; 22: 225-236.
7. Teague NS, Srijan A, Wongstitwilairoong B, Poramathikul K, Champathai T, Ruksasiri S, Mason CJ. Enteric pathogen sampling of tourist restaurants in Bangkok, Thailand. J Travel Med 2010; 17: 118-123.
8. Jiang ZD, DuPont HL, Brown EL, Nandy RK, Ramamurthy T, Sinha A, Rodriques S. Microbial etiology of travelers diarrhea in Mexico, Guatemala, and India: importance of enterotoxigenic Bacteroides fragilis and Arcobacter species. J Clin Microbiol 2010; 48: 1417-1419.
9. Taylor DN, Kiehlbauch JA, Tee W, Pitarangsi C, Echeverria P. Isolation of group 2 aerotolerant Campylobacter species from Thai children with diarrhea. J Infect Dis 1991; 163: 1062-1067.
10. Fera M, La Camera E, Carbone M, Malara D, Pennisi M. Pet cats as carriers of Arcobacter spp. in Southern Italy. J Appl Microbiol 2009; 106: 1661-1666.
11. Ho HT, Lipman LJ, Gaastra W. Arcobacter, what is known and unknown about a potential foodborne zoonotic agent! Vet Microbiol 2006; 115: 1-13.
12. Samie A, Obi C, Barrett L, Powell S, Guerrant R. Prevalence of Campylobacter species, Helicobacter pylori and Arcobacterspecies in stool samples from the Venda region, Limpopo, South Africa: studies using molecular diagnostic methods. J Infection 2007; 54: 558-566.
13. Abdelbaqi K, Buissonnière A, Prouzet-Mauleon V, Gresser J, Wesley I, Mégraud F, Menard A. Development of a real-time fluorescence resonance energy transfer PCR to detect Arcobacter species. J Clin Microbiol 2007; 45: 3015-3021.
14. Adesiji Y, Oloke J. Detection of Arcobacters from faeces of healthy pigs in Osogbo, south western Nigeria. Tropical Veterinarian 2009; 27: 28-35.
15. Miller WG, Wesley IV, On SL, Houf K, Mégraud F, Wang G, Mason CJ. First multi-locus sequence typing scheme for Arcobacter spp. BMC Microbiol 2009; 9: 196.
16. Collado L, Guarro J, Figueras MJ. Prevalence of Arcobacter in meat and shellfish. J Food Prot 2009; 72: 1102-1106.
17. Fernández H, Krause S, Paz Villanueva M. Arcobacter butzleri an emerging enteropathogen: communication of two cases with chronic diarrhea. Braz J Microbiol 2004; 35: 216-218.
18. Aydin F, Gümüşsoy K, Atabay H, Iça T, Abay S. Prevalence and distribution of Arcobacter species in various sources in Turkey and molecular analysis of isolated strains by ERIC‐PCR. J Appl Microbiol 2007; 103: 27-35.
19. Houf K, De Smet S, Baré J, Daminet S. Dogs as carriers of the emerging pathogen Arcobacter. Vet Microbiol 2008; 130: 208-213.
20. Petersen RF, Harrington CS, Kortegaard HE, On S. A PCR‐DGGE method for detection and identification of Campylobacter, Helicobacter, Arcobacter and related Epsilobacteria and its application to saliva samples from humans and domestic pets. J Appl Microbiol 2007; 103: 2601-2615.
21. Snelling W, Matsuda M, Moore J, Dooley J. Under the microscope: Arcobacter. Lett Appl Microbiol 2006; 42: 7-14.
22. Prouzet-Mauléon V, Labadi L, Bouges N, Menard A, Megraud F. Arcobacter butzleri:underestimated enteropathogen. Emerg Infect Dis 2006; 12: 307.
23. Atabay HI, Corry JE. Evaluation of a new arcobacter enrichment medium and comparison with two media developed for enrichment of Campylobacter spp. Int J Food Microbiol 1998; 41: 53-58.
24. Houf K, Tutenel A, De Zutter L, Van Hoof J, Vandamme P. Development of a multiplex PCR assay for the simultaneous detection and identification of Arcobacter butzleri, Arcobacter cryaerophilus and Arcobacter skirrowii. FEMS Microbiol Lett 2000; 193: 89-94.
25. Amare L, Saleha A, Zunita Z, Jalila A, Hassan L. Prevalence of Arcobacter spp. on chicken meat at retail markets and in farm chickens in Selangor, Malaysia. Food Control 2011; 22: 732-736.