Mürsel DEMİREL, İbrahim TAŞAL, Ahmet SEMACAN

Kısraklarda $PGF2alpha$ enjeksiyonları sonrası östrus, ovulasyon ve gebelik oranları

Prostaglandin $F2alpha$ kısraklarda senkronizasyon amacıyla yaygın olarak kullanılmaktadır. Senkronizasyon amacıyla kullanılan tek ve çift doz $PGF2alpha$ 'nın östrus, ovulasyon ve gebelik oranları üzerindeki etkisinin belirlenmesi amacıyla 4-17 yaşlarında 43 adet Saf Kan İngiliz ve Yarım Kan İngiliz kısrak kullanıldı. Otuz Kısrağa östrus siklusunun dönemine bakılmaksızın senkronizasyon için 10 mg. $PGF2alpha$ (Dinoprost Tromethamine, Dinolytic) i.m enjekte edildi. Enjeksiyon gününden itibaren günlük aygır muayenesi ve gün aşırı rektai muayeneyle östrus belirtileri ve foliküler gelişmeler incelendi ve östrus süresince günlük rektai muayeneyle ovuiasyon zamanı belirlendi. Östrusta olanlara (n: 11) östrus süresince gün aşırı doğal aşım veya taze sperma ile sun'i tohumlama uygulandı. İlk enjek¬siyon sonrası östrusta olmayan kısraklara (n: 19) 14 gün sonra ikinci enjeksiyon yapıldı ve ilk enjeksiyondaki tüm uygulamalar tekrarlandı. Kontrol grubundaki kısraklar (n: 13), $PGF2alpha$ enjekte edilmeyen ve östrus süresince gün aşırı doğal aşım ya da taze sperma ile sun'i tohumlama uygulananlar arasından seçildi. Gebelik tanısı amacıyla tüm kısraklara ovuiasyon sonrası 30-35. günlerde ve 60. günde rektal muayene yapıldı. Tek ve çift doz $PGF2alpha$ enjekte edilen kısraklarda ve kontrol grubundakilerde östrus, ovuiasyon ve gebelik oranları sırasıyla, %36.66, %33.33 ve %45.45-, %68.42, %78.94 ve %44.66; %100, %92.30 ve %53.84 olarak belirlendi. Sonuç olarak, $PGF2alpha$ enjeksiyonunun östrus, ovulasyon ve gebelik oranlan üzerinde olumsuz bir etkisinin olmaması nedeniyle senkronizasyon amacıyla kullanılabileceği ancak, tek doz enjeksiyonda östrus ve ovuiasyon oranlarının düşük olmasından dolayı çift doz enjeksiyonların daha yararlı olacağı kanısına varılmıştır.

Estrous, ovulation and pregnancy rates following $PGF2alpha$ injection in mares

Prostaglandin $F2alpha$ was commonly used for synchronisation in mares. The purpose of this study was to see the effect of single and double doses of $PGF2alpha$ on estrous, ovulation and pregnancy rates. Therefore 43 Thoroughbred mares, between 4-17 years old, were used. Thirty mares 10 mg $PGF2alpha$ (Dinoprost Tromethamine, Dinolytic) was injected intramuscularly, regardless of the stage of the estrous cycle, for synchronization. Estrous behaviors and follicular development were examined with daily teasing and rectal palpation every other day, after $PGF2alpha$ injection and ovulation times determined daily rectal palpation during estrous. The mares which in estrous (n: 11) natural service or artificial insemination with fresh semen were done every other day until estrous behavior lasted. The mares (n: 19), were not estrous after first $PGF2alpha$ injection, were injected second PGF20114 days later and repeated all applications after first PGF20: injections. Control group (n: 13) were chosen from the mares which were not injected $PGF2alpha$ in estrous and natural service or artificial insemination with fresh semen were done every other day until estrous behavior lasted. For pregnancy diagnosis, rectal palpation were done all mares between 30-35th and 60th days after ovulation. Estrous, ovulation and pregnancy rates obtained from first or second $PGF2alpha$ injected mares and control group were 36.66%, 33.33% and 45.45%; 68.42%, 78.94% and 46.66; 100%, 92.30% and 53.84%, respectively.Consequently, because of no detrimental effect of $PGF2alpha$ injections on estrous, ovulation and pregnancy rates, it was concluded that PGF20C injections for synchronization but, estrous and ovulation rates low in single dose injection therefore double doses injections would be more beneficial.

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