Two experiments were conducted to determine the in vitro solubility of dry matter and crude protein of wet feed and dry feed of a commercial broiler diet. In the first experiment, to two g of feed samples of a commercial broiler pelleted diet was added 0-dry (80 g/kg water content) and 1.5 g water per g of feed (640 g/kg water content), and they were then incubated at one of two different levels of pepsin-HCl solutions, low (48 mg pepsin in 2 ml 0.1M HCl), or high (64 mg pepsin in 3 ml 0.1M HCl) for 0, 15, 30, 60, 120, 180, and 240 minutes at 42ºC. In the second experiment, to 2 g of a commercial broiler feed was added 0-dry (80 g/kg water content), 0.2, 0.4, 0.6, 0.8, 1.0, 1.5, 1.8, and 2.0 g water per g of dry feed (240, 340, 430, 480, 540, 580, 630, and 700 g/kg water content, respectively). To each feed sample was also added 64 mg pepsin with 3 ml-HCl (the high level from experiment 1), and then incubated for 10, 20, 30, 60, 180 and 240 minutes at 42ºC. After filtration and drying processes, dry matter and crude protein analyses were done for each sample. In experiment 1, wetting the feed with 1.5 g water per g feed followed by incubation in pepsin-HCl solution increased both dry matter solubility and crude protein solubility. There were also higher solubilities of feed nutrient with a high concentration of pepsin-HCL with a than low concentration of pepsin-HCL. In experiment 2, dry feed samples (80 g/kg water content) and feed samples with 240 and 340 g/kg water content had similar nutrient solubility, but their solubility values were significantly (P

Two experiments were conducted to determine the in vitro solubility of dry matter and crude protein of wet feed and dry feed of a commercial broiler diet. In the first experiment, to two g of feed samples of a commercial broiler pelleted diet was added 0-dry (80 g/kg water content) and 1.5 g water per g of feed (640 g/kg water content), and they were then incubated at one of two different levels of pepsin-HCl solutions, low (48 mg pepsin in 2 ml 0.1M HCl), or high (64 mg pepsin in 3 ml 0.1M HCl) for 0, 15, 30, 60, 120, 180, and 240 minutes at 42ºC. In the second experiment, to 2 g of a commercial broiler feed was added 0-dry (80 g/kg water content), 0.2, 0.4, 0.6, 0.8, 1.0, 1.5, 1.8, and 2.0 g water per g of dry feed (240, 340, 430, 480, 540, 580, 630, and 700 g/kg water content, respectively). To each feed sample was also added 64 mg pepsin with 3 ml-HCl (the high level from experiment 1), and then incubated for 10, 20, 30, 60, 180 and 240 minutes at 42ºC. After filtration and drying processes, dry matter and crude protein analyses were done for each sample. In experiment 1, wetting the feed with 1.5 g water per g feed followed by incubation in pepsin-HCl solution increased both dry matter solubility and crude protein solubility. There were also higher solubilities of feed nutrient with a high concentration of pepsin-HCL with a than low concentration of pepsin-HCL. In experiment 2, dry feed samples (80 g/kg water content) and feed samples with 240 and 340 g/kg water content had similar nutrient solubility, but their solubility values were significantly (P