Growth performance and intestinal histomorphology in egg-type growing roosters fed recycled food waste containing effective microorganisms
To evaluate the effects of a recycled food waste (eco-feed) containing effective microorganisms derived from Japanese mugwort silage juice (EJP) on growth performance and intestinal histomorphology, 48 male chickens of 14 days old were divided into 0%, 20%, 40%, and 60% EJP diet groups. At 20 weeks of age, body weight gain was similar in the 20% EJP group but lower in the 40% and 60% EJP groups (P < 0.05) compared to that in the control group. The relative total intestinal length and weight tended to be long and heavy, respectively, in the 20% and 40% EJP groups, but short and light, respectively, in the 60% EJP group. Although numerous villi were found in the jejunum and ileum of all EJP groups, the villus height and size observed in these groups were smaller. Cell area and cell mitosis number in the duodenum and jejunum of all EJP groups were lower than those of the control group (P < 0.01). Epithelial cells on the villus apical surface of the 20% EJP group showed morphology similar to that of cells on the villus apical surface of the control group in all of the intestinal segments. The growth performance data suggested that EJP could be incorporated at up to a level of 20% in chicken diets. The intestinal histological results suggested that long-term feeding of an EJP diet at up to the 40% level may result in a long and heavy intestine by increasing the numbers of miniature intestinal villi.
Growth performance and intestinal histomorphology in egg-type growing roosters fed recycled food waste containing effective microorganisms
To evaluate the effects of a recycled food waste (eco-feed) containing effective microorganisms derived from Japanese mugwort silage juice (EJP) on growth performance and intestinal histomorphology, 48 male chickens of 14 days old were divided into 0%, 20%, 40%, and 60% EJP diet groups. At 20 weeks of age, body weight gain was similar in the 20% EJP group but lower in the 40% and 60% EJP groups (P < 0.05) compared to that in the control group. The relative total intestinal length and weight tended to be long and heavy, respectively, in the 20% and 40% EJP groups, but short and light, respectively, in the 60% EJP group. Although numerous villi were found in the jejunum and ileum of all EJP groups, the villus height and size observed in these groups were smaller. Cell area and cell mitosis number in the duodenum and jejunum of all EJP groups were lower than those of the control group (P < 0.01). Epithelial cells on the villus apical surface of the 20% EJP group showed morphology similar to that of cells on the villus apical surface of the control group in all of the intestinal segments. The growth performance data suggested that EJP could be incorporated at up to a level of 20% in chicken diets. The intestinal histological results suggested that long-term feeding of an EJP diet at up to the 40% level may result in a long and heavy intestine by increasing the numbers of miniature intestinal villi.
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- Turhan, Ş., Özbag, B.C., Çetin, B.: Analysis of the Turkish broiler sector: the case of price competition. New Zeal. J. Agr. Res., 2006; 49: 431-437.
- Westendorf, M.L., Dong, Z.C., Schoknecht, P.A.: Recycled cafeteria food waste as a feed for swine: nutrient content, digestibility, growth and meat quality. J. Anim. Sci., 1998; 76: 2976-2983. 3. Horinouchi, S., Misumi, H., Iwakiri, M., Irie, M., Takahashi, T., Morita, T., Tateishi, M.: Production of high quality pork by using ecofeed. Bull. Miyazaki Lives. Exp. Stn., 2007; 68-74. 4. Murano, T., Aoki, D.: Utilization of high protein and high lipid ecofeed replaced feed on the laying hens. Bull. Chiba Prefect. Lives. Res. Cent., 2009; 9-12.
- Apata, D.F.: Growth performance, nutrient digestibility and immune response of broiler chicks fed diets supplemented with a culture of Lactobacillus bulgaricus. J. Sci. Food Agric., 2008; 88: 1253-1258. 6. Li, L., Hou, Z.P., Li, T.J., Wu, G.Y., Huang, R.L., Tang, Z.R., Yang, C.B., Gong, J., Yu, H., Kong, X.F., Pan, E., Ruan, Z., Xhu, W.Y., Deng, Z.Y., Xie, M., Deng, J., Yin, F.G., Yin, Y.L.: Eff ects of dietary probiotic supplementation on ileal digestibility of nutrients and growth performance in 1- to 42-day-old broilers. J. Sci. Food Agric., 2008; 88: 35-42.
- Yu, B., Liu, J.R., Hsiao, F.S., Chiou, P.W.S.: Evaluation of Lactobacillus reuteri Pg4 strain expressing heterologous β-glucanase as a probiotic in poultry diets based on barley. Anim. Feed Sci. Technol., 2008; 141: 82-91.
- Higgins, S.E., Higgins, J.P., Wolfenden, A.D., Henderson, S.N., Torres-Rodriguez, A., Tellez, G., Hargis, B.: Evaluation of a Lactobacillus-based probiotic culture for the reduction of Salmonella enteritidis in neonatal broiler chicks. Poult. Sci., 2008; 87: 27-31. 9. Incharoen, T., Yamauchi, K., Th ongwittaya, N.: Intestinal villus histological alterations in broilers fed dietary dried fermented ginger. J. Anim. Physiol. Anim. Nutr., 2010; 94(5): e130-e137.
- Meimandipour, A., Hair-Bejo, M., Shuhaimi, M., Azhar, K., Soleimani, A.F., Rasti, B., Yazid, A.M.: Gastrointestinal tract morphological alteration by unpleasant physical treatment and modulating role of Lactobacillus in broilers. Poult. Sci., 2010; 51: 52-59.
- Yamauchi, K., Incharoen, T., Yamauchi, K.: Th e relationship between intestinal histology and function as shown by compensatory enlargement of remnant villi aft er midgut resection in chickens. Anat. Rec., 2010; 293: 2071-2079.
- SAS Institute: SAS/STAT User’s Guide (Release 6.03). SAS Institute Inc., Cary, North Carolina, 1998.
- Mountzouris, K.C., Tsitrsikos, P., Palamidi, I., Arvaniti, A., Mohnl, M., Schatzmayr, G., Fegeros, K.: Eff ects of probiotic inclusion levels in broiler nutrition on growth performance, nutrient digestibility, plasma immunoglobulins, and cecal microfl ora composition. Poult. Sci., 2010; 89: 58-67.
- Zhou, X., Wang, Y., Gu, Q., Li, W.: Eff ect of dietary probiotic, Bacillus coagulans, on growth performance, chemical composition, and meat quality of Guangxi Yellow chicken. Poult. Sci., 2010; 89: 588-593.
- Caspary, W.F.: Physiology and pathophysiology of intestinal absorption. Am. J. Clin. Nutr., 1992; 55: 299s-308s.
- Tarachai, P., Yamauchi, K.: Eff ects of luminal nutrient absorption, intraluminal physical stimulation, and intravenous parenteral alimentation on the recovery responses of duodenal villus morphology following feed withdrawal in chickens. Poult. Sci., 2000; 79: 1578-1585.
- Maneewan, B., Yamauchi, K.: Intestinal villus recovery in chickens refed semi-purifi ed protein-, fat-, or fi bre-free pellet diets. Br. Poult. Sci., 2004; 45: 163-170.
- Wiseman, J.: Feeding of Non-Ruminant Livestock. Butterworths, London. 1987.
- Wissig, S.L., Graney, D.O.: Membrane modifi cations in the apical endocytic complex of ileal epithelial cells. J. Cell Biol., 1968; 39: 564-579.
- Isshiki, Y., Nakahiro, Y., Zhou, Z.X.: Feed digestibility in diff erent intestinal parts of chickens. Japanese J. Zootech. Sci., 1989; 60: 1082-1092.
- Imondi, A.R., Bird, F.H.: Th e sites of nitrogen absorption from the alimentary tract of the chicken. Poult. Sci., 1965; 44: 916- 920.
- Yamauchi, K., Kamisoyama, H., Isshiki, Y.: Eff ects of fasting and refeeding on structures of the intestinal villi and epithelial cells in White Leghorn hens. Br. Poult. Sci., 1996; 37: 909-921.
- Noy, Y., Sklan, D.: Digestion and absorption in the young chick. Poult. Sci., 1995; 74: 366-373.
- Lenhardt, L., Mozes, S.: Morphological and functional changes of the small intestine in growth-stunted broilers. Acta Vet. Brno, 2003; 72: 353-358.