Estimation of Genetic Distance in Meat and Layer Pure Lines Using Randomly Amplified Polymorphic DNA

The purpose of this study was to evaluate genetic similarities and distances among pure lines of poultry using the RAPD technique. In this study four layer and three meat type pure lines were used. Venous blood samples from five males and five females of each line were collected from the venae cutenea ulnaris. The RAPD technique was used to generate a DNA fingerprint of lines. Initially, a total of 35 primers having arbitrary sequences were used for RAPD analysis. The amplicons were resolved on 1.5% agarose gel and stained with ethidium bromide. To evaluate the bands, polymorphic and monomorphic bands were described. Genetic similarities and genetic distances were calculated in meat and layer pure lines. The RAPD analysis data from 23 primers were utilized in estimating genetic similarities, which ranged from 0.644 to 0.853 between the lines. The maximum genetic distance was observed between the L2 meat dam line and L4 layer sire line (0.440).

Estimation of Genetic Distance in Meat and Layer Pure Lines Using Randomly Amplified Polymorphic DNA

The purpose of this study was to evaluate genetic similarities and distances among pure lines of poultry using the RAPD technique. In this study four layer and three meat type pure lines were used. Venous blood samples from five males and five females of each line were collected from the venae cutenea ulnaris. The RAPD technique was used to generate a DNA fingerprint of lines. Initially, a total of 35 primers having arbitrary sequences were used for RAPD analysis. The amplicons were resolved on 1.5% agarose gel and stained with ethidium bromide. To evaluate the bands, polymorphic and monomorphic bands were described. Genetic similarities and genetic distances were calculated in meat and layer pure lines. The RAPD analysis data from 23 primers were utilized in estimating genetic similarities, which ranged from 0.644 to 0.853 between the lines. The maximum genetic distance was observed between the L2 meat dam line and L4 layer sire line (0.440).

Kaynak Göster

Bibtex @ { tbtkveterinary151867, journal = {Turkish Journal of Veterinary and Animal Sciences}, issn = {1300-0128}, eissn = {1303-6181}, address = {}, publisher = {TÜBİTAK}, year = {2002}, volume = {26}, pages = {1117 - 1120}, doi = {}, title = {Estimation of Genetic Distance in Meat and Layer Pure Lines Using Randomly Amplified Polymorphic DNA}, key = {cite}, author = {İvgin, Rahşan} }
APA İvgin, R . (2002). Estimation of Genetic Distance in Meat and Layer Pure Lines Using Randomly Amplified Polymorphic DNA . Turkish Journal of Veterinary and Animal Sciences , 26 (5) , 1117-1120 .
MLA İvgin, R . "Estimation of Genetic Distance in Meat and Layer Pure Lines Using Randomly Amplified Polymorphic DNA" . Turkish Journal of Veterinary and Animal Sciences 26 (2002 ): 1117-1120 <
Chicago İvgin, R . "Estimation of Genetic Distance in Meat and Layer Pure Lines Using Randomly Amplified Polymorphic DNA". Turkish Journal of Veterinary and Animal Sciences 26 (2002 ): 1117-1120
RIS TY - JOUR T1 - Estimation of Genetic Distance in Meat and Layer Pure Lines Using Randomly Amplified Polymorphic DNA AU - Rahşan İvgin Y1 - 2002 PY - 2002 N1 - DO - T2 - Turkish Journal of Veterinary and Animal Sciences JF - Journal JO - JOR SP - 1117 EP - 1120 VL - 26 IS - 5 SN - 1300-0128-1303-6181 M3 - UR - Y2 - 2021 ER -
EndNote %0 Turkish Journal of Veterinary and Animal Sciences Estimation of Genetic Distance in Meat and Layer Pure Lines Using Randomly Amplified Polymorphic DNA %A Rahşan İvgin %T Estimation of Genetic Distance in Meat and Layer Pure Lines Using Randomly Amplified Polymorphic DNA %D 2002 %J Turkish Journal of Veterinary and Animal Sciences %P 1300-0128-1303-6181 %V 26 %N 5 %R %U
ISNAD İvgin, Rahşan . "Estimation of Genetic Distance in Meat and Layer Pure Lines Using Randomly Amplified Polymorphic DNA". Turkish Journal of Veterinary and Animal Sciences 26 / 5 (Mayıs 2002): 1117-1120 .
AMA İvgin R . Estimation of Genetic Distance in Meat and Layer Pure Lines Using Randomly Amplified Polymorphic DNA. Turkish Journal of Veterinary and Animal Sciences. 2002; 26(5): 1117-1120.
Vancouver İvgin R . Estimation of Genetic Distance in Meat and Layer Pure Lines Using Randomly Amplified Polymorphic DNA. Turkish Journal of Veterinary and Animal Sciences. 2002; 26(5): 1117-1120.
IEEE R. İvgin , "Estimation of Genetic Distance in Meat and Layer Pure Lines Using Randomly Amplified Polymorphic DNA", Turkish Journal of Veterinary and Animal Sciences, c. 26, sayı. 5, ss. 1117-1120, May. 2002
  • ISSN: 1300-0128
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK

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