Comparison of Four Diagnostic Techniques for Detecting Bovine Virus Diarrhoea Virus (BVDV) in Buffy Coat Samples after Long-Term Storage

In this paper, four diagnostic techniques were compared in terms of their ability to detect BVD virus in buffy coats frozen for more than one year. The samples were selected according to their initial test results. Forty-five BVDV positive and 15 BVDV negative buffy coats were used. The techniques used were the virus isolation-immunoperoxidase test (IPX), immunoperoxidase monolayer assay (IPMA), indirect immunoperoxidase monolayer assay (IIPMA) and reverse transcription-polymerase chain reaction (RT-PCR). All BVDV negative samples from previous studies were also negative on the tests used in this study. Out of 45 BVDV-positive samples, 42 (93.3%) were found to be positive with RT-PCR, 41 (91.1%) with virus isolation-IPX, 40 (88.8%) with IIPMA and 37 (82.2%) with IPMA. The results obtained indicated that RT-PCR is more suitable for the detection of BVD virus in frozen buffy coats stored for more than one year when compared to other techniques, and IIPMA can be used for the routine diagnosis of BVDV infection as an alternative technique to virus isolation-IPX.

Comparison of Four Diagnostic Techniques for Detecting Bovine Virus Diarrhoea Virus (BVDV) in Buffy Coat Samples after Long-Term Storage

In this paper, four diagnostic techniques were compared in terms of their ability to detect BVD virus in buffy coats frozen for more than one year. The samples were selected according to their initial test results. Forty-five BVDV positive and 15 BVDV negative buffy coats were used. The techniques used were the virus isolation-immunoperoxidase test (IPX), immunoperoxidase monolayer assay (IPMA), indirect immunoperoxidase monolayer assay (IIPMA) and reverse transcription-polymerase chain reaction (RT-PCR). All BVDV negative samples from previous studies were also negative on the tests used in this study. Out of 45 BVDV-positive samples, 42 (93.3%) were found to be positive with RT-PCR, 41 (91.1%) with virus isolation-IPX, 40 (88.8%) with IIPMA and 37 (82.2%) with IPMA. The results obtained indicated that RT-PCR is more suitable for the detection of BVD virus in frozen buffy coats stored for more than one year when compared to other techniques, and IIPMA can be used for the routine diagnosis of BVDV infection as an alternative technique to virus isolation-IPX.

Kaynak Göster

Bibtex @ { tbtkveterinary151855, journal = {Turkish Journal of Veterinary and Animal Sciences}, issn = {1300-0128}, eissn = {1303-6181}, address = {}, publisher = {TÜBİTAK}, year = {2002}, volume = {26}, pages = {1043 - 1048}, doi = {}, title = {Comparison of Four Diagnostic Techniques for Detecting Bovine Virus Diarrhoea Virus (BVDV) in Buffy Coat Samples after Long-Term Storage}, key = {cite}, author = {Özkul, Aykut and Yeşilbağ, Kadir and Burgu, İbrahim} }
APA Özkul, A , Yeşilbağ, K , Burgu, İ . (2002). Comparison of Four Diagnostic Techniques for Detecting Bovine Virus Diarrhoea Virus (BVDV) in Buffy Coat Samples after Long-Term Storage . Turkish Journal of Veterinary and Animal Sciences , 26 (5) , 1043-1048 .
MLA Özkul, A , Yeşilbağ, K , Burgu, İ . "Comparison of Four Diagnostic Techniques for Detecting Bovine Virus Diarrhoea Virus (BVDV) in Buffy Coat Samples after Long-Term Storage" . Turkish Journal of Veterinary and Animal Sciences 26 (2002 ): 1043-1048 <
Chicago Özkul, A , Yeşilbağ, K , Burgu, İ . "Comparison of Four Diagnostic Techniques for Detecting Bovine Virus Diarrhoea Virus (BVDV) in Buffy Coat Samples after Long-Term Storage". Turkish Journal of Veterinary and Animal Sciences 26 (2002 ): 1043-1048
RIS TY - JOUR T1 - Comparison of Four Diagnostic Techniques for Detecting Bovine Virus Diarrhoea Virus (BVDV) in Buffy Coat Samples after Long-Term Storage AU - Aykut Özkul , Kadir Yeşilbağ , İbrahim Burgu Y1 - 2002 PY - 2002 N1 - DO - T2 - Turkish Journal of Veterinary and Animal Sciences JF - Journal JO - JOR SP - 1043 EP - 1048 VL - 26 IS - 5 SN - 1300-0128-1303-6181 M3 - UR - Y2 - 2021 ER -
EndNote %0 Turkish Journal of Veterinary and Animal Sciences Comparison of Four Diagnostic Techniques for Detecting Bovine Virus Diarrhoea Virus (BVDV) in Buffy Coat Samples after Long-Term Storage %A Aykut Özkul , Kadir Yeşilbağ , İbrahim Burgu %T Comparison of Four Diagnostic Techniques for Detecting Bovine Virus Diarrhoea Virus (BVDV) in Buffy Coat Samples after Long-Term Storage %D 2002 %J Turkish Journal of Veterinary and Animal Sciences %P 1300-0128-1303-6181 %V 26 %N 5 %R %U
ISNAD Özkul, Aykut , Yeşilbağ, Kadir , Burgu, İbrahim . "Comparison of Four Diagnostic Techniques for Detecting Bovine Virus Diarrhoea Virus (BVDV) in Buffy Coat Samples after Long-Term Storage". Turkish Journal of Veterinary and Animal Sciences 26 / 5 (Mayıs 2002): 1043-1048 .
AMA Özkul A , Yeşilbağ K , Burgu İ . Comparison of Four Diagnostic Techniques for Detecting Bovine Virus Diarrhoea Virus (BVDV) in Buffy Coat Samples after Long-Term Storage. Turkish Journal of Veterinary and Animal Sciences. 2002; 26(5): 1043-1048.
Vancouver Özkul A , Yeşilbağ K , Burgu İ . Comparison of Four Diagnostic Techniques for Detecting Bovine Virus Diarrhoea Virus (BVDV) in Buffy Coat Samples after Long-Term Storage. Turkish Journal of Veterinary and Animal Sciences. 2002; 26(5): 1043-1048.
IEEE A. Özkul , K. Yeşilbağ ve İ. Burgu , "Comparison of Four Diagnostic Techniques for Detecting Bovine Virus Diarrhoea Virus (BVDV) in Buffy Coat Samples after Long-Term Storage", Turkish Journal of Veterinary and Animal Sciences, c. 26, sayı. 5, ss. 1043-1048, May. 2002