Enver Barış BİNGÖL, Ömer ÇETIN, Hilal Çolak ÜZÜM, Hamparsun HAMPIKYAN

Effects of sodium lactate on the presence of Staphylococcus aureus and enterotoxins in çiğ köfte (raw meatball)

Çiğ köfte is a traditional raw meat product consumed almost everywhere in Turkey. It has a poor hygienic quality and can contain several pathogenic bacteria as no heating or cooking process is applied during any stage of manufacturing. The effects of sodium lactate on the presence of Staphylococcus aureus were investigated during the display-life of çiğ köfte and the formation of enterotoxins was also investigated. For this purpose, çiğ köfte samples were manufactured with different concentrations of sodium lactate (1%, 2%, 3%, and 4% NaL) and examined either in ambient (20 ± 2 °C) or refrigerator (4 ± 2 °C) storage. As a result, the shelf-life of çiğ köfte was improved with NaL addition; the growth of S. aureus was delayed dependent on NaL concentration used, and enterotoxin generations were retarded up to 12 h in cold storage.

Anahtar Kelimeler:

Key words: Çiğ köfte, sodium lactate, Staphylococcus aureus, enterotoxin, shelf-life

Effects of sodium lactate on the presence of Staphylococcus aureus and enterotoxins in çiğ köfte (raw meatball)

Keywords:

Key words: Çiğ köfte, sodium lactate, Staphylococcus aureus, enterotoxin, shelf-life,

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Öcal MH. Özellikleri ve Güzellikleriyle Çiğ Köftemiz. Şanlıurfa, Turkey: Özlem Press, 1997 (book in Turkish).
Durmaz H, Sagun E, Sancak H, Sagdic O. The fate of two Listeria monocytogenes serotypes in “cig kofte” at different storage temperatures. Meat Sci 2007; 76: 123–127.
Cetin O, Bingol EB, Akkaya H. The microbiological, serological and parasitological quality of cig kofte (raw meatball) and its lettuce marketed in Istanbul. Polish J Environ Stud 2008; 17: 701–706.
Sağun E, Alişarlı M, Durmaz H. The effect of different storage temperatures on the growth and enterotoxin producing characteristics of Staphylococcus aureus in çiğ köfte. Turk J Vet Anim Sci 2003; 27: 839–845.
Daglioglu F, Ilaslan N, Yilmaz I. The microbiological quality of cig kofte sold in Istanbul, Turkey. Fleischwirtschaft Int 2005; 1: 10–
Bingol EB, Cetin O, Muratoglu K. Effect of lemon Juice on the survival of Salmonella Enteritidis and Escherichia coli in cig kofte (raw meatball). Brit Food J 2011; 113: 1183–1194.
Gökmen M, Alişarlı M. Investigation of some pathogenic microorganisms in minced meat consumed in Van. J Fac Vet Med YYU 2003; 14: 27–34 (in Turkish with English abstract).
Hampikyan H, Bingol EB, Colak H, Aydin A. The evaluation of microbiological profile of some spices used in Turkish meat industry. J Food Agric Environ 2009; 7: 111–115.
Ardic M, Durmaz H. Determination of changes occurred in the microflora of cig kofte (raw meat balls) at different storage temperatures. Int J Food Sci Technol 2008; 43: 805–809.
Cetinkaya F, Mus TE, Cibik R, Levent B, Gulesen R. Assessment of microbiological quality of cig kofte (raw consumed spiced meatball): prevalence and antimicrobial susceptibility of Salmonella. Food Control 2012; 26: 15–18.
Mossel DAA, Van Netten P. Microbiological reference values for foods: a European perspective. J AOAC 1991; 74: 420–432.
Jablonski LM, Bohach G. Staphylococcus aureus. In: Doyle MP, Beuchat LR, Montville TJ editors. Food Microbiology: Fundamentals and Frontiers. Washington DC, USA: ASM Press; 2001. pp. 411–434.
US Food and Drug Administration. Foodborne Pathogenic Microorganisms and Natural Toxins. Rockville, MD, USA: Center for Food Safety and Applied Nutrition, US Food and Drug Administration, 1992.
Shelef LA. Antimicrobial effects of lactates: a review. J Food Protec 1994; 57: 445–450.
Bingöl EB, Bostan K. Usage of lactates in meat and meat products as a food additive. J Fac Vet Med İstanbul Univ 2012; 38: 79–88 (in Turkish with English abstract).
Harrigan WF, Laboratory Methods in Food Microbiology. London: Academic Press, 1998.
Davidson CM, Cronin F. Medium for the selective enumeration of lactic acid bacteria from foods. Appl Microbiol 1973; 26: 439–440.
AOAC. Official Methods of Analysis (Centennial Edition). Washington DC, USA: Association of Official Analytical Chemists, 1984.
International Organization for Standardization. ISO 6658. Sensory Analysis – Methodology – General Guidance. Paris, France: International Organization for Standardization, 1985.
Dufour M, Simmonds RS, Bremer PJ. Development of a method to quantify in vitro the synergistic activity of natural antimicrobials. Int J Food Microbiol 2003; 85: 249–258.
Koos JT. Preservation of food products with natural ingredients. Int Market Technol 1992; 3: 5–11.
Sallama KhI, Samejima K. Microbiological and chemical quality of ground beef treated with sodium lactate and sodium chloride during refrigerated storage. LWT 2004; 37: 865–871.
Walsh H, Martins S, O’Neill EE, Kerry JP, Kenny T, Ward P. The effect of sodium lactate, potassium lactate, carrageenan, whey protein concentrate, yeast extract and fungal proteinases on the cook yield and tenderness of bovine chuck muscles. Meat Sci 2010; 85: 230–234.
Küplülü Ö, Sarımehmetoğlu B, Oral N. The microbiological quality of çiğ köfte sold in Ankara. Turk J Vet Anim Sci 2003; 27: 325–329.
Sancak YC, İşleyici O. A study on the microbiological quality of çiğ köfte. J Fac Vet Med YYU 2006; 17: 81–86 (in Turkish with English abstract).
Erol İ, Mutluer B, Vatansever L. The detection of the ability to growth and enterotoxin production of Staphylococcus aureus in raw meat balls. Gida 1993; 18: 315–318.
Le Loir Y, Baron F, Gautier N. S. aureus and food poisoning. Genet Mol Res 2003; 2: 63–76.
Lavoie JP, Labbe RG, Chinachoti P. Growth of S. aureus as related to 17 O NMR water mobility and water activity. J Food Sci 1997; 62: 861–866.
McMahon CMM, Doherty AM, Sheridan JJ, Blair IS, McDowell DA, Hegarty T. Synergistic effect of heat and sodium lactate on the thermal resistance of Yersinia enterocolitica and Listeria monocytogenes in minced beef. Lett Appl Microbiol 1999; 28: 340–344.
Jung YJ, Min KJ, Yoon KS. Responses of acid-stressed Salmonella Typhimurium in broth and chicken patties to subsequent antimicrobial stress with 3-polylysine and combined potassium lactate and sodium diacetate. Food Microbiol 2009; 26: 467–474.