Alper ÇİFTCİ, Yağmur KOÇAK, Taylan ÖNYAY, Timur GÜLHAN, Arzu FINDIK, Merve Gizem SEZENER

Determination of methicillin resistance and some genotypic characteristics of staphylococci isolated from dogs and their owners

This study aimed to determine the methicillin resistance and some genotypic characteristics of staphylococci in dogs andtheir owners. A total of 132 swab samples from 33 healthy dogs and their owners were taken. Isolated staphylococci were identified byPCR. The antibiotic sensitivities of the isolates were determined by disc diffusion method. Determination of pvl, mecA, bla, and femgenes and SCCmec types was performed by PCR. Isolates were genotyped according to coa and spa gene polymorphisms by PCR. Fortyfive isolates were identified as Stapyloccoccus spp. Among them, 8 isolates were identified as S. aureus and 23 isolates were identified asS. pseudintermedius. According to the disc diffusion tests, methicillin resistance methicillin-resistant was determined in all S. aureus(MRSA). Among the methicillin-resistant strains, 8 were S. pseudintermedius (MRSP). However, methicillin resistance was determinedgenotypically in 10 strains and of these 5 were MRSA and 2 were MRSP. Two S. aureus strains and 7 S. pseudintermedius strains weremec A negative but bla positive. No strain carried the fem gene. Ten different coa types were detected among the strains. All MRSAstrains carried pvl genes. In conclusion, MRSP should be considered to pose a risk for humans living with dogs or in contact with them.

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1. Weese JS, van Duijkeren E. Methicillin resistant Staphylococcus aureus and Staphylococcus pseudintermedius in veterinary medicine. Vet Microbiol 2010; 140: 418-429.
2. Pereira IA, da Motta CC, Rojas ACCM, Coelho IS, Coelho SMO, de Souza MMS. Challanges in predicting Staphylococcus spp. β-lactamic resistance in pet animals. Afr J Microbiol Res 2014; 8: 3662-3669.
3. deLencastre H, Tomasz A. Reassessment of the number of auxiliary genes essential for expression of high level methicillin resistance in Staphylococcus aureus. Antimicrob Agent Chemother 1994; 38: 2590-2598.
4. Fitzgerald DB. The Staphylococcus intermedius group of bacterial pathogens: species reclassification, pathogenesis and emergence of methicillin resistance. Vet Dermatol 2009; 20: 490-495.
5. Markey B, Leonard F, Archambault M, Cullianane A, Maguire D. Staphylococcus. In: Clinical Veterinary Microbiology, 2nd ed. Amsterdam, Netherlands: Mosby Elsevier; 2013. pp. 105- 119.
6. Çiftci A, Fındık A, Onuk EE, Savasan S. Detection of methicillin resistance and slime factor production of Staphylococcus aureus in bovine mastitis. Braz J Microbiol 2009; 40: 254-261.
7. Bannoehr J, Franco A, Luresca M, Battisti A, Fitzgerald JR. Molecular diagnostic identification of Staphylococcus pseudintermedius. J Clin Microbiol 2009; 47: 469-471.
8. Clinical and Laboratory Standards Institute (CLSI). Performance standards for antimicrobial susceptibility testing. Twenty-first informational supplement approved standard M100-S21, v. 31, Wayne, PA, USA; 2011. pp. 76-79.
9. Boye K, Bartels MD, Andersen IS, Møller JA, Westh H. A new multiplex PCR for easy screening of methicillin-resistant Staphylococcus aureus SCCmec types I–V. Clin Microbiol Infect 2007; 13: 725-727.
10. Fındık A, Akan N, Onuk EE, Çakıroğlu D, Çiftci A. Methicillin resistance profile and molecular typing of Staphylococcus aureus strains isolated from the noses of healthy dogs. Kafkas Univ Vet Fak 2009; 15: 925-930.
11. Montesinos I, Salido E, Delgado T, Cuervo M, Sierra A. Epidemiologic genotyping of methicillin-resistant Staphylococcus aureus by pulsed-field gel electrophoresis at a University Hospital and comparison with antibiotyping and protein A and coagulase gene polymorphisms. J Clin Microbiol 2002; 40: 2119-2125.
12. Azimian A, Havaei SA, Fazeli H, Naderi M, Ghazvini K, Samiee SM, Soleimani M, Peerayeh SN. Genetic characterization of a vancomycin-resistant Staphylococcus aureus isolate from the respiratory tract of a patient in a university hospital in northeastern Iran. J Clin Microbiol 2012; 50: 3581-3585.
13. Loeffler A, Lloyd DH. Companion animals: a reservoir for methicillin-resistant Staphylococcus aureus in the community? Epidemiol Infect 2010; 138: 595-605.
14. Guardabassi L, Loeber M, Jacobson A. Transmission of multiple antimicrobial-resistant Staphylococcus intermedius between dogs affected by deep pyoderma and their owners. Vet Microbiol 2004; 98: 23-27.
15. Baptiste KE, Williams K, Willams NJ, Wattret A, Clegg PD, Dawson S, Corkill JE, O’Neill T, Hart CA. Methicillin-resistant staphylococci in companion animals. Emerg Infect Dis 2005; 11: 1942-1944.
16. Hanselman BA, Kruth S, Weese JS. Methicillin-resistant staphylococcal colonization in dogs entering a veterinary teaching hospital. Vet Microbiol 2008; 26: 277-281.
17. Griffeth GC, Morris DO, Abraham JL, Shofer FS, Rankin SC. Screening for skin carriage of methicillin-resistant coagulasepositive staphylococci and Staphylococcus schleiferi in dogs with healthy and inflamed skin. Vet Dermatol 2008; 19: 142-149.
18. Sasaki T, Kikuchi K, Tanaka Y, Takahashi N, Kamata S, Hiramatsu K. Methicillin-resistant Staphylococcus pseudintermedius in a veterinary teaching hospital. J Clin Microbiol 2007; 45: 1118-1125.
19. Morris DO, Boston RC, O’Shea K, Rankin SC. The prevalence of carriage of methicillin-resistant staphylococci by veterinary dermatology practice staff and their respective pets. Vet Dermatol 2010; 21: 400-407.
20. Chikkala R, George NO, Ratnakar KS, Iyer RN, Sritharan V. Heterogeneity in femA in the Indian isolates of Staphylococcus aureus limits its usefulness as a species specific marker. Adv Infect Dis 2012; 2: 82-88.
21. Akineden O, Hassan AA, Schneider E, Usleber E. A coagulasenegative variant of Staphylococcus aureus from bovine mastitis milk. J Dairy Res 2011; 78: 38-42.
22. Van Duijkeren E, Wolfhagen MJ, Heck MEOC, Wannet WJB. Transmission of a Panton–Valentine Leucocidin positive methicillin-resistant Staphylococcus aureus strain between humans and a dog. J Clin Microbiol 2005; 43: 6209-6211.
23. Rankin S, Roberts S, O’Shea K, Maloney D, Lorenzo M, Benson CE. Panton–Valentine Leukocidin (PVL) toxin positive MRSA strains isolated from companion animals. Vet Microbiol 2005; 108: 145-148.
24. Gandolfi-Decristophoris P, Regula G, Petrini O, Zinsstag J, Schelling E. Prevalence and risk factors for carriage of multidrug resistant staphylococci in healthy cats and dogs. J Vet Sci 2013; 14: 449-456.
25. Loeffler A, Linek M, Moodley A, Guardabassi L, Sung JM, Winkler M, Weiss R, Lloyd DH. First report of multiresistant, mecA-positive Staphylococcus intermedius in Europe: 12 cases from a veterinary dermatology referral clinic in Germany. Vet Dermatol 2007; 18: 412-421.
26. van Duijkeren E, Catry B, Greko C, Moreno MA, Pomba MC, Pyörälä S, Ružauskas M, Sanders P, Threlfall EJ, TorrenEdo J et al. Review on methicillin-resistant Staphylococcus pseudintermedius. J Antimicrob Chemother 2011; 66: 2705- 2714.