The first-time isolation of Brucella canis from two aborted bitches in a kennel in Turkey

Brucella canis is defined as the specific agent that is responsible for canine brucellosis. In this study, B. canis was isolated andidentified from the clinical materials belonging to 2 dogs kept in a breeding kennel in Turkey and brought to the researchers’ clinic withabortion complaints. With this study, for the first time ever, B. canis isolation and identification was made from dogs that aborted in akennel. This study emphasizes the necessity to consider and evaluate dog abortions that occur for unspecified reasons within the scopeof brucellosis.

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1. Viana MVC, Wattam AR, Govil Batra D, Boisvert S, Brettin TS, Frace M, Xia F, Azevedo V, Tiller R, Hoffmaster AR. Genome sequences of three Brucella canis strains isolated from humans and a dog. Genome Announc 2017; 5: 1-2.
2. Holst BS, Löfqvist K, Ernholm L, Eld K, Cedersmyg M, Hallgren G. The first case of Brucella canis in Sweden: background, case report and recommendations from a northern European perspective. Acta Vet Scand 2012; 54: 1-9.
3. Diker S, İstanbulluoğlu E, Ayhan H, Soysal GA. Sero-survey of Brucella canis infections in man at Bursa district. Mikrobiyol Bült 1984; 18: 203-207 (in Turkish with abstract in English).
4. Karaşahin Ö, İba-Yılmaz S, Aslan MH, Çelik N. Infective endocarditis due to Brucella canis: a case report. Klimik Derg 2016; 29: 138-141 (in Turkish with abstract in English).
5. Aras Z, Uçan US. Detection of Brucella canis from inguinal lymph nodes of naturally infected dogs by PCR. Theriogenology 2010; 74: 658-662.
6. Aras Z, Taşpınar M, Aydın İ. A novel polymerase chain reaction to detect Brucella canis in dogs. Kafkas Univ Vet Fak 2015; 21: 169-172.
7. Yumuşak N, Polat PF, Erdenliğ Gürbilek S, Şen ÇC, Yılmaz R. A comparison of the performance of exfoliative cytopathology, polymerase chain reaction (PCR), culture and ELISA in the detection of Brucella canis. Harran Üniversitesi Veteriner Fakültesi Dergisi 2017; 6: 51-56.
8. Alton GG, Jones LM, Angus RD, Verger JM. Techniques for the Brucellosis Laboratory. Paris, France: Institut National de la Recherche Agronomique; 1988.
9. World Organisation for Animal Health. Terrestrial Manual. Chapter 2.1.4. Brucellosis. Paris, France: OIE; 2016.
10. Queipo-Ortuno MI, Morata P, Ocon P, Manchado P, Colmenero JD. Rapid diagnosis of human brucellosis by peripheral blood PCR assay. J Clin Microbiol 1997; 35: 2927-2930.
11. Mayer-Scholl A, Draeger A, Golner C, Scholz HC, Nockler K. Advancement of a multiplex PCR for the differentiation of all currently described Brucella species. J Microbiol Methods 2010; 80: 112-114.
12. Keid LB, Chiebao DP, Batinga MCA, Faita T, Diniz JA, Oliveria TMFS, Ferreira HL, Soares RM. Brucella canis infection in dogs from commercial breeding kennels in Brazil. Transbound Emerg Dis 2017; 64: 691-697.
13. Sanchez-Jimenez MM, Ortiz-Roman LF, Castrillon-Salazar LL, Giraldo-Echeverri CA, Olivera-Angel M. Application of a polymerase chain reaction test for the detection of Brucella canis from clinical samples of canines and humans. Rev Colomb Cienc Pec 2014; 27: 3-11.
14. Sayan M, Erdenlig S, Stack J, Kilic S, Guducuoglu H, Aksoy HY, Baklan EA, Etiler N. A serological diagnostic survey for Brucella canis infection in Turkish patients with brucellosislike symptoms. Jpn J Infect Dis 2011; 64: 516-519.
15. Lopez-Goni I, Garcia-Yoldi D, Marin CM, De Miguel MJ, Munoz PM, Blasco JM, Jacques I, Grayon M, Cloeckaert A, Ferreira AC et al. Evaluation of a multiplex PCR assay (Bruceladder) for molecular typing of all Brucella species and of the vaccine strains. J Clin Microbiol 2008; 46: 3484-3487.