Siddharth GAUTAM, Amol GURAV, Chandrakanta JANA, Anil Kumar SHARMA

Morphological diagnosis of Johne’s disease: a case report in cattle

The present case report deals with the morphological (gross and histopathological) and histochemical characterization of Johne?s disease in a cow. The carcass was presented in a hidebound condition with sunken eyes and with a history of persistent and nonresponsive diarrhea. Necropsy revealed generalized gelatinization of the visceral and peripheral fat. Thorough gross examination unveiled major pathological changes in the digestive tract. The gallbladder was distended, with severely thickened walls and blood mixed with bile. The small intestine was severely congested and the mesenteric lymph nodes were enlarged. The opening of the small intestine showed marked thickening of the intestinal walls, with a corrugated appearance. Diffuse granulomatous lesions were observed in the mesenteric lymph nodes. Histopathological examination revealed severe infiltration of intestinal layers and mesenteric lymph nodes by macrophages, lymphocytes, and epithelioid cells with multiple Langhans giant cells. Ziehl=Neelsen staining demonstrated multiple acid-fast bacilli in the cytoplasm of macrophages from impression smears.

Keywords:

Paratuberculosis Crohn’s disease, giant cells, cattle, epithelioid cell,

PDF

___

Al Hajri SM, Alluwaimi AM. ELISA and PCR for evaluation of subclinical paratuberculosis in the Saudi dairy herds . Vet Microbiol 2007; 121: 384-385.
Stabel JR. Johne’s disease: a hidden threat . J. Dairy Sci 1997; 81: 283-288.
Stabel JR, Wells SJ, Wagner BA. Relationships between fecal culture, ELISA, and bulk tank milk test results for Johne’s disease in US dairy herds . J Dairy Sci 2002; 85: 525-531.
Whittington RJ, Windsor PA. In utero infection of cattle with Mycobacterium avium subsp. paratuberculosis : a critical review and meta-analysis . Vet J 2009; 179: 60-69.
Naser SA, Hulten K, Shafran I, Graham DY, El-Zaatari FA. Specific seroreactivity of Crohn’s disease patients against p35 and p36 antigens of M. avium subsp. paratuberculosis. Vet Microbiol 2000; 77: 497-504.
Whitlock RH, Buergelt C. Preclinical and clinical manifestations of paratuberculosis (including pathology) . Vet Clin North Am Food Anim Pract 1996; 12: 345-356.
Mahmoud OM, Haroun EM, Elfaki MG, Abbas B. Pigmented paratuberculosis granulomata in the liver of sheep . Small Rumin Res 2002; 43: 211- 217.
Perez V, Garcia Marin JF, Badiola JJ. Description and classification of different types of lesion associated with natural paratuberculosis infection in sheep . J Comp Pathol 1996; 114: 107-122.
Smith SL, Wilson PR, Collett MG, Heuer C, West DM, Stevenson M, Chambers JP. Liver biopsy histopathology for diagnosis of Johne’s disease in sheep . Vet Pathol 2014; 51: 915-918.
Clarke CJ, Little D. The pathology of ovine paratuberculosis: gross and histological changes in the intestine and other tissues . J Comp Pathol 1996; 114: 419-437.
Singh AV, Singh SV, Singh PK, Sohal JS, Singh MK. High prevalence of Mycobacterium avium subspecies paratuberculosis (‘Indian bison type’) in animal attendants suffering from gastrointestinal complaints who work with goat herds endemic for Johne’s disease in India . Int J Infect D is 2011; 15: e677-683.