Şükrü KIRKAN, Osman KAYA, Serten TEKBIYIK, Uğur PARIN

Detection of Coxiella burnetii in Cattle by PCR

This study is aimed to detect Coxiella burnetii in cattle by Polymerase Chain Reaction (PCR). A total of 138 cattle blood samples were collected from 8 farms and then examined in laboratory conditions. The examination of Coxiella burnetii was carried out by PCR with specific primers. In this study, a total of 6 (4.3%) cattle serum samples were found PCR positive for Coxiella burnetii. This result proves that cattle are an important reservoir of Coxiella burnetii infection.

Anahtar Kelimeler:

Coxiella burnetii, cattle, PCR

Detection of Coxiella burnetii in Cattle by PCR

Keywords:

Coxiella burnetii, cattle, PCR,

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Field, P.R., Mitchell, J.L., Santiago, A., Dickeson, D.J., Chan, S.W., Ho, D.W.T., Murphy, A.M., Cuzzubbo, A.J., Devine, P.L.: Comparison of a commercial enzyme-linked immunosorbent assay with immunofluorescence and complement fixation tests for detection of Coxiella burnetii (Q fever) immunoglobulin M. J. Clin. Microbiol., 2000; 38: 1645-1647.
Zhang, G.Q., Hotta, A., Mizutani, M., Ho, T., Yamaguchi, T., Fukushi, H., Hirai, K.: Direct identification of Coxiella burnetii plasmids in human sera by nested PCR. J. Clin. Microbiol., 1998; 36: 2210-2213.
Nguyen, S.V., Hirai, K.: Differentiation of Coxiella burnetii isolates by sequence determination and PCR-restriction fragment length polymorphism analysis of isocitrate dehydrogenase gene. FEMS Microbiol. Lett., 1999; 180: 249-254.
Heinzen, R.A., Frazier, M.E., Mallavia, L.P.: Nucleotide sequence of Coxiella burnetii superoxide dismutase. Nucleic Acids Res., 1990; 18: 6437.
Hoover, T.A., Vodkin, M.H., Williams, J.C.: A Coxiella burnetii repeated DNA element resembling a bacterial insertion sequence. J. Bacteriol., 1992; 174: 5540-5548.
Berri, M., Laroucau, K., Rodolakis, A.: The detection of Coxiella burnetii from ovine genital swabs, milk and fecal samples by the use of a single touchdown polymerase chain reaction. Vet. Microbiol., 2000; 72: 285-293.
Raoult, D.: Host factors in the severity of Q fever. Ann. N. Y. Acad. Sci., 1990; 590: 33-38.
Tissot-Dupont, H., Raoult, D., Brouqui, P., Janbon, F., Peyramond, D., Weiller, P.J., Chicheportiche, C., Nezri, M., Poirier, R.: Epidemiologic features and clinical presentation of acute Q fever in hospitalized patients: 323 French cases. Am. J. Med., 1992; 93: 427-434.
Aguirre Errasti, C., Montejo Baranda, M., Hernandez Almaraz, J.L., delaHoz Torres, C., Martinez Gutierrez, E., Villate Navarro, J.L., Sobradillo Peña, V.: An outbreak of Q fever in the Basque country. Can. Med. Assoc. J., 1984; 131: 48-49.
Guigno, D., Coupland, B., Smith, E.G., Farrell, I.D., Desselberger, U., Caul, E.O.: Primary humoral antibody response to Coxiella burnetii, the causative agent of Q fever. J. Clin. Microbiol., 1992; 30: 1958-1967.
To, H., Htwe, K.K., Kako, N., Kim, H.J., Yamaguchi, T., Fukushi, H., Hirai, K.: Prevalence of Coxiella burnetii infection in dairy cattle with reproductive disorders. J. Vet. Med. Sci., 1998; 60: 859-861.
McQuiston J.H., Childs, J.E.: Q fever in humans and animals in the United States. Vector Borne Zoonotic Dis., 2002; 2: 179-191.
Kim, S.G., Kim, E.H., Lafferty, C.J., Dubovi, E.:Coxiella burnetii in bulk tank milk samples, United States, Emerg. Infect. Dis., 2005; 11: 619-621.
Berri, M., Souriau, A., Crosby, M., Rodolakis, A.: Shedding of Coxiella burnetii in ewes in two pregnancies following an episode of Coxiella abortion in a sheep flock. Vet. Microbiol., 2002; 85: 55-60.
Parisi, A., Fraccalvieri, R., Cafiero, M., Miccolupo, A., Padalino, I., Montagna, C., Capuano, F., Sottili, R.: Diagnosis of Coxiella burnetii-related abortion in Italian domestic ruminants using single-tube nested PCR. Vet. Microbiol., 2006; 118: 101-106.
Sting, R., Kopp, J., Mandl, J., Seeh, C., Seemann, G., Kimmig, P., Schmitt, K., Mentrup, T.: Studies of Coxiella burnetii infections in dairy herds with special regard to infections in men. Berl. Munch. Tierarztl. Wochenschr., 2002; 115: 360-365 (article in German with an abstract in English).
Hamzi´c, S., Beslagi´c, E., Puvaci´c, S., Aljicevi´c, M.: Coxiella burnetii infections as the public-health problem. Med. Arh., 2005; 59: 3- 6. (article in Bosnian with an abstract in English).
Abe, T., Yamaki, K., Hayakawa, T., Fukuda, H., Ito, Y., Kume, H., Komiya, T., Ishihara, K., Hirai, K.: A seroepidemiological study of the risks of Q fever infection in Japanese veterinarians. Eur. J. Epidemiol., 2001; 17: 1029-1032.
Seyitoğlu, S., Özkurt, Z., Dinler, U., Okumuş, B.: The seroprevalence of Coxiellosis in farmers and cattle in Erzurum district in Turkey. Turk. J. Vet. Anim Sci., 2006; 30: 71-75.
Özgür, N.Y., Hasöksüz, M., Yılmaz, H., İkiz, S., Ilgaz, A.: Detection of antibodies to Coxiella burnetii in cattle with infertility problems and human sera by ELISA and its seroprevalence. Pendik Vet. Mikrobiyol. Derg., 1997; 28: 207-218.
Cetinkaya, B., Kalender, H., Ertas, H.B., Muz, A., Arslan, N., Ongor, H., Gurcay, M.: Seroprevalence of Coxiellosis in cattle, sheep and people in the east of Turkey. Vet. Rec., 2000; 146: 131-136.
Brouqui, P., Dumler, J.S., Raoult, D.: Immunohistologic demonstration of Coxiella burnetii in the valves of patients with Q fever endocarditis. Am. J. Med., 1994; 97: 451-458.