Comparative detection of bovine herpesvirus-1 using antigen ELISA, immunohistochemistry and immunofluorescence methods in cattle with pneumonia
Comparative detection of bovine herpesvirus-1 using antigen ELISA, immunohistochemistry and immunofluorescence methods in cattle with pneumonia
This study was conducted to detect the prevalence and presence of bovine herpesvirus-1 (BoHV-1) using immunohistochemistry(IHC), immunofluorescence (IF) and antigen enzyme-linked immunosorbent assay (ELISA) in the lung samples of cattle (n = 1023). Inaddition, three methods were compared using receiver operating characteristic (ROC) and chi-square test (χ2 test), and their usabilitywere evaluated in laboratory conditions. Macroscopically, pneumonia was seen in 120 of the lung tissue samples (11.73%). Basedon the microscopic examinations, the pneumonia types were classified as catarrhal-suppurative bronchopneumonia (6.7%), fibrinousbronchopneumonia (5%), interstitial pneumonia (84.1%), and granulomatous pneumonia (4.2%). The IHC, IF and antigen ELISApositivity were 48.33%, 50.83%, and 29.9%, respectively. Considering IF as a gold standard, IHC had more acceptable sensitivity andspecificity than antigen ELISA. In conclusion, it has been observed that when IF method is not available, IHC is more reliable thanantigen ELISA in the diagnosis of BoHV-1.
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- 1. Hartel H, Nikunen S, Neuvonen E, Tanskanen R, Kivela SL,
Aho R, Soveri T, Saloniemi H. Viral and bacterial pathogens in
bovine respiratory disease in Finland. Acta Vet Scand 2004; 45:
193-200.
- 2. EL-Bayoumy MK AK, Elgayed SS, Allam AM, Taha SS.
Antiviral competence of broad-spectrum antiinfective drug
artesunate against Bovine Herpesvirus-1. International Journal
of Basic and Applied Virology 2013; 2: 6-13.
- 3. López A. Respiratory system. In: McGavin MD, Zachary JF,
editors. Pathologic Basis of Veterinary Disease. 4th ed. St.
Louis, Missouri, USA: Elsevier Health Sciences; 2006. pp. 463-
473.
- 4. Griffin D, Chengappa M, Kuszak J, McVey DS. Bacterial
pathogens of the bovine respiratory disease complex. Vet Clin
North Am Food Anim Pract. 2010; 26: 381-394.
- 5. O’Neill R, Mooney J, Connaghan E, Furphy C, Graham D.
Patterns of detection of respiratory viruses in nasal swabs from
calves in Ireland: a retrospective study. Vet Rec 2014; 175: 351-
351.
- 6. Horwood PF, Mahony TJ. Multiplex real-time RT-PCR
detection of three viruses associated with the bovine respiratory
disease complex. J Virol Methods. 2011; 171: 360-363.
7. Mosier D. Review of BRD pathogenesis: the old and the new.
Anim Health Res Rev 2014; 15: 166-168.
- 8. Jain L. Detection of bovine herpes virus-1 (BHV-1) infection
in breeding bulls by serological and molecular methods and
its characterization by sequencing of PCR products. Msc,
College of Veterinary Science and Animal Husbandry Anand
Agricultural University, Anand, India, 2006.
- 9. Surendra K, Rana S, Subramanian B, Reddy R, Sharma G,
Srinivasan V. Phylogenetic analysis of bovine herpesvirus
isolate of India. Advances in Animal and Veterinary Sciences
2015; 3: 451-460.
- 10. Engels M, Ackermann M. Pathogenesis of ruminant
herpesvirus infections. Vet Microbiol 1996; 53: 3-15.
- 11. Nandi S, Kumar M, Manohar M, Chauhan R. Bovine herpes
virus infections in cattle. Anim Health Res Rev 2009; 10: 85-98.
- 12. Muylkens B, Thiry J, Kirten P, Schynts F, Thiry E. Bovine
herpesvirus 1 infection and infectious bovine rhinotracheitis.
Vet Res 2007; 38: 181-209.
- 13. Biswas S, Bandyopadhyay S, Dimri U, H. Patra P. Bovine
herpesvirus-1 (BHV-1) are emerging concern in livestock: a
revisit to its biology, epidemiology, diagnosis, and prophylaxis.
Vet Q 2013; 33: 68-81.
- 14. Moore S, Gunn M, Walls D. A rapid and sensitive PCR-based
diagnostic assay to detect bovine herpesvirus 1 in routine
diagnostic submissions. Vet Microbiol 2000; 75: 145-153.
- 15. Turin L, Russo S, Poli G. BoHV-1: new molecular approaches
to control a common and widespread infection. Mol Med 1999;
5: 261-284,.
- 16. Rola J, Larska M, Polak MP. Detection of bovine herpesvirus 1
from an outbreak of infectious bovine rhinotracheitis. Bull Vet
Inst Pulawy 2005; 49: 267.
- 17. De-Giuli L, Magnino S, Vigo PG, Labalestra I, Fabbi M.
Development of a polymerase chain reaction and restriction
typing assay for the diagnosis of bovine herpesvirus 1, bovine
herpesvirus 2, and bovine herpesvirus 4 infections. J Vet Diagn
Invest 2002; 14: 353-356.
- 18. Yates W. A review of infectious bovine rhinotracheitis, shipping
fever pneumonia and viral-bacterial synergism in respiratory
disease of cattle. Can J Comp Med 1982; 46: 225-263.
- 19. Mehana E-SE. An outbreak of Bovine Respiratory Disease in
calves: Clinical and Histopathological findings. IOSR-JAVS.
2014; 6.
- 20. Kirkland PD, Gu X. Infectious Bovine Rhinotracheitis.
Canberra, Australia: Australian and New Zealand Standard
Diagnostic Procedures Sub-committee on Animal Health
Laboratory Standards, Animal Health Australia; 2008.
- 21. Wągrowska-Danilewicz M, Danilewicz M. Immunofluorescence
on paraffin-embedded sections in evaluation of immune
complex deposits in renal biopsy specimens. Pol J Pathol 2009;
60: 3-9.
- 22. Kajimura J, Ito R, Manley NR, Hale LP. Optimization of singleand
dual-color immunofluorescence protocols for formalinfixed,
paraffin-embedded archival tissues. J Histochem
Cytochem 2016; 64: 112-124.
- 23. Alexander B, MacVean D, Salman M. Risk factors for lower
respiratory tract disease in a cohort of feedlot cattle. J Am Vet
Med Assoc 1989; 195: 207-211.
- 24. Caldow G, Edwards S, Nixon P, Peters A. Associations between
viral infection and respiratory disease in young beef bulls. Vet
Rec 1988; 122: 529-531.
- 25. Özer H. Besi sığırlarında atipik interstitial pneumonie’lerin
yayılışı. Fırat Üniversitesi Sağlık Bilimleri Dergisi 1987; 1: 27-
34.
- 26. Ortatatlı M, Çiftçi MK. Konya bölgesi mezbahalarında
kesilen besi danalarında pnömonilerin insidensi ve patoloji.
Veterinarium 2005; 16: 24-35.
- 27. Narita M, Kimura K, Tanimura N, Arai S, Tsuboi T, Katsuda
K. Immunohistochemical characterization of calf pneumonia
produced by the combined endobronchial administration of
bovine herpesvirus 1 and Pasteurella haemolytica. J Comp
Pathol 2000; 123: 126-134.
- 28. Loneragan GH, Gould DH, Mason GL, Garry FB, Yost GS,
Miles DG, Mills LJ. Involvement of microbial respiratory
pathogens in acute interstitial pneumonia in feedlot cattle. Am
J Vet Res 2001; 62: 1519-1524.
- 29. Sayers RG. Associations between exposure to bovine
herpesvirus 1 (BoHV-1) and milk production, reproductive
performance, and mortality in Irish dairy herds. J Dairy Sci
2017; 100: 1340-1352.
- 30. Murray GM, More SJ, Sammin D, Casey MJ, McElroy MC,
O’Neill RG, Byrne WJ, Earley B, Clegg TA, Ball H et al. Cassidy
Pathogens, patterns of pneumonia, and epidemiologic risk
factors associated with respiratory disease in recently weaned
cattle in Ireland. J Vet Diagn Invest 2017; 29: 20-34.
- 31. Majumder S, Ramakrishnan M, Nandi S. Infectious Bovine
Rhinotracheitis: An Indian Perspective. IJCMAS 2015; 4: 844-
858.
- 32. Boelaert F, Biront P, Soumare B, Dispas M, Vanopdenbosch
E, Vermeersch J, Raskin A, Dufey J, Berkvens D, Kerkhofs
P. Prevalence of bovine herpesvirus-1 in the Belgian cattle
population. Prev Vet Med 2000; 45: 285-295.
- 33. Yan B, Chao Y, Chen Z, Tian K, Wang C, Lin X, Chen H, Guo
A. Serological survey of bovine herpesvirus type 1 infection in
China. Vet Microbiol 2008; 127: 136-141.
- 34. Rypuła K, Płoneczka-Janeczko K, Kita J, Kumala A, Żmudziński
J. Seroprevalence of BHV-1 (bovine herpesvirus type 1) among
non-vaccinated dairy cattle herds with respiratory disorders.
Pol J Vet Sci 2012; 15: 561-563.
- 35. Nandi S, Kumar M, Yadav V, Chander V. Serological evidences
of bovine herpesvirus-1 infection in bovines of organized
farms in India. Transbound Emerg Dis 2011; 58: 105-109.
- 36. Cavinari S. Epidemiological data on IBR in Italy and experience
of control in the field. Proceedings: BHV-1 eradication
Symposium Berlin, Germany. 2006; pp. 8-9.
- 37. Alkan F, Burgu I, Bilge-Dagalp S, Yıldırım Y, Gencay A, Güngör
B, Ataseven V, Akça Y. The seroprevalence of BHV-1 infection
on selected dairy cattle herds in Turkey. Rev Med Vet-Toulouse
2005; 156: 166-169.
- 38. Tan M, Yıldırım Y, Erol N, Güngör A. The seroprevalance of
Bovine Herpesvirus type 1 (BHV-1) and Bovine Leukemia
Virus (BLV) in Selected Dairy Herds in Aydın Province,
Turkey. Turk J Vet Anim Sci 2006; 30: 323-357.
- 39. Okur-Gumusova S, Yazici Z, Albayrak H, Cakiroglu D.
Seroprevalence of bovine viral respiratory diseases. Acta Vet-
Beograd 2007; 57: 11-16.
- 40. Yıldırım Y, Burgu İ. The seroprevalence of bluetongue (BT),
IBR, PI-3, EBL and BVD infections in cattle in northeastern
Anatolia. Ankara Univ Vet Fak Derg 2005; 52: 113-117.
- 41. Collins JK, Ayers VK, Carman J. Evaluation of an antigencapture
ELISA for the detection of bovine herpesvirus type 1
shedding from feedlot cattle. Vet Microbiol. 1988; 16: 101-107.
- 42. Elhassan AM, Fadol MA, Salih MAM, El Hussein ARM.
Bovine herpes virus-1 (BoHV-1) detection in dairy cattle with
reproductive problems in Sudan. J Adv Vet Anim Res 2015; 2:
185-189.
- 43. Saeed IK, Ali YH, AbdulRahman MB, Mohammed ZA, Osman
HM, Taha KM, Musa MZ, Khalafalla AI. Mixed infection of
peste des petits ruminants virus (PPRV) and other respiratory
viruses in dromedary camels in Sudan, an abattoir study. Trop
Anim Health Prod 2015; 47: 995-998.
- 44. Avci O, Yavru S, Ekik M. Detection of respiratory viral antigens
in cattle lung tissues by direct ELISA. Animal and Veterinary
Sciences 2014; 2: 146-149.
- 45. Crook T, Benavides J, Russell G, Gilray J, Maley M, Willoughby
K. Bovine herpesvirus 1 abortion: current prevalence in the
United Kingdom and evidence of hematogenous spread within
the fetus in natural cases. J Vet Diagn Invest 2012; 24: 662-670.
- 46. Szeredi L, Jánosi S, Pálfi V. Microbiological and pathological
examination of fatal calf pneumonia cases induced by bacterial
and viral respiratory pathogens. Acta Vet Hung 2010; 58: 341-
356.
- 47. Ceribasi A, Ozkaraca M, Ceribasi S, Ozer H. Histopathologic,
immunoperoxidase and immunofluorescent examinations
on natural cattle pneumonia originated from Parainfluenza
type 3, Respiratory Syncytial virus, Adenovirus type 3 and
Herpesvirus type 1. Rev Med Vet.2014; 165: 201-212.
- 48. Intisar K, Ali Y, Khalafalla A, Mahasin ER, Amin A. Natural
exposure of Dromedary camels in Sudan to infectious bovine
rhinotracheitis virus (bovine herpes virus-1). Acta Trop 2009;
111: 243-246.
- 49. Cornish TE, van Olphen AL, Cavender JL, Edwards JM, Jaeger
PT, Vieyra LL, Woodard LF, Miller DR, O’Toole D. Comparison
of ear notch immunohistochemistry, ear notch antigen-capture
ELISA, and buffy coat virus isolation for detection of calves
persistently infected with bovine viral diarrhea virus. J Vet
Diagn Invest 2005; 17: 110-117.